 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, December 1, 1999, 19(23):10438-10450
The Contribution of Facilitation of Monosynaptic PSPs to Dishabituation and Sensitization of the Aplysia Siphon Withdrawal Reflex
Igor Antonov1, Eric R. Kandel1, 2, 3, and Robert D. Hawkins1, 2 1 Center for Neurobiology and Behavior, College of Physicians and Surgeons, Columbia University, New York, New York 10032, and 2 New York State Psychiatric Institute and 3 Howard Hughes Medical Institute, New York, New York 10032
To examine the relationship between synaptic plasticity and learning and memory as directly as possible, we have developed a new simplified preparation for studying the siphon-withdrawal reflex of Aplysia in which it is relatively easy to record synaptic connections between individual identified neurons during simple forms of learning. We estimated that monosynaptic EPSPs from LE siphon sensory neurons to LFS siphon motor neurons mediate approximately one-third of the reflex response measured in this preparation, which corresponds to siphon flaring in the intact animal. To investigate cellular mechanisms contributing to dishabituation and sensitization, we recorded evoked firing of LFS neurons, the siphon withdrawal produced by stimulation of an LFS neuron, the complex PSP in an LFS neuron, and the monosynaptic PSP from an "on-field" or "off-field" LE neuron to an LFS neuron during behavioral training. Unlike the simplified gill-withdrawal preparation (Cohen et al., 1997; Frost et al., 1997), in the siphon-withdrawal preparation we found no qualitative differences between the major cellular mechanisms contributing to dishabituation and sensitization, suggesting that dissociations that have been observed previously may be attributable to transient inhibition that does not occur for this component of the reflex. Furthermore, in the siphon-withdrawal preparation, all of the various cellular measures, including monosynaptic PSPs from either on-field or off-field LE neurons, changed approximately in parallel with changes in the behavior. These results provide the most direct evidence so far available that both dishabituation and sensitization involve multiple mechanisms, including heterosynaptic facilitation of sensory neuron-motor neuron PSPs.
Key words: facilitation; PSP; dishabituation; sensitization; Aplysia; learning
Copyright © 1999 Society for Neuroscience 0270-6474/99/192310438-13$05.00/0
This article has been cited by other articles:
D. Fulton, M. C. Condro, K. Pearce, and D. L. Glanzman
The Potential Role of Postsynaptic Phospholipase C Activity in Synaptic Facilitation and Behavioral Sensitization in Aplysia
J Neurophysiol, July 1, 2008; 100(1): 108 - 116.
[Abstract] [Full Text] [PDF]
I. Antonov, T. Ha, I. Antonova, L. L. Moroz, and R. D. Hawkins
Role of Nitric Oxide in Classical Conditioning of Siphon Withdrawal in Aplysia
J. Neurosci., October 10, 2007; 27(41): 10993 - 11002.
[Abstract] [Full Text] [PDF]
E. G. Antzoulatos, M. L. Wainwright, L. J. Cleary, and J. H. Byrne
Long-term sensitization training primes Aplysia for further learning
Learn. Mem., July 1, 2006; 13(4): 422 - 425.
[Abstract] [Full Text] [PDF]
R. D. Hawkins, E. R. Kandel, and C. H. Bailey
Molecular Mechanisms of Memory Storage in Aplysia
Biol. Bull., June 1, 2006; 210(3): 174 - 191.
[Abstract] [Full Text] [PDF]
R. D. Hawkins, T. E. Cohen, and E. R. Kandel
Dishabituation in Aplysia can involve either reversal of habituation or superimposed sensitization
Learn. Mem., May 1, 2006; 13(3): 397 - 403.
[Abstract] [Full Text] [PDF]
R. D. Hawkins, G. A. Clark, and E. R. Kandel
Operant Conditioning of Gill Withdrawal in Aplysia
J. Neurosci., March 1, 2006; 26(9): 2443 - 2448.
[Abstract] [Full Text] [PDF]
G. A. Phares and J. H. Byrne
Analysis of 5-HT-Induced Short-Term Facilitation at Aplysia Sensorimotor Synapse During Bursts: Increased Synaptic Gain That Does Not Require ERK Activation
J Neurophysiol, July 1, 2005; 94(1): 871 - 877.
[Abstract] [Full Text] [PDF]
Q. Li, A. C. Roberts, and D. L. Glanzman
Synaptic Facilitation and Behavioral Dishabituation in Aplysia: Dependence on Release of Ca2+ from Postsynaptic Intracellular Stores, Postsynaptic Exocytosis, and Modulation of Postsynaptic AMPA Receptor Efficacy
J. Neurosci., June 8, 2005; 25(23): 5623 - 5637.
[Abstract] [Full Text] [PDF]
A. S. Bristol and T. J. Carew
Differential role of inhibition in habituation of two independent afferent pathways to a common motor output
Learn. Mem., January 1, 2005; 12(1): 52 - 60.
[Abstract] [Full Text] [PDF]
A. S. Bristol, M. A. Sutton, and T. J. Carew
Neural Circuit of Tail-Elicited Siphon Withdrawal in Aplysia. I. Differential Lateralization of Sensitization and Dishabituation
J Neurophysiol, February 1, 2004; 91(2): 666 - 677.
[Abstract] [Full Text] [PDF]
A. S. Bristol, S. Marinesco, and T. J. Carew
Neural Circuit of Tail-Elicited Siphon Withdrawal in Aplysia. II. Role of Gated Inhibition in Differential Lateralization of Sensitization and Dishabituation
J Neurophysiol, February 1, 2004; 91(2): 678 - 692.
[Abstract] [Full Text] [PDF]
Y. Ezzeddine and D. L. Glanzman
Prolonged Habituation of the Gill-Withdrawal Reflex in Aplysia Depends on Protein Synthesis, Protein Phosphatase Activity, and Postsynaptic Glutamate Receptors
J. Neurosci., October 22, 2003; 23(29): 9585 - 9594.
[Abstract] [Full Text] [PDF]
G. A. Phares, E. G. Antzoulatos, D. A. Baxter, and J. H. Byrne
Burst-Induced Synaptic Depression and Its Modulation Contribute to Information Transfer at Aplysia Sensorimotor Synapses: Empirical and Computational Analyses
J. Neurosci., September 10, 2003; 23(23): 8392 - 8401.
[Abstract] [Full Text] [PDF]
E. G. Antzoulatos, L. J. Cleary, A. Eskin, D. A. Baxter, and J. H. Byrne
Desensitization of Postsynaptic Glutamate Receptors Contributes to High-Frequency Homosynaptic Depression of Aplysia Sensorimotor Connections
Learn. Mem., September 1, 2003; 10(5): 309 - 313.
[Abstract] [Full Text] [PDF]
D. Barbas, L. DesGroseillers, V. F. Castellucci, T. J. Carew, and S. Marinesco
Multiple Serotonergic Mechanisms Contributing to Sensitization in Aplysia: Evidence of Diverse Serotonin Receptor Subtypes
Learn. Mem., September 1, 2003; 10(5): 373 - 386.
[Abstract] [Full Text] [PDF]
R. Nargeot
Voltage-Dependent Switching of Sensorimotor Integration by a Lobster Central Pattern Generator
J. Neurosci., June 15, 2003; 23(12): 4803 - 4808.
[Abstract] [Full Text] [PDF]
I. Antonov, I. Antonova, E. R. Kandel, and R. D. Hawkins
The Contribution of Activity-Dependent Synaptic Plasticity to Classical Conditioning in Aplysia
J. Neurosci., August 15, 2001; 21(16): 6413 - 6422.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|