 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
The Journal of Neuroscience, March 1, 1999, 19(5):1844-1854
Differential Expression of the mRNA for the Vanilloid Receptor Subtype 1 in Cells of the Adult Rat Dorsal Root and Nodose Ganglia and Its Downregulation by Axotomy
Gregory J. Michael and John V. Priestley Neuroscience Section, Division of Biomedical Sciences, Queen Mary and Westfield College, London E1 4NS, United Kingdom
Sensitivity to the pungent vanilloid, capsaicin, defines a subpopulation of primary sensory neurons that are mainly polymodal nociceptors. The recently cloned vanilloid receptor subtype 1 (VR1) is activated by capsaicin and noxious heat. Using combined in situ hybridization and histochemical methods, we have characterized in sensory ganglia the expression of VR1 mRNA. We show that this receptor is almost exclusively expressed by neurofilament-negative small- and medium-sized dorsal root ganglion cells. Within this population, VR1 mRNA is detected at widely varying levels in both the NGF receptor (trkA)-positive, peptide-producing cells that elicit neurogenic inflammation and the functionally less characterized glial cell line-derived neurotrophic factor-responsive cells that bind lectin Griffonia simplicifolia isolectin B4 (IB4). Cells without detectable levels of VR1 mRNA are found in both classes. A subpopulation of the IB4-binding cells that produce somatostatin has relatively low levels of VR1 mRNA. A previously uncharacterized population of very small cells that express the receptor tyrosine kinase (RET) and that do not label for trkA or IB4-binding has the highest relative levels of VR1 mRNA. The majority of small visceral sensory neurons of the nodose ganglion also express VR1 mRNA, in conjunction with the BDNF receptor trkB but not trkA. Axotomy results in the downregulation of VR1 mRNA in dorsal root ganglion cells.
Our data emphasize the heterogeneity of VR1 mRNA expression by subclasses of small sensory neurons, and this may result in their differential sensitivity to chemical and noxious heat stimuli. Our results also indicate that peripherally derived trophic factors may regulate levels of VR1 mRNA.
Key words: axotomy; capsaicin; immunocytochemistry; in situ hybridization; nociception; sensory neuron subpopulations; vanilloid receptor; VR1
Copyright © 1999 Society for Neuroscience 0270-6474/99/1951844-11$05.00/0
This article has been cited by other articles:
I. J. Lever, M. Robinson, M. Cibelli, C. Paule, P. Santha, L. Yee, S. P. Hunt, B. F. Cravatt, M. R. Elphick, I. Nagy, et al.
Localization of the Endocannabinoid-Degrading Enzyme Fatty Acid Amide Hydrolase in Rat Dorsal Root Ganglion Cells and Its Regulation after Peripheral Nerve Injury
J. Neurosci., March 25, 2009; 29(12): 3766 - 3780.
[Abstract] [Full Text] [PDF]
G. Zhang, R.-L. Lin, M. Wiggers, D. M. Snow, and L.-Y. Lee
Altered expression of TRPV1 and sensitivity to capsaicin in pulmonary myelinated afferents following chronic airway inflammation in the rat
J. Physiol., December 1, 2008; 586(23): 5771 - 5786.
[Abstract] [Full Text] [PDF]
M. Larsson and J. Broman
Translocation of GluR1-Containing AMPA Receptors to a Spinal Nociceptive Synapse during Acute Noxious Stimulation
J. Neurosci., July 9, 2008; 28(28): 7084 - 7090.
[Abstract] [Full Text] [PDF]
M. A. Williams, S. A. Smith, D. E. O'Brien, J. H. Mitchell, and M. G. Garry
The group IV afferent neuron expresses multiple receptor alterations in cardiomyopathyic rats: evidence at the cannabinoid CB1 receptor
J. Physiol., February 1, 2008; 586(3): 835 - 845.
[Abstract] [Full Text] [PDF]
T. Sugiura, K. Bielefeldt, and G. F. Gebhart
Mouse colon sensory neurons detect extracellular acidosis via TRPV1
Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1768 - C1774.
[Abstract] [Full Text] [PDF]
K. K. Rau, N. Jiang, R. D. Johnson, and B. Y. Cooper
Heat Sensitization in Skin and Muscle Nociceptors Expressing Distinct Combinations of TRPV1 and TRPV2 Protein
J Neurophysiol, April 1, 2007; 97(4): 2651 - 2662.
[Abstract] [Full Text] [PDF]
S. A. Malin, D. C. Molliver, H. R. Koerber, P. Cornuet, R. Frye, K. M. Albers, and B. M. Davis
Glial Cell Line-Derived Neurotrophic Factor Family Members Sensitize Nociceptors In Vitro and Produce Thermal Hyperalgesia In Vivo.
J. Neurosci., August 15, 2006; 26(33): 8588 - 8599.
[Abstract] [Full Text] [PDF]
M. Covasa
CCK- and leptin-induced vagal afferent activation: a model for organ-specific endocrine modulation of visceral sensory information
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2006; 290(6): R1542 - R1543.
