QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (74) Citing Articles via Google Scholar Google Scholar Articles by El Idrissi, A. Articles by Trenkner, E. Search for Related Content PubMed PubMed Citation Articles by El Idrissi, A. Articles by Trenkner, E.

 Previous Article  |  Next Article 

The Journal of Neuroscience, November 1, 1999, 19(21):9459-9468

Growth Factors and Taurine Protect against Excitotoxicity by Stabilizing Calcium Homeostasis and Energy Metabolism

Abdeslem El Idrissi and Ekkhart Trenkner

New York State Institute for Basic Research in Developmental Disabilities, Center for Developmental Neuroscience, The Graduate School and University Center of the City University of New York, Staten Island, New York 10314

Taurine, brain derived neurotrophic factor (BDNF), and basic fibroblast growth factor (bFGF) are known to control the development of early postnatal cerebellar granule cells. This study attempted to investigate possible mechanisms of this control by determining neuronal survival, calcium homeostasis, and related calcium-mediated functions, as well as the site of action during glutamate-induced excitotoxicity in cultures of cerebellar granule cells. We report that stimulation of glutamate receptors induced a rapid increase in intracellular calcium concentrations ([Ca²⁺]_i) and a decrease in mitochondrial energy metabolism. These effects of glutamate were time- and concentration-dependent and could be specifically blocked by glutamate receptor antagonists. Taurine and bFGF but not BDNF differently regulated [Ca²⁺]_i, and preserved the mitochondrial energy metabolism in the presence of glutamate. The regulation of [Ca²⁺]_i by bFGF and taurine required pretreatment of cells with these factors. Confocal microscope analysis of [Ca²⁺]_i and ⁴⁵Ca²⁺ uptake studies showed that bFGF reduced the magnitude of glutamate-induced calcium uptake with no apparent regulation thereafter. Taurine, on the other hand, did not affect the level of calcium uptake induced by glutamate but rather the duration of the maximal response; this maximal response was transient and returned to basal levels ~10 min after glutamate receptor stimulation. We conclude from these data that bFGF and taurine prevent glutamate excitotoxicity through regulation of [Ca²⁺]_i and mitochondrial energy metabolism. Furthermore, the neuroprotective role of taurine and bFGF was enhanced by their collaboration.

Key words: cerebellar granule cells; excitotoxicity; growth factors; taurine; glutamate; calcium; energy metabolism; BDNF; bFGF

---

Copyright © 1999 Society for Neuroscience  0270-6474/99/19219459-10$05.00/0

This article has been cited by other articles:

				Y. Zhu, J. M. Antony, J. A. Martinez, D. M. Glerum, V. Brussee, A. Hoke, D. Zochodne, and C. Power Didanosine causes sensory neuropathy in an HIV/AIDS animal model: impaired mitochondrial and neurotrophic factor gene expression Brain, August 1, 2007; 130(8): 2011 - 2023. [Abstract] [Full Text] [PDF]

				I. Morales, J. G. Dopico, M. Sabate, T. Gonzalez-Hernandez, and M. Rodriguez Substantia nigra osmoregulation: taurine and ATP involvement Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1934 - C1941. [Abstract] [Full Text] [PDF]

				S. Studenski, M. C. Carlson, H. Fillit, W. T. Greenough, A. Kramer, and G. W. Rebok From Bedside to Bench: Does Mental and Physical Activity Promote Cognitive Vitality in Late Life? Sci. Aging Knowl. Environ., June 28, 2006; 2006(10): pe21 - pe21. [Abstract] [Full Text]

				S. Vaynman and F. Gomez-Pinilla License to Run: Exercise Impacts Functional Plasticity in the Intact and Injured Central Nervous System by Using Neurotrophins Neurorehabil Neural Repair, December 1, 2005; 19(4): 283 - 295. [Abstract] [PDF]

				F. Li, I. G. Obrosova, O. Abatan, D. Tian, D. Larkin, E. L. Stuenkel, and M. J. Stevens Taurine replacement attenuates hyperalgesia and abnormal calcium signaling in sensory neurons of STZ-D rats Am J Physiol Endocrinol Metab, January 1, 2005; 288(1): E29 - E36. [Abstract] [Full Text] [PDF]

				P. R. LOUZADA, A. C. P. LIMA, D. L. MENDONCA-SILVA, F. NOEL, F. G. DE MELLO, and S. T. FERREIRA Taurine prevents the neurotoxicity of {beta}-amyloid and glutamate receptor agonists: activation of GABA receptors and possible implications for Alzheimer's disease and other neurological disorders FASEB J, March 1, 2004; 18(3): 511 - 518. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help