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The Journal of Neuroscience, March 1, 1999, 19(5):1675-1690
Somatic Recording of GABAergic Autoreceptor Current in Cerebellar
Stellate and Basket Cells
Christophe
Pouzat and
Alain
Marty
Arbeitsgruppe Zelluläre Neurobiologie, Max-Planck-Institut
für Biophysikalische Chemie, 37077 Göttingen, Germany
Patch-clamp recordings were performed from stellate and basket
cells in rat cerebellar slices. Under somatic voltage clamp, short
depolarizing pulses were applied to elicit action potentials in the
axon. After the action potential, a bicuculline- and
Cd2+-sensitive current transient was observed. A
similar response was obtained when eliciting axonal firing by
extracellular stimulation. With an isotonic internal
Cl solution, the peak amplitude of this current
varied linearly with the holding potential, yielding an extrapolated
reversal potential of  20 to 0 mV. Unlike synaptic or autaptic
GABAergic currents obtained in the same preparation, the current
transient had a slow rise-time and a low variability between trials.
This current was blocked when 10 mM BAPTA was included in
the recording solution. In some experiments, the current transient
elicited axonal action potentials. The current transient was reliably
observed in animals aged 12-15 d, with a mean amplitude of 82 pA at
70 mV, but was small and rare in the age group 29-49 d. Numerical simulations could account for all properties of the current transient by assuming that an action potential activates a distributed GABAergic conductance in the axon. The actual conductance is probably restricted to release sites, with an estimated mean presynaptic current response of 10 pA per site (70 mV, age 12-15 d). We conclude that in
developing rats, stellate and basket cell axons have a high density of
GABAergic autoreceptors and that a sizable fraction of the
corresponding current can be measured from the soma.
Key words:
cerebellum; basket cell; stellate cell; GABA; IPSCs; axon; autoreceptors; autapses
Copyright © 1999 Society for Neuroscience 0270-6474/99/1951675-16$05.00/0
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