 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
The Journal of Neuroscience, December 1, 1998, 18(23):10136-10149
Immunocytochemical Localization of the Postsynaptic Density Protein PSD-95 in the Mammalian Retina
Peter Koulen1, Erica L. Fletcher1, Sarah E. Craven2, David S. Bredt2, and Heinz Wässle1 1 Max-Planck-Institut für Hirnforschung, D-60528 Frankfurt, Germany, and 2 Department of Physiology, University of California at San Francisco, San Francisco, California 94143
Synapse-associated proteins are the scaffold for the selective aggregation of ion channels at synapses; they provide the link to cytoskeletal elements and possibly are involved with the regulation of synaptic efficacy by electrical activity. The localization of the postsynaptic density protein PSD-95 was studied in different mammalian retinae (rat, monkey, and tree shrew) by using immunocytochemical methods. Immunofluorescence for PSD-95 was most prominent in the outer plexiform layer (OPL). The axon terminals of rods and cones, the rod spherules and cone pedicles, were strongly labeled. Electron microscopy, using preembedding immunocytochemistry, showed PSD-95 localized presynaptically within the photoreceptor terminals. Distinct PSD-95 labeling was also present in the inner plexiform layer (IPL). It had a punctate appearance suggesting the synaptic clustering of PSD-95 in the IPL. Electron microscopy showed that PSD-95 was concentrated in processes that were postsynaptic at bipolar cell ribbon synapses (dyads). As a rule, only one of the two postsynaptic members of the dyad was labeled for PSD-95. Double-labeling experiments were performed for PSD-95 and for SAP 102 or PSD-93, respectively, two other members of the family of synapse-associated proteins. All three were found to be colocalized in the synaptic hot spots in the IPL. In the OPL, however, PSD-95 and PSD-93 were found presynaptically, whereas SAP 102 was located postsynaptically at photoreceptor synapses. Double-labeling experiments also were performed for PSD-95 and for the NR1 subunit of the NMDA receptor. They were found to be colocalized in synaptic hot spots in the IPL.
Key words: PSD-95; SAP 102; PSD-93; NMDA receptors; NR1 subunit; postsynaptic density; receptor clustering; cone pedicle; rod spherule
Copyright © 1998 Society for Neuroscience 0270-6474/98/182310136-14$05.00/0
This article has been cited by other articles:
M. Yamagata and J. R. Sanes
Synaptic Localization and Function of Sidekick Recognition Molecules Require MAGI Scaffolding Proteins
J. Neurosci., March 10, 2010; 30(10): 3579 - 3588.
[Abstract] [Full Text] [PDF]
X. Li, W. Ma, Y. Zhuo, R.-T. Yan, and S.-Z. Wang
Using neurogenin to Reprogram Chick RPE to Produce Photoreceptor-like Neurons
Invest. Ophthalmol. Vis. Sci., January 1, 2010; 51(1): 516 - 525.
[Abstract] [Full Text] [PDF]
H. Stohr, J. B. Heisig, P. M. Benz, S. Schoberl, V. M. Milenkovic, O. Strauss, W. M. Aartsen, J. Wijnholds, B. H. F. Weber, and H. L. Schulz
TMEM16B, A Novel Protein with Calcium-Dependent Chloride Channel Activity, Associates with a Presynaptic Protein Complex in Photoreceptor Terminals
J. Neurosci., May 27, 2009; 29(21): 6809 - 6818.
[Abstract] [Full Text] [PDF]
C. Puller, L. P. de Sevilla Muller, U. Janssen-Bienhold, and S. Haverkamp
ZO-1 and the Spatial Organization of Gap Junctions and Glutamate Receptors in the Outer Plexiform Layer of the Mammalian Retina
J. Neurosci., May 13, 2009; 29(19): 6266 - 6275.
[Abstract] [Full Text] [PDF]
J. Zhang and J. S. Diamond
Subunit- and Pathway-Specific Localization of NMDA Receptors and Scaffolding Proteins at Ganglion Cell Synapses in Rat Retina
J. Neurosci., April 1, 2009; 29(13): 4274 - 4286.
[Abstract] [Full Text] [PDF]
J. L. Lefebvre, Y. Zhang, M. Meister, X. Wang, and J. R. Sanes
{gamma}-Protocadherins regulate neuronal survival but are dispensable for circuit formation in retina
Development, December 15, 2008; 135(24): 4141 - 4151.
[Abstract] [Full Text] [PDF]
N. A. Dmitrieva, C. E. Strang, and K. T. Keyser
Expression of Alpha 7 Nicotinic Acetylcholine Receptors by Bipolar, Amacrine, and Ganglion Cells of the Rabbit Retina
J. Histochem. Cytochem., May 1, 2007; 55(5): 461 - 476.
[Abstract] [Full Text] [PDF]
S. Kurumada, A. Onishi, H. Imai, K. Ishii, T. Kobayashi, and S. B. Sato
Stage-Specific Association of Apolipoprotein A-I and E in Developing Mouse Retina
Invest. Ophthalmol. Vis. Sci., April 1, 2007; 48(4): 1815 - 1823.
[Abstract] [Full Text] [PDF]
L. C. Liets, K. Eliasieh, D. A. van der List, and L. M. Chalupa
Dendrites of rod bipolar cells sprout in normal aging retina
PNAS, August 8, 2006; 103(32): 12156 - 12160.
[Abstract] [Full Text] [PDF]
W. M. Aartsen, A. Kantardzhieva, J. Klooster, A. G.S.H. van Rossum, S. A. van de Pavert, I. Versteeg, B. N. Cardozo, F. Tonagel, S. C. Beck, N. Tanimoto, et al.
