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The Journal of Neuroscience, April 1, 1998, 18(7):2538-2549
Developmental Expression of the µ,  , and  Opioid Receptor
mRNAs in Mouse
Yanxin
Zhu,
Ming-Sing
Hsu, and
John E.
Pintar
Department of Neuroscience and Cell Biology, University of Medicine
and Dentistry of New Jersey Robert Wood Johnson Medical School,
Piscataway, New Jersey 08854
To characterize further the establishment of the opioid
system during prenatal mouse development, we have examined the spatial and temporal expression patterns of µ,  , and  opioid receptor mRNAs and find that the expression patterns of these mRNAs are distinct
at all ages. Within the embryo, is the first opioid receptor
expressed, with transcripts detected in the gut epithelium as early as
embryonic day 9.5 (E9.5). By E10.5, µ receptor expression is first
detected in the facial-vestibulocochlear preganglion complex, whereas
receptor mRNA is first detected at E12.5 in several peripheral
tissues, including the olfactory epithelium, heart, limb bud, and
tooth. In the brain, both µ and mRNAs are first detected at E11.5
in the basal ganglia and midbrain, respectively. During mid-gestation
and late gestation, the expression of both µ and receptors
extends to other brain regions that exhibit high expression in the
adult, including the medial habenula, hypothalamus, pons, and medulla
for µ and the basal ganglia, thalamus, hypothalamus, raphe, and
ventral tegmental area for . Thus by E17.5, many aspects of the
adult expression patterns of µ and receptors already have been
established. Compared with µ and , receptor mRNA expression in
the brain begins relatively late, and the expression levels remain very
low even at E19.5. In contrast to its late appearance in the brain,
however, is the first opioid receptor expressed in the dorsal root
ganglion, at E12.5, before its expression in the spinal cord begins at
E15.5. µ receptor is the first opioid receptor expressed in the
spinal cord, at E11.5. These results extend previous ligand-binding
data to significantly earlier ages and suggest that early developmental
events in both neural and non-neural tissues may be modulated by opioid
receptors. Several examples of possible autocrine and paracrine loops
of opioid peptide and receptor expression have been identified,
suggesting a role for these local circuits in developmental
processes.
Key words:
opioid receptor; in situ hybridization; ontogeny; development; embryo; CNS
Copyright © 1998 Society for Neuroscience 0270-6474/98/1872538-12$05.00/0
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