QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (85) Citing Articles via Google Scholar Google Scholar Articles by Smith, R. D. Articles by Goldin, A. L. Search for Related Content PubMed PubMed Citation Articles by Smith, R. D. Articles by Goldin, A. L.

 Previous Article  |  Next Article 

The Journal of Neuroscience, February 1, 1998, 18(3):811-820

Functional Analysis of the Rat I Sodium Channel in Xenopus Oocytes

Raymond D. Smith and Alan L. Goldin

Department of Microbiology and Molecular Genetics, University of California, Irvine, California 92697-4025

Voltage-gated sodium channels in the mammalian CNS initiate and propagate action potentials when excitatory inputs achieve threshold membrane depolarization. There are multiple sodium channel isoforms expressed in rat brain (types I, II, III, 6, and NaG). We have constructed a full-length cDNA clone encoding type I and compared the electrophysiological properties of type I (Rat1) and II (Rat2) channels in the absence and presence of the two accessory subunits ₁ and ₂. Injection into Xenopus oocytes of RNA encoding Rat1 resulted in functional sodium currents that were blocked by tetrodotoxin, with K_app = 9.6 nM. Rat1 sodium channels had a slower time course of fast inactivation than Rat2. Coexpression of ₁ accelerated inactivation of both Rat1 and Rat2, resulting in comparable inactivation kinetics. Rat1 recovered from fast inactivation more rapidly than Rat2, regardless of whether ₁ or ₂ was present. The voltage dependence of activation was similar for Rat1 and Rat2 without the  subunits, but it was more positive for Rat1 when ₁ and ₂ were coexpressed. The voltage dependence of inactivation was more positive for Rat1 than for Rat2, and coexpression with ₁ and ₂ accentuated that difference. Finally, sodium current amplitudes were reduced by 7-9% for both Rat1 and Rat2 channels when protein kinase A phosphorylation was induced. It has been suggested previously that Rat1 and Rat6 channels mediate transient and maintained sodium conductances, respectively, in Purkinje cells, and the electrophysiological properties of Rat1 currents are consistent with a role for this channel in mediating the rapidly inactivating, transient current.

Key words: sodium channel; cloning; expression; Xenopus oocytes; brain; RT-PCR; protein kinase A; Purkinje cells

---

Copyright © 1998 Society for Neuroscience  0270-6474/98/183811-10$05.00/0

This article has been cited by other articles:

				G. A. Patino, L. R. F. Claes, L. F. Lopez-Santiago, E. A. Slat, R. S. R. Dondeti, C. Chen, H. A. O'Malley, C. B. B. Gray, H. Miyazaki, N. Nukina, et al. A Functional Null Mutation of SCN1B in a Patient with Dravet Syndrome J. Neurosci., August 26, 2009; 29(34): 10764 - 10778. [Abstract] [Full Text] [PDF]

				A. Lee and A. L. Goldin Role of the amino and carboxy termini in isoform-specific sodium channel variation J. Physiol., August 15, 2008; 586(16): 3917 - 3926. [Abstract] [Full Text] [PDF]

				W. P. Few, T. Scheuer, and W. A. Catterall Dopamine modulation of neuronal Na+ channels requires binding of A kinase-anchoring protein 15and PKA by a modified leucine zipper motif PNAS, March 20, 2007; 104(12): 5187 - 5192. [Abstract] [Full Text] [PDF]

				J. R. A. Wooltorton, S. Gaboyard, K. M. Hurley, S. D. Price, J. L. Garcia, M. Zhong, A. Lysakowski, and R. A. Eatock Developmental Changes in Two Voltage-Dependent Sodium Currents in Utricular Hair Cells J Neurophysiol, February 1, 2007; 97(2): 1684 - 1704. [Abstract] [Full Text] [PDF]

				F. Bosmans, L. Rash, S. Zhu, S. Diochot, M. Lazdunski, P. Escoubas, and J. Tytgat Four Novel Tarantula Toxins as Selective Modulators of Voltage-Gated Sodium Channel Subtypes Mol. Pharmacol., February 1, 2006; 69(2): 419 - 429. [Abstract] [Full Text] [PDF]

				M. Pertin, R.-R. Ji, T. Berta, A. J. Powell, L. Karchewski, S. N. Tate, L. L. Isom, C. J. Woolf, N. Gilliard, D. R. Spahn, et al. Upregulation of the Voltage-Gated Sodium Channel {beta}2 Subunit in Neuropathic Pain Models: Characterization of Expression in Injured and Non-Injured Primary Sensory Neurons J. Neurosci., November 23, 2005; 25(47): 10970 - 10980. [Abstract] [Full Text] [PDF]

				E. K. Wittmack, A. M. Rush, A. Hudmon, S. G. Waxman, and S. D. Dib-Hajj Voltage-Gated Sodium Channel Nav1.6 Is Modulated by p38 Mitogen-Activated Protein Kinase J. Neurosci., July 13, 2005; 25(28): 6621 - 6630. [Abstract] [Full Text] [PDF]

