 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
The Journal of Neuroscience, November 1, 1998, 18(21):8770-8779
Presynaptic Induction and Expression of Homosynaptic Depression at Aplysia Sensorimotor Neuron Synapses
Beth A. Armitage and Steven A. Siegelbaum Center for Neurobiology and Behavior, Department of Pharmacology, Columbia University, Howard Hughes Medical Institute, New York, New York 10032
The cellular mechanisms underlying the induction and expression of homosynaptic depression at the glutamatergic synapse between Aplysia sensory and motor neurons were studied in dissociated cell culture. Intracellular microelectrodes were used to stimulate action potentials in the presynaptic sensory neuron and record the depolarizing EPSP from the motor neuron. Homosynaptic depression (HSD) was induced by repeatedly stimulating the sensory neuron at rates as low as one action potential per minute. Activation of postsynaptic Glu receptors was neither sufficient nor necessary to induce HSD. Thus, repeated applications of exogenous Glu did not depress the synaptically evoked EPSP. Moreover, normal HSD was observed when the sensory neuron was stimulated during a period when the Glu receptors were blocked with the antagonist DNQX. The induction of HSD is thus likely to occur within the presynaptic terminal. We explored the role of presynaptic calcium in the induction of HSD by injecting the sensory neuron with EGTA, a relatively slow calcium chelator that does not alter rapid release but effectively buffers the slow residual calcium transient thought to be important for plasticity. EGTA had little effect on HSD, indicating that residual Cai is not involved. HSD does not appear to involve a decrease in presynaptic calcium influx, because there was no change in the presynaptic calcium transient, measured by calcium indicator dyes, during HSD. We conclude that HSD is induced and expressed in the presynaptic terminal, possibly by a mechanism directly coupled to the release process.
Key words: synaptic transmission; synaptic plasticity; Glu receptors; presynaptic calcium; transmitter release; learning and memory
Copyright © 1998 Society for Neuroscience 0270-6474/98/18218770-10$05.00/0
This article has been cited by other articles:
D. Fioravante, R.-Y. Liu, A. K. Netek, L. J. Cleary, and J. H. Byrne
Synapsin Regulates Basal Synaptic Strength, Synaptic Depression, and Serotonin-Induced Facilitation of Sensorimotor Synapses in Aplysia
J Neurophysiol, December 1, 2007; 98(6): 3568 - 3580.
[Abstract] [Full Text] [PDF]
G. Houeland, A. Nakhost, W. S. Sossin, and V. F. Castellucci
PKC Modulation of Transmitter Release by SNAP-25 at Sensory-to-Motor Synapses in Aplysia
J Neurophysiol, January 1, 2007; 97(1): 134 - 143.
[Abstract] [Full Text] [PDF]
P. Lovell and L. L. Moroz
The largest growth cones in the animal kingdom: an illustrated guide to the dynamics of Aplysia neuronal growth in cell culture
Integr. Comp. Biol., December 1, 2006; 46(6): 847 - 870.
[Abstract] [Full Text] [PDF]
Y.-J. Sung, F. Wu, S. Schacher, and R. T. Ambron
Synaptogenesis regulates axotomy-induced activation of c-Jun-activator protein-1 transcription.
J. Neurosci., June 14, 2006; 26(24): 6439 - 6449.
[Abstract] [Full Text] [PDF]
D. L. Glanzman
The Cellular Mechanisms of Learning in Aplysia: Of Blind Men and Elephants
Biol. Bull., June 1, 2006; 210(3): 271 - 279.
[Abstract] [Full Text] [PDF]
Y. Wu, F. Kawasaki, and R. W. Ordway
Properties of Short-Term Synaptic Depression at Larval Neuromuscular Synapses in Wild-Type and Temperature-Sensitive Paralytic Mutants of Drosophila
J Neurophysiol, May 1, 2005; 93(5): 2396 - 2405.
[Abstract] [Full Text] [PDF]
O. Khabour, J. Levenson, L. C. Lyons, L. S. Kategaya, J. Chin, J. H. Byrne, and A. Eskin
Coregulation of Glutamate Uptake and Long-Term Sensitization in Aplysia
J. Neurosci., October 6, 2004; 24(40): 8829 - 8837.
[Abstract] [Full Text] [PDF]
Y. Ezzeddine and D. L. Glanzman
Prolonged Habituation of the Gill-Withdrawal Reflex in Aplysia Depends on Protein Synthesis, Protein Phosphatase Activity, and Postsynaptic Glutamate Receptors
J. Neurosci., October 22, 2003; 23(29): 9585 - 9594.
[Abstract] [Full Text] [PDF]
J. K. Rose, K. R. Kaun, S. H. Chen, and C. H. Rankin
GLR-1, a Non-NMDA Glutamate Receptor Homolog, Is Critical for Long-Term Memory in Caenorhabditis elegans
J. Neurosci., October 22, 2003; 23(29): 9595 - 9599.
[Abstract] [Full Text] [PDF]
E. G. Antzoulatos, L. J. Cleary, A. Eskin, D. A. Baxter, and J. H. Byrne
Desensitization of Postsynaptic Glutamate Receptors Contributes to High-Frequency Homosynaptic Depression of Aplysia Sensorimotor Connections
Learn. Mem., September 1, 2003; 10(5): 309 - 313.
[Abstract] [Full Text] [PDF]
Y. Zhao and M. Klein
Modulation of the Readily Releasable Pool of Transmitter and of Excitation-Secretion Coupling by Activity and by Serotonin at Aplysia Sensorimotor Synapses in Culture
J. Neurosci., December 15, 2002; 22(24): 10671 - 10679.
[Abstract] [Full Text] [PDF]
T. D. Gover, X.-Y. Jiang, and T. W. Abrams
Persistent, Exocytosis-Independent Silencing of Release Sites Underlies Homosynaptic Depression at Sensory Synapses in Aplysia
J. Neurosci., March 1, 2002; 22(5): 1942 - 1955.
[Abstract] [Full Text] [PDF]
A. S. Bristol, T. M. Fischer, and T. J. Carew
Combined Effects of Intrinsic Facilitation and Modulatory Inhibition of Identified Interneurons in the Siphon Withdrawal Circuitry of Aplysia
J. Neurosci., November 15, 2001; 21(22): 8990 - 9000.
[Abstract] [Full Text] [PDF]
R. A Chitwood, Q. Li, and D. L Glanzman
Serotonin facilitates AMPA-type responses in isolated siphon motor neurons of Aplysia in culture
J. Physiol., July 15, 2001; 534(2): 501 - 510.
[Abstract] [Full Text] [PDF]
A. L. Purcell and T. J. Carew
Modulation of Excitability in Aplysia Tail Sensory Neurons by Tyrosine Kinases
J Neurophysiol, June 1, 2001; 85(6): 2398 - 2411.
[Abstract] [Full Text] [PDF]
J. Levenson, S. Endo, L. S. Kategaya, R. I. Fernandez, D. G. Brabham, J. Chin, J. H. Byrne, and A. Eskin
Long-term regulation of neuronal high-affinity glutamate and glutamine uptake in Aplysia
PNAS, October 23, 2000; (2000) 220256497.
[Abstract] [Full Text]
J. Levenson, S. Endo, L. S. Kategaya, R. I. Fernandez, D. G. Brabham, J. Chin, J. H. Byrne, and A. Eskin
Long-term regulation of neuronal high-affinity glutamate and glutamine uptake in Aplysia
PNAS, November 7, 2000; 97(23): 12858 - 12863.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|