QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (13) Citing Articles via Google Scholar Google Scholar Articles by Legay, C. Articles by Jasmin, B. J. Search for Related Content PubMed PubMed Citation Articles by Legay, C. Articles by Jasmin, B. J.

 Previous Article  |  Next Article 

The Journal of Neuroscience, October 1, 1999, 19(19):8252-8259

Stability and Secretion of Acetylcholinesterase Forms in Skeletal Muscle Cells

Claire Legay¹, Fawzi A. Mankal², Jean Massoulié¹, and Bernard J. Jasmin²

¹ Laboratoire de Neurobiologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique UMR 8544, Ecole Normale Supérieure, 75005 Paris, France, and ² Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada K1H 8M5

Muscle cells express a distinct splice variant of acetylcholinesterase (AChE_T), but the specific mechanisms governing this restricted expression remain unclear. In these cells, a fraction of AChE subunits is associated with a triple helical collagen, ColQ, each strand of which can recruit a tetramer of AChE_T. In the present study, we examined the expression of the various splice variants of AChE by transfection in the mouse C2C12 myogenic cells in vitro, as well as in vivo by injecting plasmid DNA directly into tibialis anterior muscles of mice and rats. Surprisingly, we found that transfection with an ACHE_H cDNA, generating a glycophosphatidylinositol-anchored enzyme species, produced much more activity than transfection with AChE_T cDNA in both C2C12 cells and in vivo. This indicates that the exclusive expression of AChE_T in mature muscle is governed by specific splicing. Interaction of AChE_T subunits with the complete collagen tail ColQ increased enzyme activity in cultured cells, as well as in muscle fibers in vivo. Truncated ColQ subunits, presenting more or less extensive C-terminal deletions, also increased AChE activity and secretion in C2C12 cells, although the triple helix could not form in the case of the larger deletion. This suggests that heteromeric associations are stabilized compared with isolated AChE_T subunits. Coinjections of AChE_T and ColQ resulted in the production and secretion of asymmetric forms, indicating that assembly, processing, and externalization of these molecules can occur outside the junctional region of muscle fibers and hence does not require the specialized junctional Golgi apparatus.

Key words: acetylcholinesterase; collagen tail; C2C12 muscle cells; skeletal muscle; alternative splicing; synaptic proteins; neuromuscular junctions

---

Copyright © 1999 Society for Neuroscience  0270-6474/99/19198252-08$05.00/0

This article has been cited by other articles:

				S. Camp, A. De Jaco, L. Zhang, M. Marquez, B. De La Torre, and P. Taylor Acetylcholinesterase Expression in Muscle Is Specifically Controlled by a Promoter-Selective Enhancesome in the First Intron J. Neurosci., March 5, 2008; 28(10): 2459 - 2470. [Abstract] [Full Text] [PDF]

				J. Deschenes-Furry, G. Belanger, J. Mwanjewe, J. A. Lunde, R. J. Parks, N. Perrone-Bizzozero, and B. J. Jasmin The RNA-binding Protein HuR Binds to Acetylcholinesterase Transcripts and Regulates Their Expression in Differentiating Skeletal Muscle Cells J. Biol. Chem., July 8, 2005; 280(27): 25361 - 25368. [Abstract] [Full Text] [PDF]

				H. H. C. Lee, R. C. Y. Choi, A. K. L. Ting, N. L. Siow, J. X. S. Jiang, J. Massoulie, and K. W. K. Tsim Transcriptional Regulation of Acetylcholinesterase-associated Collagen ColQ: DIFFERENTIAL EXPRESSION IN FAST AND SLOW TWITCH MUSCLE FIBERS IS DRIVEN BY DISTINCT PROMOTERS J. Biol. Chem., June 25, 2004; 279(26): 27098 - 27107. [Abstract] [Full Text] [PDF]

				A. Cartaud, L. Strochlic, M. Guerra, B. Blanchard, M. Lambergeon, E. Krejci, J. Cartaud, and C. Legay MuSK is required for anchoring acetylcholinesterase at the neuromuscular junction J. Cell Biol., May 24, 2004; 165(4): 505 - 515. [Abstract] [Full Text] [PDF]

				R. C. Y. Choi, N. L. Siow, A. W. M. Cheng, K. K. Y. Ling, E. K. K. Tung, J. Simon, E. A. Barnard, and K. W. K. Tsim ATP Acts via P2Y1 Receptors to Stimulate Acetylcholinesterase and Acetylcholine Receptor Expression: Transduction and Transcription Control J. Neurosci., June 1, 2003; 23(11): 4445 - 4456. [Abstract] [Full Text] [PDF]

				M. Bendayan and V. Gisiger Demonstration of Acetylcholinesterase Molecular Forms in a Continuous Tubular Lysosomal System of Rat Pancreatic Acinar Cells J. Histochem. Cytochem., January 1, 2001; 49(1): 29 - 40. [Abstract] [Full Text]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help