Erratum
for
Goodyear and Richardson, J. Neurosci. 19 (10) 3761-3772.
Previous Article
The Journal of Neuroscience, August 15, 1999, 19(16):7238-7239
ERRATUM
Erratum
In the article "The Ankle-Ink Antigen: an Epitope
Sensitive to Calcium Chelation Associated with the Hair-Cell Surface
and the Calycal Processes of Photoreceptors," by Richard Goodyear and
Guy Richardson, which appeared on pages 3761-3772 of the May 15, 1999 issue, a printer's error caused inadvertent artifacts in Figures 1, 7,
9, and 10. The figures and legends are reprinted.
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Figure 1.
Localization of mAb E40 binding sites in the inner
ear and the retina. a, a', Section of the
cochlear duct double-labeled with mAb E40 (a) and
phalloidin (a'). Within the cochlear duct, mAb E40 is
specific for the apical surface of the hair cells in the basilar
papilla. Scale bar, 100 µm. b, b',
High-magnification image of a cryosection from the utricular macula
double-labeled with mAb E40 (b) and phalloidin
(b'). In sectioned material, mAb E40 staining is
predominantly restricted to the base of the hair bundle.
c, A whole-mount preparation of the utricular macula
labeled with mAb E40. Although the majority of the labeling is
restricted to the base of each hair bundle, punctate staining can be
seen all the way up the stereocilia. Scale bar: b,
b', c, 10 µm. d,
d', Section of the retina immunolabeled with mAb E40
(d) and the corresponding phase-contrast image
(d'). A spot of label is associated with each
photoreceptor at a level close to that of the oil droplets in the
cones. Arrowheads in d' indicate oil
droplets. Scale bar, 20 µm. BP, Basilar papilla;
H, homogene cells; P, photoreceptors;
RPE, retinal pigment epithelium; TV,
tegmentum vasculosum.
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Figure 7.
Effects of BAPTA and subtilisin on mAb E40
(a, c, e) and anti-HCA
labeling (b, d, f)
in extrastriolar regions of utricular maculae. a,
b, Control samples incubated in HBHBSS for 60 min.
c, d, Samples incubated in 5 mM BAPTA for 60 min. e, f,
Samples incubated in 50 µg/ml subtilisin for 20 min. In control
maculae (a, b), both mAb E40
(a) and monoclonal anti-HCA antibody
(b) label hair bundles strongly. After 1 hr of
BAPTA treatment, mAb E40 antigen no longer stains the cell surface
(c), whereas the distribution of the hair-cell
antigen is unchanged (d), although the hair
bundles are splayed. After 20 min exposure to subtilisin, the mAb E40
staining can no longer detected (e), and only
traces of the HCA remain (f). Scale bar, 10 µm.
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Figure 9.
Temperature dependence of the effects of BAPTA
treatment on mAb E40 labeling in extrastriolar regions of the utricular
macula. a, b, Maculae stained with mAb
E40 after a 20 min incubation in HBHBSS (a) or 5 mM BAPTA (b) at room temperature.
c, d, Maculae stained with mAb E40 after
a 20 min incubation in HBHBSS (c) or 5 mM BAPTA (d) at 2°C. Although
staining is reduced in the tissue treated with BAPTA at 2°C compared
with the control tissue, labeling is much stronger than that seen after
BAPTA treatment at room temperature (b).
e, f, Maculae stained with mAb E40 after
a 4 hr incubation in HBHBSS (e) or 5 mM BAPTA (f) at 2°C. The degree of
labeling observed after BAPTA treatment for 4 hr at 2°C
(f) is similar to that seen after 20 min at 2°C
(d). Scale bar, 20 µm.
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Figure 10.
Recovery of mAb E40 labeling after
BAPTA-induced loss. a, Control macula stained with mAb
E40 after 20 min incubation in HBHBSS. b, BAPTA-treated
(5 mM, 20 min) macula stained with mAb E40. Note that only
very weak labeling can be detected. c, Control,
HBHBSS-treated macula after 20 hr in vitro stained with
mAb E40. d, BAPTA-treated (5 mM, 20 min)
macula stained with mAb E40 after 20 hr in vitro. mAb
E40 staining has reappeared but not to the same level as in the control
(b). Scale bar, 10 µm.
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Copyright © 1999 Society for Neuroscience 0270-6474/99/19167238-02$05.00/0
