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The Journal of Neuroscience, December 15, 1999, 19(24):10672-10679

Differences in Expression of Acetylcholinesterase and Collagen Q Control the Distribution and Oligomerization of the Collagen-Tailed Forms in Fast and Slow Muscles

Eric Krejci¹, Claire Legay¹, Sébastien Thomine¹, Janez Sketelj², and Jean Massoulié¹

¹ Laboratoire de Neurobiologie Cellulaire et Moléculaire, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8544, Ecole Normale Supérieure, 75005 Paris, France, and ² Institute of Pathophysiology, School of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia

The collagen-tailed forms of acetylcholinesterase (AChE) are accumulated at mammalian neuromuscular junctions. The A₄, A₈, and A₁₂ forms are expressed differently in the rat fast and slow muscles; the sternomastoid muscle contains essentially the A₁₂ form at end plates, whereas the soleus muscle also contains extrajunctional A₄ and A₈ forms. We show that collagen Q (ColQ) transcripts become exclusively junctional in the adult sternomastoid but remain uniformly expressed in the soleus. By coinjecting Xenopus oocytes with AChE_T and ColQ mRNAs, we reproduced the muscle patterns of collagen-tailed forms. The soleus contains transcripts ColQ1 and ColQ1a, whereas the sternomastoid only contains ColQ1a. Collagen-tailed AChE represents the first evidence that synaptic components involved in cholinergic transmission may be differently regulated in fast and slow muscles.

Key words: neuromuscular junction; acetylcholinesterase; collagen; fast muscle; slow muscle; rat

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Copyright © 1999 Society for Neuroscience  0270-6474/99/192410672-08$05.00/0

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