Mpp4 recruits Psd95 and Veli3 towards the photoreceptor synapse
Hum. Mol. Genet., April 15, 2006; 15(8): 1291 - 1302.
[Abstract] [Full Text] [PDF]
R. G. Zhai and H. J. Bellen
The Architecture of the Active Zone in the Presynaptic Nerve Terminal
Physiology, October 1, 2004; 19(5): 262 - 270.
[Abstract] [Full Text] [PDF]
Y. Takada, R. N. Fariss, A. Tanikawa, Y. Zeng, D. Carper, R. Bush, and P. A. Sieving
A Retinal Neuronal Developmental Wave of Retinoschisin Expression Begins in Ganglion Cells during Layer Formation
Invest. Ophthalmol. Vis. Sci., September 1, 2004; 45(9): 3302 - 3312.
[Abstract] [Full Text] [PDF]
S. Seeber, A. Humeny, M. Herkert, T. Rau, T. Eschenhagen, and C.-M. Becker
Formation of Molecular Complexes by N-Methyl-D-aspartate Receptor Subunit NR2B and Ryanodine Receptor 2 in Neonatal Rat Myocard
J. Biol. Chem., May 14, 2004; 279(20): 21062 - 21068.
[Abstract] [Full Text] [PDF]
H. Stohr, J. Stojic, and B. H. F. Weber
Cellular Localization of the MPP4 Protein in the Mammalian Retina
Invest. Ophthalmol. Vis. Sci., December 1, 2003; 44(12): 5067 - 5074.
[Abstract] [Full Text] [PDF]
M. Ishii, A. Fujita, K. Iwai, S. Kusaka, K. Higashi, A. Inanobe, H. Hibino, and Y. Kurachi
Differential expression and distribution of Kir5.1 and Kir4.1 inwardly rectifying K+ channels in retina
Am J Physiol Cell Physiol, August 1, 2003; 285(2): C260 - C267.
[Abstract] [Full Text] [PDF]
S. Kaja, S.-H. Yang, J. Wei, K. Fujitani, R. Liu, A.-M. Brun-Zinkernagel, J. W. Simpkins, K. Inokuchi, and P. Koulen
Estrogen Protects the Inner Retina from Apoptosis and Ischemia-Induced Loss of Vesl-1L/Homer 1c Immunoreactive Synaptic Connections
Invest. Ophthalmol. Vis. Sci., July 1, 2003; 44(7): 3155 - 3162.
[Abstract] [Full Text] [PDF]
J. Johnson, N. Tian, M. S. Caywood, R. J. Reimer, R. H. Edwards, and D. R. Copenhagen
Vesicular Neurotransmitter Transporter Expression in Developing Postnatal Rodent Retina: GABA and Glycine Precede Glutamate
J. Neurosci., January 15, 2003; 23(2): 518 - 529.
[Abstract] [Full Text] [PDF]
H. Mok, H. Shin, S. Kim, J.-R. Lee, J. Yoon, and E. Kim
Association of the Kinesin Superfamily Motor Protein KIF1Balpha with Postsynaptic Density-95 (PSD-95), Synapse-Associated Protein-97, and Synaptic Scaffolding Molecule PSD-95/Discs Large/Zona Occludens-1 Proteins
J. Neurosci., July 1, 2002; 22(13): 5253 - 5258.
[Abstract] [Full Text] [PDF]
B. H. F. Weber, H. Schrewe, L. L. Molday, A. Gehrig, K. L. White, M. W. Seeliger, G. B. Jaissle, C. Friedburg, E. Tamm, and R. S. Molday
Inactivation of the murine X-linked juvenile retinoschisis gene, Rs1h, suggests a role of retinoschisin in retinal cell layer organization and synaptic structure
PNAS, April 30, 2002; 99(9): 6222 - 6227.
[Abstract] [Full Text] [PDF]
S. Chen and J. S. Diamond
Synaptically Released Glutamate Activates Extrasynaptic NMDA Receptors on Cells in the Ganglion Cell Layer of Rat Retina
J. Neurosci., March 15, 2002; 22(6): 2165 - 2173.
[Abstract] [Full Text] [PDF]
K. K. Ghosh, S. Haverkamp, and H. Wassle
Glutamate Receptors in the Rod Pathway of the Mammalian Retina
J. Neurosci., November 1, 2001; 21(21): 8636 - 8647.
[Abstract] [Full Text] [PDF]
T. Q. Vu, J. A. Payne, and D. R. Copenhagen
Localization and Developmental Expression Patterns of the Neuronal K-Cl Cotransporter (KCC2) in the Rat Retina
J. Neurosci., February 15, 2000; 20(4): 1414 - 1423.
[Abstract] [Full Text] [PDF]
N. Sans, R. S. Petralia, Y.-X. Wang, J. Blahos II, J. W. Hell, and R. J. Wenthold
A Developmental Change in NMDA Receptor-Associated Proteins at Hippocampal Synapses
J. Neurosci., February 1, 2000; 20(3): 1260 - 1271.
[Abstract] [Full Text] [PDF]
T Claudepierre, C Dalloz, D Mornet, K Matsumura, J Sahel, and A Rendon
Characterization of the intermolecular associations of the dystrophin-associated glycoprotein complex in retinal Muller glial cells
J. Cell Sci., January 10, 2000; 113(19): 3409 - 3417.
[Abstract] [PDF]
|
|
|
|
 |
|