QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (39) Citing Articles via Google Scholar Google Scholar Articles by Neve, R. L. Articles by Lynn, S. D. Search for Related Content PubMed PubMed Citation Articles by Neve, R. L. Articles by Lynn, S. D.

 Previous Article  |  Next Article 

The Journal of Neuroscience, October 1, 1998, 18(19):7757-7767

The Neuronal Growth-Associated Protein GAP-43 Interacts with Rabaptin-5 and Participates in Endocytosis

Rachael L. Neve^{1, 2}, Robert Coopersmith^{1, 2}, Donna L. McPhie^{1, 2}, Christopher Santeufemio², Kara G. Pratt^{1, 2}, Curran J. Murphy^{1, 2}, and Stephanie D. Lynn^{1, 2}

¹ Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, and ² McLean Hospital, Belmont, Massachusetts 02178

Structural plasticity of nerve cells is a requirement for activity-dependent changes in the brain. The growth-associated protein GAP-43 is thought to be one determinant of such plasticity, although the molecular mechanism by which it mediates dynamic structural alterations at the synapse is not known. GAP-43 is bound by calmodulin when Ca²⁺ levels are low, and releases the calmodulin when Ca²⁺ levels rise, suggesting that calmodulin may act as a negative regulator of GAP-43 during periods of low activity in the neurons. To identify the function of GAP-43 during activity-dependent increases in Ca²⁺ levels, when it is not bound to calmodulin, we sought proteins with which GAP-43 interacts in the presence of Ca²⁺. We show here that rabaptin-5, an effector of the small GTPase Rab5 that mediates membrane fusion in endocytosis, is one such protein. We demonstrate that GAP-43 regulates endocytosis and synaptic vesicle recycling. Modulation of endocytosis by GAP-43, in association with rabaptin-5, may constitute a common molecular mechanism by which GAP-43 regulates membrane dynamics during its known roles in activity-dependent neurotransmitter release and neurite outgrowth.

Key words: GAP-43; growth-associated protein; calmodulin; rabaptin-5; endocytosis; synaptic vesicle

---

Copyright © 1998 Society for Neuroscience  0270-6474/98/18197757-11$05.00/0

This article has been cited by other articles:

				E. A. Kelly, M.-E. Tremblay, J. S. McCasland, and A. K. Majewska Postsynaptic Deregulation in GAP-43 Heterozygous Mouse Barrel Cortex Cereb Cortex, November 13, 2009; (2009) bhp231v1. [Abstract] [Full Text] [PDF]

				M. J. Albright, M. C. Weston, M. Inan, C. Rosenmund, and M. C. Crair Increased Thalamocortical Synaptic Response and Decreased Layer IV Innervation in GAP-43 Knockout Mice J Neurophysiol, September 1, 2007; 98(3): 1610 - 1625. [Abstract] [Full Text] [PDF]

				B. W. McColl, D. I. Graham, C. J. Weir, F. White, and K. Horsburgh Endocytic Pathway Alterations in Human Hippocampus after Global Ischemia and the Influence of APOE Genotype Am. J. Pathol., January 1, 2003; 162(1): 273 - 281. [Abstract] [Full Text] [PDF]

				S. L. Donovan, L. A. Mamounas, A. M. Andrews, M. E. Blue, and J. S. McCasland GAP-43 Is Critical for Normal Development of the Serotonergic Innervation in Forebrain J. Neurosci., May 1, 2002; 22(9): 3543 - 3552. [Abstract] [Full Text] [PDF]

				R. Lippe, M. Miaczynska, V. Rybin, A. Runge, and M. Zerial Functional Synergy between Rab5 Effector Rabaptin-5 and Exchange Factor Rabex-5 When Physically Associated in a Complex Mol. Biol. Cell, July 1, 2001; 12(7): 2219 - 2228. [Abstract] [Full Text] [PDF]

				A. Routtenberg, I. Cantallops, S. Zaffuto, P. Serrano, and U. Namgung Enhanced learning after genetic overexpression of a brain growth protein PNAS, June 20, 2000; 97(13): 7657 - 7662. [Abstract] [Full Text] [PDF]

				J. Y. Sung, J. Kim, S. R. Paik, J. H. Park, Y. S. Ahn, and K. C. Chung Induction of Neuronal Cell Death by Rab5A-dependent Endocytosis of alpha -Synuclein J. Biol. Chem., July 13, 2001; 276(29): 27441 - 27448. [Abstract] [Full Text] [PDF]

				S. Millecamps, D. Nicolle, I. Ceballos-Picot, J. Mallet, and M. Barkats Synaptic sprouting increases the uptake capacities of motoneurons in amyotrophic lateral sclerosis mice PNAS, June 19, 2001; 98(13): 7582 - 7587. [Abstract] [Full Text] [PDF]

				R. A. Johnson, A. J. Okragly, M. Haak-Frendscho, and G. S. Mitchell Cervical Dorsal Rhizotomy Increases Brain-Derived Neurotrophic Factor and Neurotrophin-3 Expression in the Ventral Spinal Cord J. Neurosci., May 15, 2000; 20(10): RC77 - RC77. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help