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The Journal of Neuroscience, November 1, 1999, 19(21):9530-9537

Learning Induces a CDC2-Related Protein Kinase, KKIAMRE

Hiroshi Gomi^{1, 2}, William Sun^{1, 3}, Caleb E. Finch¹, Shigeyoshi Itohara², Kenji Yoshimi^{1, 4}, and Richard F. Thompson¹

¹ Neuroscience Program, University of Southern California, Los Angeles, California 90089-2520, ² Laboratory for Behavioral Genetics, RIKEN Brain Science Institute, Wako, Japan, ³ Metabasis Therapeutics, Inc., San Diego, California 92121, and ⁴ Neuroscience Research Laboratories, Sankyo Co., Ltd., Tokyo 140, Japan

To elucidate molecular mechanisms in learning and memory, we analyzed expression of mRNAs in brains of rabbits undergoing eyeblink conditioning. Infusion of the transcription inhibitor actinomycin D into the cerebellar interpositus nucleus reversibly blocked learning but not performance of the conditioned response. Differential display PCR analysis of cerebellar interpositus RNAs from trained and pseudotrained rabbits identified a 207 bp band that was induced with learning. The fragment was used to isolate a cDNA from a gt11 rabbit brain library containing a 1698 bp open reading frame. The deduced amino acid sequence contains the KKIAMRE motif, which is conserved among cell division cycle 2 (cdc2)-related kinases. These results suggest that there is a new category of cdc2-related kinases in the brain whose function may be important in learning and memory.

Key words: cerebellum; eyeblink conditioning; interpositus nucleus; RNA synthesis inhibition; learning-induced protein synthesis; KKIAMRE motif

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Copyright © 1999 Society for Neuroscience  0270-6474/99/19219530-08$05.00/0

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