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The Journal of Neuroscience, February 15, 1999, 19(4):1317-1323
Inhibitors of Myosin Light Chain Kinase Block Synaptic Vesicle Pool Mobilization during Action Potential Firing
Timothy A. Ryan Department of Biochemistry, The Weill Medical College of Cornell University, New York, New York 10021
During repetitive action potential firing the maintenance of synaptic transmission relies on a continued supply of synaptic vesicles for fusion with the presynaptic plasma membrane. The mechanism of transport by which vesicles are delivered to the site of fusion from a reserve pool is unknown, as are the biochemical pathways linking intracellular Ca2+ elevation with vesicle mobilization. Here, using the fluorescent tracer FM1-43 in hippocampal synaptic terminals, I show that inhibitors of myosin light chain kinase can block mobilization of the reserve pool and not the immediately releasable pool.
Key words: exocytosis; vesicle recycling; synaptic vesicles; myosin light chain kinase; hippocampus; presynaptic terminal; FM1-43
Copyright © 1999 Society for Neuroscience 0270-6474/99/1941317-07$05.00/0
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