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The Journal of Neuroscience, December 15, 1998, 18(24):10398-10408

Heteromultimeric Delayed-Rectifier K⁺ Channels in Schwann Cells: Developmental Expression and Role in Cell Proliferation

Alexander Sobko¹, Asher Peretz¹, Orian Shirihai², Sarah Etkin¹, Vera Cherepanova¹, Daniel Dagan², and Bernard Attali¹

¹ Neurobiology Department, Weizmann Institute of Science, Rehovot 76100, Israel, and ² Bruce Rappaport Faculty of Medicine, Bernard Katz Minerva Center for Cell Biophysics, Technion, Haifa 31096, Israel

Schwann cells (SCs) are responsible for myelination of nerve fibers in the peripheral nervous system. Voltage-dependent K⁺ currents, including inactivating A-type (K_A), delayed-rectifier (K_D), and inward-rectifier (K_IR) K⁺ channels, constitute the main conductances found in SCs. Physiological studies have shown that K_D channels may play an important role in SC proliferation and that they are downregulated in the soma as proliferation ceases and myelination proceeds. Recent studies have begun to address the molecular identity of K⁺ channels in SCs. Here, we show that a large repertoire of K⁺ channel  subunits of the Shaker (Kv1.1, Kv1.2, Kv1.4, and Kv1.5), Shab (Kv2.1), and Shaw (Kv3.1b and Kv3.2) families is expressed in mouse SCs and sciatic nerve. We characterized heteromultimeric channel complexes that consist of either Kv1.5 and Kv1.2 or Kv1.5 and Kv1.4. In postnatal day 4 (P4) sciatic nerve, most of the Kv1.2 channel subunits are involved in heteromultimeric association with Kv1.5. Despite the presence of Kv1.1 and Kv1.2  subunits, the K⁺ currents were unaffected by dendrotoxin I (DTX), suggesting that DTX-sensitive channel complexes do not account substantially for SC K_D currents. SC proliferation was found to be potently blocked by quinidine or 4-aminopyridine but not by DTX. Consistent with previous physiological studies, our data show that there is a marked downregulation of all K_D channel  subunits from P1-P4 to P40 in the sciatic nerve. Our results suggest that K_D currents are accounted for by a complex combinatorial activity of distinct K⁺ channel complexes and confirm that K_D channels are involved in SC proliferation.

Key words: K⁺ channels; Schwann cells; myelination; proliferation; development; ion channels; heteromultimeric association

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Copyright © 1998 Society for Neuroscience  0270-6474/98/182410398-11$05.00/0

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