QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (45) Citing Articles via Google Scholar Google Scholar Articles by Day, H. E. W. Articles by Akil, H. Search for Related Content PubMed PubMed Citation Articles by Day, H. E. W. Articles by Akil, H.

 Previous Article  |  Next Article 

The Journal of Neuroscience, November 15, 1999, 19(22):10098-10106

Expression of _1b Adrenoceptor mRNA in Corticotropin-Releasing Hormone-Containing Cells of the Rat Hypothalamus and Its Regulation by Corticosterone

Heidi E. W. Day, Serge Campeau, Stanley J. Watson Jr, and Huda Akil

Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan 48109-0720

Considerable evidence supports a role for brainstem adrenergic and noradrenergic inputs to corticotropin-releasing hormone (CRH) cells of the hypothalamic paraventricular nucleus (PVN), in the control of hypothalamic-pituitary-adrenocortical (HPA) axis function. However, little is known about specific adrenoceptor (ADR) subtypes in CRH-containing cells of the PVN. Here we demonstrate, using dual in situ hybridization, that mRNA encoding _1b ADR is colocalized with CRH in the rat PVN. Furthermore, we confirm that these _1b ADR mRNA-containing cells are stress-responsive, by colocalization with c-fos mRNA after restraint, swim, or immune stress.

To determine whether expression of _1b ADR mRNA is influenced by circulating glucocorticoids, male rats underwent bilateral adrenalectomy (ADX) or sham surgery, and were killed after 1, 3, 7, or 14 d. In situ hybridization revealed levels of _1b ADR mRNA were increased in the PVN 7 and 14 d after ADX, but were not altered in the hippocampus, amygdala, or dorsal raphe. Additional rats underwent ADX or sham surgery and received a corticosterone pellet (10 or 50 mg) or placebo for 7 d. Corticosterone replacement (10 mg) reduced the ADX-induced increase in PVN _1b ADR mRNA to control levels, whereas 50 mg of corticosterone replacement resulted in a decrease in PVN _1b ADR mRNA as compared with all other groups. Furthermore, levels of plasma corticosterone were significantly correlated (inverse relationship) with _1b ADR mRNA in the PVN.

We conclude that _1b ADR mRNA is expressed in CRH-containing, stress-responsive cells of the PVN and is highly sensitive to circulating levels of corticosterone. Because activation of the _1B adrenoceptor is predominantly excitatory within the brain, we predict that this receptor plays an important role in facilitation of the HPA axis response.

Key words: adrenergic; adrenoceptor; HPA axis; stress; CRH; hypothalamus; paraventricular nucleus; in situ hybridization; adrenalectomy; glucocorticoid; corticosterone

---

Copyright © 1999 Society for Neuroscience  0270-6474/99/192210098-09$05.00/0

This article has been cited by other articles:

				Y. Liu, A. Kamitakahara, A. J. Kim, and G. Aguilera Cyclic Adenosine 3',5'-Monophosphate Responsive Element Binding Protein Phosphorylation Is Required But Not Sufficient for Activation of Corticotropin-Releasing Hormone Transcription Endocrinology, July 1, 2008; 149(7): 3512 - 3520. [Abstract] [Full Text] [PDF]

				J. H. Yang, L. H. Li, S. Y. Shin, S. Lee, S. Y. Lee, S. K. Han, and P. D. Ryu Adrenalectomy Potentiates Noradrenergic Suppression of GABAergic Transmission in Parvocellular Neurosecretory Neurons of Hypothalamic Paraventricular Nucleus J Neurophysiol, February 1, 2008; 99(2): 514 - 523. [Abstract] [Full Text] [PDF]

				A. M. Khan, T. A. Ponzio, G. Sanchez-Watts, B. G. Stanley, G. I. Hatton, and A. G. Watts Catecholaminergic Control of Mitogen-Activated Protein Kinase Signaling in Paraventricular Neuroendocrine Neurons In Vivo and In Vitro: A Proposed Role during Glycemic Challenges J. Neurosci., July 4, 2007; 27(27): 7344 - 7360. [Abstract] [Full Text] [PDF]

				Q. Chen, D.-P. Li, and H.-L. Pan Presynaptic {alpha}1 Adrenergic Receptors Differentially Regulate Synaptic Glutamate and GABA Release to Hypothalamic Presympathetic Neurons J. Pharmacol. Exp. Ther., February 1, 2006; 316(2): 733 - 742. [Abstract] [Full Text] [PDF]

				G. Wittmann, Z. Liposits, R. M. Lechan, and C. Fekete Origin of Cocaine- and Amphetamine-Regulated Transcript-Containing Axons Innervating Hypophysiotropic Corticotropin-Releasing Hormone-Synthesizing Neurons in the Rat Endocrinology, July 1, 2005; 146(7): 2985 - 2991. [Abstract] [Full Text] [PDF]

				S. J. Judge, C. D. Ingram, and S. E. Gartside Moderate differences in circulating corticosterone alter receptor-mediated regulation of 5-hydroxytryptamine neuronal activity J Psychopharmacol, December 1, 2004; 18(4): 475 - 483. [Abstract] [PDF]

				A. M. Khan and A. G. Watts Intravenous 2-Deoxy-D-Glucose Injection Rapidly Elevates Levels of the Phosphorylated Forms of p44/42 Mitogen-Activated Protein Kinases (Extracellularly Regulated Kinases 1/2) in Rat Hypothalamic Parvicellular Paraventricular Neurons Endocrinology, January 1, 2004; 145(1): 351 - 359. [Abstract] [Full Text] [PDF]

				S. J. Evans, S. J. Watson, and H. Akil Evaluation of Sensitivity, Performance and Reproducibility of Microarray Technology in Neuronal Tissue Integr. Comp. Biol., December 1, 2003; 43(6): 780 - 785. [Abstract] [Full Text] [PDF]

				S. Ritter, A. G. Watts, T. T. Dinh, G. Sanchez-Watts, and C. Pedrow Immunotoxin Lesion of Hypothalamically Projecting Norepinephrine and Epinephrine Neurons Differentially Affects Circadian and Stressor-Stimulated Corticosterone Secretion Endocrinology, April 1, 2003; 144(4): 1357 - 1367. [Abstract] [Full Text] [PDF]

				R. L. Cole and P. E. Sawchenko Neurotransmitter Regulation of Cellular Activation and Neuropeptide Gene Expression in the Paraventricular Nucleus of the Hypothalamus J. Neurosci., February 1, 2002; 22(3): 959 - 969. [Abstract] [Full Text] [PDF]

				S. Avishai-Eliner, M. Eghbal-Ahmadi, E. Tabachnik, K. L. Brunson, and T. Z. Baram Down-Regulation of Hypothalamic Corticotropin-Releasing Hormone Messenger Ribonucleic Acid (mRNA) Precedes Early-Life Experience-Induced Changes in Hippocampal Glucocorticoid Receptor mRNA Endocrinology, January 1, 2001; 142(1): 89 - 97. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help