QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (35) Citing Articles via Google Scholar Google Scholar Articles by Moon, C. Articles by Ronnett, G. V. Search for Related Content PubMed PubMed Citation Articles by Moon, C. Articles by Ronnett, G. V.

 Previous Article  |  Next Article 

The Journal of Neuroscience, May 1, 1998, 18(9):3195-3205

Calcium-Sensitive Particulate Guanylyl Cyclase as a Modulator of cAMP in Olfactory Receptor Neurons

Cheil Moon¹, Parham Jaberi¹, Annie Otto-Bruc³, Wolfgang Baehr⁵, Krzysztof Palczewski^{3, 4}, and Gabriele V. Ronnett^{1, 2}

Departments of ¹ Neuroscience and ² Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, Departments of ³ Ophthalmology and ⁴ Pharmacology, School of Medicine, University of Washington, Seattle, Washington 98185, and ⁵ Moran Eye Center, University of Utah Health Science Center, Salt Lake City, Utah 84132

The second messengers cAMP and inositol-1,4,5-triphosphate have been implicated in olfaction in various species. The odorant-induced cGMP response was investigated using cilia preparations and olfactory primary cultures. Odorants cause a delayed and sustained elevation of cGMP. A component of this cGMP response is attributable to the activation of one of two kinetically distinct cilial receptor guanylyl cyclases by calcium and a guanylyl cyclase-activating protein (GCAP). cGMP thus formed serves to augment the cAMP signal in a cGMP-dependent protein kinase (PKG) manner by direct activation of adenylate cyclase. cAMP, in turn, activates cAMP-dependent protein kinase (PKA) to negatively regulate guanylyl cyclase, limiting the cGMP signal. These data demonstrate the existence of a regulatory loop in which cGMP can augment a cAMP signal, and in turn cAMP negatively regulates cGMP production via PKA. Thus, a small, localized, odorant-induced cAMP response may be amplified to modulate downstream transduction enzymes or transcriptional events.

Key words: olfaction; signal transduction; guanylyl cyclase; olfactory receptor neuron; cilia; protein kinase

---

Copyright © 1998 Society for Neuroscience  0270-6474/98/1893195-11$05.00/0

This article has been cited by other articles:

				S. J. Kleene The Electrochemical Basis of Odor Transduction in Vertebrate Olfactory Cilia Chem Senses, August 14, 2008; (2008) bjn048v1. [Abstract] [Full Text] [PDF]

				U. Mayer, N. Ungerer, D. Klimmeck, U. Warnken, M. Schnolzer, S. Frings, and F. Mohrlen Proteomic Analysis of a Membrane Preparation from Rat Olfactory Sensory Cilia Chem Senses, February 1, 2008; 33(2): 145 - 162. [Abstract] [Full Text] [PDF]

				A. Walz, P. Feinstein, M. Khan, and P. Mombaerts Axonal wiring of guanylate cyclase-D-expressing olfactory neurons is dependent on neuropilin 2 and semaphorin 3F Development, November 15, 2007; 134(22): 4063 - 4072. [Abstract] [Full Text] [PDF]

				I. Sokal, N. Li, C. S. Klug, S. Filipek, W. L. Hubbell, W. Baehr, and K. Palczewski Calcium-sensitive Regions of GCAP1 as Observed by Chemical Modifications, Fluorescence, and EPR Spectroscopies J. Biol. Chem., November 9, 2001; 276(46): 43361 - 43373. [Abstract] [Full Text] [PDF]

				J. E. Engel, X.-J. Xie, M. B. Sokolowski, and C.-F. Wu A cGMP-Dependent Protein Kinase Gene, foraging, Modifies Habituation-Like Response Decrement of the Giant Fiber Escape Circuit in Drosophila Learn. Mem., September 1, 2000; 7(5): 341 - 352. [Abstract] [Full Text]

				F. Zufall and T. Leinders-Zufall The Cellular and Molecular Basis of Odor Adaptation Chem Senses, August 1, 2000; 25(4): 473 - 481. [Abstract] [Full Text] [PDF]

				C. Moon, Y.-K. Sung, R. Reddy, and G. V. Ronnett Odorants induce the phosphorylation of the cAMP response element binding protein in olfactory receptor neurons PNAS, December 7, 1999; 96(25): 14605 - 14610. [Abstract] [Full Text] [PDF]

				P. J. Simpson, A. Nighorn, and D. B. Morton Identification of a Novel Guanylyl Cyclase That Is Related to Receptor Guanylyl Cyclases, but Lacks Extracellular and Transmembrane Domains J. Biol. Chem., February 12, 1999; 274(7): 4440 - 4446. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help