 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, December 15, 1999, 19(24):10829-10842
Synaptic Density in Geniculocortical Afferents Remains Constant after Monocular Deprivation in the Cat
Michael A. Silver and Michael P. Stryker W. M. Keck Center for Integrative Neuroscience and Neuroscience Graduate Program, Department of Physiology, University of California, San Francisco, California 94143-0444
Monocular eyelid closure in cats during a critical period in development produces both physiological plasticity, as indicated by a loss of responsiveness of primary visual cortical neurons to deprived eye stimulation, and morphological plasticity, as demonstrated by a decrease in the total length of individual geniculocortical arbors representing the deprived eye. Although the physiological plasticity appears maximal after 2 d of monocular deprivation (MD), the shrinkage of deprived-eye geniculocortical arbors is less than half-maximal at 4 d and is not maximal until 7 d of deprivation, at which time the deprived arbors are approximately half their previous size. To study this form of plasticity at the level of individual thalamocortical synapses rather than arbors, we developed a new double-label colocalization technique. First, geniculocortical afferent arbors serving either the deprived or nondeprived eye were labeled by injection of the anterograde tracer Phaseolus vulgaris leucoagglutinin into lamina A of the lateral geniculate nucleus. Then, using antibodies to synaptic vesicle proteins, we identified presynaptic terminals within the labeled arbors in layer IV of the primary visual cortex. Analysis of serial optical sections obtained using confocal microscopy allowed measurement of the numerical density of presynaptic sites and the relative amounts of synaptic vesicle protein in geniculocortical afferents after both 2 and 7 d of MD. We found that the density of synapses in geniculocortical axons was similar for deprived and nondeprived afferents, suggesting that this feature of the afferents is conserved even during periods in which synapse number is reduced by half in deprived-eye arbors. These results are not consistent with the hypothesis that a rapid loss of deprived-eye geniculocortical presynaptic sites is responsible for the prompt physiological effects of MD.
Key words: monocular deprivation; ocular dominance plasticity; presynaptic terminal; synaptic vesicle protein; cortical plasticity; primary visual cortex; lateral geniculate nucleus
Copyright © 1999 Society for Neuroscience 0270-6474/99/192410829-14$05.00/0
This article has been cited by other articles:
L. Cnops, T.-T. Hu, K. Burnat, and L. Arckens
Influence of Binocular Competition on the Expression Profiles of CRMP2, CRMP4, Dyn I, and Syt I in Developing Cat Visual Cortex
Cereb Cortex, May 1, 2008; 18(5): 1221 - 1231.
[Abstract] [Full Text] [PDF]
D. Dessem, M. Moritani, and R. Ambalavanar
Nociceptive Craniofacial Muscle Primary Afferent Neurons Synapse in Both the Rostral and Caudal Brain Stem
J Neurophysiol, July 1, 2007; 98(1): 214 - 223.
[Abstract] [Full Text] [PDF]
B. Zhang, H. Bi, E. Sakai, I. Maruko, J. Zheng, E. L. Smith III, and Y. M. Chino
Rapid plasticity of binocular connections in developing monkey visual cortex (V1)
PNAS, June 21, 2005; 102(25): 9026 - 9031.
[Abstract] [Full Text] [PDF]
M. T. Colonnese, J.-P. Zhao, and M. Constantine-Paton
NMDA Receptor Currents Suppress Synapse Formation on Sprouting Axons In Vivo
J. Neurosci., February 2, 2005; 25(5): 1291 - 1303.
[Abstract] [Full Text] [PDF]
A. H. Weiss and J. P. Kelly
Spatial-Frequency-Dependent Changes in Cortical Activation before and after Patching in Amblyopic Children
Invest. Ophthalmol. Vis. Sci., October 1, 2004; 45(10): 3531 - 3537.
[Abstract] [Full Text] [PDF]
B. T. Barrett, A. Bradley, and P. V. McGraw
Understanding the Neural Basis of Amblyopia
Neuroscientist, April 1, 2004; 10(2): 106 - 117.
[Abstract] [PDF]
S. Cohen-Cory
The Developing Synapse: Construction and Modulation of Synaptic Structures and Circuits
Science, October 25, 2002; 298(5594): 770 - 776.
[Abstract] [Full Text] [PDF]
M. Ohshima, Y. Hata, S. Ichisaka, M. Wakita, M. Fukuda, K. Kameyama, and T. Tsumoto
Chronic Electrical Stimulation of Afferents From One Eye Changes Ocular Dominance of Visual Cortical Neurons in Kittens
J Neurophysiol, October 1, 2002; 88(4): 2147 - 2151.
[Abstract] [Full Text] [PDF]
J. T. Trachtenberg and M. P. Stryker
Rapid Anatomical Plasticity of Horizontal Connections in the Developing Visual Cortex
J. Neurosci., May 15, 2001; 21(10): 3476 - 3482.
[Abstract] [Full Text] [PDF]
C. D. Hohnke, S. Oray, and M. Sur
Activity-Dependent Patterning of Retinogeniculate Axons Proceeds with a Constant Contribution from AMPA and NMDA Receptors
J. Neurosci., November 1, 2000; 20(21): 8051 - 8060.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|