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The Journal of Neuroscience, June 1, 1999, 19(11):4360-4369

Neuroprotection at Drosophila Synapses Conferred by Prior Heat Shock

Shanker Karunanithi¹, Jeffrey W. Barclay², R. Meldrum Robertson², Ian R. Brown³, and Harold L. Atwood¹

¹ Department of Physiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8, ² Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6, and ³ Life Sciences Division, Scarborough Campus, University of Toronto, Toronto, Ontario, Canada M1C 1A4

Synapses are critical sites of information transfer in the nervous system, and it is important that their functionality be maintained under stressful conditions to prevent communication breakdown. Here we show that synaptic transmission at the Drosophila larval neuromuscular junction is protected by prior exposure to heat shock that strongly induces expression of heat shock proteins, in particular hsp70. Using a macropatch electrode to record synaptic activity at individual, visualized boutons, we found that prior heat shock sustains synaptic performance at high test temperatures through pre- and postsynaptic alterations. After heat shock, nerve impulses release more quantal units at high temperatures and exhibit fewer failures of release (presynaptic modification), whereas the amplitude of quantal currents remains more constant than does that in nonheat-shocked controls (postsynaptic modification). The time course of these physiological changes is similar to that of elevated hsp70. Thus, stress-induced neuroprotective mechanisms maintain function at synapses by modifying their properties.

Key words: Drosophila; heat shock proteins; quanta; thermal stress; presynaptic; postsynaptic; neuromuscular

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Copyright © 1999 Society for Neuroscience  0270-6474/99/19114360-10$05.00/0

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