				N. Wu, A. Enomoto, S. Tanaka, C.-F. Hsiao, D. Q. Nykamp, E. Izhikevich, and S. H. Chandler Persistent Sodium Currents in Mesencephalic V Neurons Participate in Burst Generation and Control of Membrane Excitability J Neurophysiol, May 1, 2005; 93(5): 2710 - 2722. [Abstract] [Full Text] [PDF]

				V. Haufe, J. A. Camacho, R. Dumaine, B. Gunther, C. Bollensdorff, G. S. von Banchet, K. Benndorf, and T. Zimmer Expression pattern of neuronal and skeletal muscle voltage-gated Na+ channels in the developing mouse heart J. Physiol., May 1, 2005; 564(3): 683 - 696. [Abstract] [Full Text] [PDF]

				M. Mantegazza, F. H. Yu, A. J. Powell, J. J. Clare, W. A. Catterall, and T. Scheuer Molecular Determinants for Modulation of Persistent Sodium Current by G-Protein {beta}{gamma} Subunits J. Neurosci., March 30, 2005; 25(13): 3341 - 3349. [Abstract] [Full Text] [PDF]

				J. Spampanato, J. A. Kearney, G. de Haan, D. P. McEwen, A. Escayg, I. Aradi, B. T. MacDonald, S. I. Levin, I. Soltesz, P. Benna, et al. A Novel Epilepsy Mutation in the Sodium Channel SCN1A Identifies a Cytoplasmic Domain for {beta} Subunit Interaction J. Neurosci., November 3, 2004; 24(44): 10022 - 10034. [Abstract] [Full Text] [PDF]

				D. Johnson, M. L. Montpetit, P. J. Stocker, and E. S. Bennett The Sialic Acid Component of the {beta}1 Subunit Modulates Voltage-gated Sodium Channel Function J. Biol. Chem., October 22, 2004; 279(43): 44303 - 44310. [Abstract] [Full Text] [PDF]

				M. Lei, S. A. Jones, J. Liu, M. K. Lancaster, S. S.-M. Fung, H. Dobrzynski, P. Camelliti, S. K. G. Maier, D. Noble, and M. R. Boyett Requirement of neuronal- and cardiac-type sodium channels for murine sinoatrial node pacemaking J. Physiol., September 15, 2004; 559(3): 835 - 848. [Abstract] [Full Text] [PDF]

				K. Vijayaragavan, M. Boutjdir, and M. Chahine Modulation of Nav1.7 and Nav1.8 Peripheral Nerve Sodium Channels by Protein Kinase A and Protein Kinase C J Neurophysiol, April 1, 2004; 91(4): 1556 - 1569. [Abstract] [Full Text] [PDF]

				T. M. Grieco and I. M. Raman Production of Resurgent Current in NaV1.6-Null Purkinje Neurons by Slowing Sodium Channel Inactivation with {beta}-Pompilidotoxin J. Neurosci., January 7, 2004; 24(1): 35 - 42. [Abstract] [Full Text] [PDF]

				R. I Herzog, T. R Cummins, F. Ghassemi, S. D Dib-Hajj, and S. G Waxman Distinct repriming and closed-state inactivation kinetics of Nav1.6 and Nav1.7 sodium channels in mouse spinal sensory neurons J. Physiol., September 15, 2003; 551(3): 741 - 750. [Abstract] [Full Text] [PDF]

				S. K. G. Maier, R. E. Westenbroek, T. T. Yamanushi, H. Dobrzynski, M. R. Boyett, W. A. Catterall, and T. Scheuer An unexpected requirement for brain-type sodium channels for control of heart rate in the mouse sinoatrial node PNAS, March 18, 2003; 100(6): 3507 - 3512. [Abstract] [Full Text] [PDF]

				R. A. Li, I. L. Ennis, T. Xue, H. M. Nguyen, G. F. Tomaselli, A. L. Goldin, and E. Marban Molecular Basis of Isoform-specific {micro}-Conotoxin Block of Cardiac, Skeletal Muscle, and Brain Na+ Channels J. Biol. Chem., February 28, 2003; 278(10): 8717 - 8724. [Abstract] [Full Text] [PDF]

				T. R. Cummins, S. D. Dib-Hajj, S. G. Waxman, and D. F. Donnelly Characterization and Developmental Changes of Na+ Currents of Petrosal Neurons With Projections to the Carotid Body J Neurophysiol, December 1, 2002; 88(6): 2993 - 3002. [Abstract] [Full Text] [PDF]

				T. Zimmer and K. Benndorf The Human Heart and Rat Brain IIA Na+ Channels Interact with Different Molecular Regions of the {beta}1 Subunit J. Gen. Physiol., November 25, 2002; 120(6): 887 - 895. [Abstract] [Full Text] [PDF]

				T. M. Grieco, F. S. Afshari, and I. M. Raman A Role for Phosphorylation in the Maintenance of Resurgent Sodium Current in Cerebellar Purkinje Neurons J. Neurosci., April 15, 2002; 22(8): 3100 - 3107. [Abstract] [Full Text] [PDF]

