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The Journal of Neuroscience, January 1, 1999, 19(1):288-297
The Mammalian Staufen Protein Localizes to the Somatodendritic Domain of Cultured Hippocampal Neurons: Implications for Its Involvement in mRNA Transport
Michael A. Kiebler1, Indradeo Hemraj1, Paul Verkade1, Martin Köhrmann1, Puri Fortes2, 3, Rosa M. Marión3, Juan Ortín3, and Carlos G. Dotti1 European Molecular Biology Laboratory, 1 Cell Biology Programme and 2 Gene Expression Programme, 69012 Heidelberg, Germany, and 3 Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Científicas, Campus de Cantoblanco, 28049 Madrid, Spain
In hippocampal neurons, certain mRNAs have been found in dendrites (), and their localization and translation have been implicated in synaptic plasticity (). One attractive candidate to achieve transport of mRNAs into dendrites is Staufen (Stau), a double-stranded RNA-binding protein, which plays a pivotal role in mRNA transport, localization, and translation in Drosophila (). Using antibodies raised against a peptide located in the RNA-binding domain IIa and a polyclonal antibody raised against a recently cloned human Staufen homolog, we identify a 65 kDa rat homolog in cultured rat hippocampal neurons. In agreement with the exclusive somatodendritic localization of mRNAs in these cells, we find that Staufen is restricted to the same domain. By immunoelectron microscopy, we show enrichment of the mammalian homolog of Stau (mStau) in the vicinity of smooth endoplasmic reticulum and microtubules near synaptic contacts. Finally, the association of the mStau with neuronal mRNAs is suggested by the colocalization with ribonucleoprotein particles specifically in distal dendrites known to contain mRNA, ribosomes, and translation factors (). These results suggest a role for mStau in the polarized transport and localization of mRNAs in mammalian neurons.
Key words: mammalian Staufen; double-stranded mRNA-binding protein; hippocampal neurons; mRNA transport; ribonucleoprotein particles; SYTO14
Copyright © 1999 Society for Neuroscience 0270-6474/99/191288-10$05.00/0
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