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Previous Article | Next Article
The Journal of Neuroscience, April 1, 1998, 18(7):2520-2537
Astrocytic Gap Junctions Remain Open during Ischemic Conditions
Maria Luisa Cotrina, Jian Kang, Jane H-C Lin, Earl Bueno, Thomas W. Hansen, Lili He, Yulin Liu, and Maiken Nedergaard Departments of Cell Biology and Anatomy and Pathology, New York Medical College, Valhalla, New York 10595
Gap junctions are highly conductive channels that allow the direct transfer of intracellular messengers such as Ca2+ and inositol triphosphate (IP3) between interconnected cells. In brain, astrocytes are coupled extensively by gap junctions. We found here that gap junctions among astrocytes in acutely prepared brain slices as well as in culture remained open during ischemic conditions. Uncoupling first occurred after the terminal loss of plasma membrane integrity. Gap junctions therefore may link ischemic astrocytes in an evolving infarct with the surroundings. The free exchange of intracellular messengers between dying and potentially viable astrocytes might contribute to secondary expansion of ischemic lesions.
Key words: stroke; apoptosis; calcium; pH; IP3; cell culture; digital imaging
Copyright © 1998 Society for Neuroscience 0270-6474/98/1872520-18$05.00/0
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