				S. K. G. Maier, R. E. Westenbroek, K. A. Schenkman, E. O. Feigl, T. Scheuer, and W. A. Catterall An unexpected role for brain-type sodium channels in coupling of cell surface depolarization to contraction in the heart PNAS, March 19, 2002; 99(6): 4073 - 4078. [Abstract] [Full Text] [PDF]

				J. Spampanato, A. Escayg, M. H. Meisler, and A. L. Goldin Functional Effects of Two Voltage-Gated Sodium Channel Mutations That Cause Generalized Epilepsy with Febrile Seizures Plus Type 2 J. Neurosci., October 1, 2001; 21(19): 7481 - 7490. [Abstract] [Full Text] [PDF]

				T. R. Cummins, F. Aglieco, M. Renganathan, R. I. Herzog, S. D. Dib-Hajj, and S. G. Waxman Nav1.3 Sodium Channels: Rapid Repriming and Slow Closed-State Inactivation Display Quantitative Differences after Expression in a Mammalian Cell Line and in Spinal Sensory Neurons J. Neurosci., August 15, 2001; 21(16): 5952 - 5961. [Abstract] [Full Text] [PDF]

				B S Shah, E B Stevens, R D Pinnock, A K Dixon, and K Lee Developmental expression of the novel voltage-gated sodium channel auxiliary subunit {beta}3, in rat CNS J. Physiol., August 1, 2001; 534(3): 763 - 776. [Abstract] [Full Text] [PDF]

				N. Maurice, T. Tkatch, M. Meisler, L. K. Sprunger, and D. J. Surmeier D1/D5 Dopamine Receptor Activation Differentially Modulates Rapidly Inactivating and Persistent Sodium Currents in Prefrontal Cortex Pyramidal Neurons J. Neurosci., April 1, 2001; 21(7): 2268 - 2277. [Abstract] [Full Text] [PDF]

				J. D. Malhotra, C. Chen, I. Rivolta, H. Abriel, R. Malhotra, L. N. Mattei, F. C. Brosius, R. S. Kass, and L. L. Isom Characterization of Sodium Channel {{alpha}}- and {beta}-Subunits in Rat and Mouse Cardiac Myocytes Circulation, March 6, 2001; 103(9): 1303 - 1310. [Abstract] [Full Text] [PDF]

				A. K Alekov, M. M. Rahman, N. Mitrovic, F. Lehmann-Horn, and H. Lerche A sodium channel mutation causing epilepsy in man exhibits subtle defects in fast inactivation and activation in vitro J. Physiol., December 15, 2000; 529(3): 533 - 539. [Abstract] [Full Text] [PDF]

				M. Baruscotti, D. DiFrancesco, and R. B. Robinson Na+ current contribution to the diastolic depolarization in newborn rabbit SA node cells Am J Physiol Heart Circ Physiol, November 1, 2000; 279(5): H2303 - H2309. [Abstract] [Full Text] [PDF]

				L. L. Isom Pathobiology of Visceral Pain: Molecular Mechanisms and Theraputic Implications. I. Cellular and molecular biology of sodium channel beta -subunits: therapeutic implications for pain? Am J Physiol Gastrointest Liver Physiol, March 1, 2000; 278(3): G349 - G353. [Abstract] [Full Text] [PDF]

				K. A. Kazen-Gillespie, D. S. Ragsdale, M. R. D'Andrea, L. N. Mattei, K. E. Rogers, and L. L. Isom Cloning, Localization, and Functional Expression of Sodium Channel beta 1A Subunits J. Biol. Chem., January 14, 2000; 275(2): 1079 - 1088. [Abstract] [Full Text] [PDF]

				J. Magistretti, D. S. Ragsdale, and A. Alonso High Conductance Sustained Single-Channel Activity Responsible for the Low-Threshold Persistent Na+ Current in Entorhinal Cortex Neurons J. Neurosci., September 1, 1999; 19(17): 7334 - 7341. [Abstract] [Full Text] [PDF]

				T. R. Cummins, J. R. Howe, and S. G. Waxman Slow Closed-State Inactivation: A Novel Mechanism Underlying Ramp Currents in Cells Expressing the hNE/PN1 Sodium Channel J. Neurosci., December 1, 1998; 18(23): 9607 - 9619. [Abstract] [Full Text] [PDF]

				A. R. Kay, M. Sugimori, and R. Llinas Kinetic and Stochastic Properties of a Persistent Sodium Current in Mature Guinea Pig Cerebellar Purkinje Cells J Neurophysiol, September 1, 1998; 80(3): 1167 - 1179. [Abstract] [Full Text] [PDF]

				M. R. Smith, R. D. Smith, N. W. Plummer, M. H. Meisler, and A. L. Goldin Functional Analysis of the Mouse Scn8a Sodium Channel J. Neurosci., August 15, 1998; 18(16): 6093 - 6102. [Abstract] [Full Text] [PDF]

				S. D. Dib-Hajj, L. Tyrrell, J. A. Black, and S. G. Waxman NaN, a novel voltage-gated Na channel, is expressed preferentially in peripheral sensory neurons and down-regulated after axotomy PNAS, July 21, 1998; 95(15): 8963 - 8968. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help