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The Journal of Neuroscience, December 1, 1999, 19(23):10318-10323

Cerebellar Granule Cell-Specific and Inducible Expression of Cre Recombinase in the Mouse

Mika Tsujita¹, Hisashi Mori¹, Masahiko Watanabe², Misao Suzuki³, Jun-ichi Miyazaki⁴, and Masayoshi Mishina^{1, 5}

¹ Department of Molecular Neurobiology and Pharmacology, School of Medicine, University of Tokyo, Tokyo 113-0033, Japan, ² Department of Anatomy, Hokkaido University, School of Medicine, Sapporo 060-8638, Japan, ³ Center for Animal Resources and Development, Kumamoto University, Kumamoto 860-0811, Japan, ⁴ Department of Nutrition and Physiological Chemistry, Osaka University Medical School, Osaka 565-0871, Japan, and ⁵ CREST, Japan Science and Technology Corporation, Saitama 332-0012, Japan

To develop a cell type-specific and temporal regulation system of gene targeting in the cerebellum, we used the NMDA-type glutamate receptor GluR3 subunit gene and Cre recombinase-progesterone receptor fusion (CrePR) gene in combination. Injection of the CrePR gene placed under the control of the 10 kb 5' region of the GluR3 gene into C57BL/6 eggs yielded the ECP25 line that strongly expressed the CrePR mRNA selectively in the granule cells of the cerebellum. Using a transgenic mouse carrying a reporter gene for Cre-mediated recombination, we showed that antiprogestins could induce the recombinase activity of CrePR protein in the cerebellar granule cells of the ECP25 line. Thus, the established mouse line will provide a valuable tool to investigate the mechanism of cerebellar function by manipulating molecules in the temporally regulated and granule cell-specific manner.

Key words: Cre recombinase; antiprogestin; cerebellum; cerebellar granule cell; gene targeting; NMDA receptor GluR3 subunit; progesterone receptor; transgenic mouse

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Copyright © 1999 Society for Neuroscience  0270-6474/99/192310318-06$05.00/0

This article has been cited by other articles:

				T. Takeuchi, T. Miyazaki, M. Watanabe, H. Mori, K. Sakimura, and M. Mishina Control of Synaptic Connection by Glutamate Receptor {delta}2 in the Adult Cerebellum J. Neurosci., February 23, 2005; 25(8): 2146 - 2156. [Abstract] [Full Text] [PDF]

				T. Minamino, V. Gaussin, F. J. DeMayo, and M. D. Schneider Inducible Gene Targeting in Postnatal Myocardium by Cardiac-Specific Expression of a Hormone-Activated Cre Fusion Protein Circ. Res., March 30, 2001; 88(6): 587 - 592. [Abstract] [Full Text] [PDF]

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