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The Journal of Neuroscience, March 15, 1998, 18(6):2301-2308
Action Potentials Are Required for the Lateral Transmission of
Glycinergic Transient Inhibition in the Amphibian Retina
Paul B.
Cook1,
Peter D.
Lukasiewicz2, and
John S.
McReynolds1
1 Department of Physiology, University of Michigan, Ann
Arbor, Michigan 48109, and 2 Department of Ophthalmology
and Visual Science, Washington University, St. Louis, Missouri 63110
Transient lateral inhibition (TLI), the suppression of responses of
a ganglion cell to light stimuli in the receptive field center by
changes in illumination in the receptive field surround, was studied in
light-adapted mud puppy and tiger salamander retinas using both eyecup
and retinal slice preparations. In the eyecup, TLI was measured in
on-off ganglion cells as the ability of rotating, concentric windmill
patterns of 500-1200 µm inner diameter to suppress the response to a
small spot stimulus in the receptive field center. Both the suppression
of the spot response and the hyperpolarization produced in ganglion
cells by rotation of the windmill were blocked in the presence of 2 µM strychnine or 500 nM tetrodotoxin (TTX),
but not by 150 µM picrotoxin. In the slice preparation in
which GABA-mediated currents were blocked with picrotoxin, IPSCs
elicited by diffuse illumination were blocked by strychnine and
strongly reduced by TTX. The TTX-resistant component was probably
attributable to illumination of the receptive field center. TTX had a
much greater effect in reducing the glycinergic inhibition elicited by
laterally displaced stimulation versus nearby focal electrical
stimulation. Strychnine enhanced light-evoked excitatory currents in
ganglion cells, but this was not mimicked by TTX. The results suggest
that local glycinergic transient inhibition does not require action
potentials and is mediated by synapses onto both ganglion cell
dendrites and bipolar cell terminals. In contrast, the lateral spread
of this inhibition (at least over distances >250 µm) requires action
potentials and is mainly onto ganglion cell dendrites.
Key words:
retina; ganglion cell; receptive field; lateral
inhibition; glycine; action potentials
Copyright © 1998 Society for Neuroscience 0270-6474/98/1862301-08$05.00/0
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