[Full Text] [PDF]
M. Larsson and J. Broman
Pathway-specific bidirectional regulation of Ca2+/calmodulin-dependent protein kinase II at spinal nociceptive synapses after acute noxious stimulation.
J. Neurosci., April 19, 2006; 26(16): 4198 - 4205.
[Abstract] [Full Text] [PDF]
L. Gendron, A. L. Lucido, F. Mennicken, D. O'Donnell, J.-P. Vincent, T. Stroh, and A. Beaudet
Morphine and Pain-Related Stimuli Enhance Cell Surface Availability of Somatic {delta}-Opioid Receptors in Rat Dorsal Root Ganglia
J. Neurosci., January 18, 2006; 26(3): 953 - 962.
[Abstract] [Full Text] [PDF]
M. Pertin, R.-R. Ji, T. Berta, A. J. Powell, L. Karchewski, S. N. Tate, L. L. Isom, C. J. Woolf, N. Gilliard, D. R. Spahn, et al.
Upregulation of the Voltage-Gated Sodium Channel {beta}2 Subunit in Neuropathic Pain Models: Characterization of Expression in Injured and Non-Injured Primary Sensory Neurons
J. Neurosci., November 23, 2005; 25(47): 10970 - 10980.
[Abstract] [Full Text] [PDF]
Y. Zhu, Y. Wang, and D. H. Wang
Diuresis and Natriuresis Caused by Activation of VR1-Positive Sensory Nerves in Renal Pelvis of Rats
Hypertension, October 1, 2005; 46(4): 992 - 997.
[Abstract] [Full Text] [PDF]
P. Honore, C. T. Wismer, J. Mikusa, C. Z. Zhu, C. Zhong, D. M. Gauvin, A. Gomtsyan, R. El Kouhen, C.-H. Lee, K. Marsh, et al.
A-425619 [1-Isoquinolin-5-yl-3-(4-trifluoromethyl-benzyl)-urea], a Novel Transient Receptor Potential Type V1 Receptor Antagonist, Relieves Pathophysiological Pain Associated with Inflammation and Tissue Injury in Rats
J. Pharmacol. Exp. Ther., July 1, 2005; 314(1): 410 - 421.
[Abstract] [Full Text] [PDF]
Z.-Z. Wu, S.-R. Chen, and H.-L. Pan
Transient Receptor Potential Vanilloid Type 1 Activation Down-regulates Voltage-gated Calcium Channels through Calcium-dependent Calcineurin in Sensory Neurons
J. Biol. Chem., May 6, 2005; 280(18): 18142 - 18151.
[Abstract] [Full Text] [PDF]
K. Kwong and L.-Y. Lee
Prostaglandin E2 potentiates a TTX-resistant sodium current in rat capsaicin-sensitive vagal pulmonary sensory neurones
J. Physiol., April 15, 2005; 564(2): 437 - 450.
[Abstract] [Full Text] [PDF]
J. R. Ghilardi, H. Rohrich, T. H. Lindsay, M. A. Sevcik, M. J. Schwei, K. Kubota, K. G. Halvorson, J. Poblete, S. R. Chaplan, A. E. Dubin, et al.
Selective Blockade of the Capsaicin Receptor TRPV1 Attenuates Bone Cancer Pain
J. Neurosci., March 23, 2005; 25(12): 3126 - 3131.
[Abstract] [Full Text] [PDF]
T. Sugiura, K. Dang, K. Lamb, K. Bielefeldt, and G. F. Gebhart
Acid-Sensing Properties in Rat Gastric Sensory Neurons from Normal and Ulcerated Stomach
J. Neurosci., March 9, 2005; 25(10): 2617 - 2627.
[Abstract] [Full Text] [PDF]
T. D. Wilson-Gerwing, M. V. Dmyterko, D. W. Zochodne, J. M. Johnston, and V. M. K. Verge
Neurotrophin-3 Suppresses Thermal Hyperalgesia Associated with Neuropathic Pain and Attenuates Transient Receptor Potential Vanilloid Receptor-1 Expression in Adult Sensory Neurons
J. Neurosci., January 19, 2005; 25(3): 758 - 767.
[Abstract] [Full Text] [PDF]
D. A. Groneberg, A. Niimi, Q. T. Dinh, B. Cosio, M. Hew, A. Fischer, and K. F. Chung
Increased Expression of Transient Receptor Potential Vanilloid-1 in Airway Nerves of Chronic Cough
Am. J. Respir. Crit. Care Med., December 15, 2004; 170(12): 1276 - 1280.
[Abstract] [Full Text] [PDF]
R. S. Scotland, S. Chauhan, C. Davis, C. De Felipe, S. Hunt, J. Kabir, P. Kotsonis, U. Oh, and A. Ahluwalia
Vanilloid Receptor TRPV1, Sensory C-Fibers, and Vascular Autoregulation: A Novel Mechanism Involved in Myogenic Constriction
Circ. Res., November 12, 2004; 95(10): 1027 - 1034.
[Abstract] [Full Text] [PDF]
K. Obata, H. Yamanaka, K. Kobayashi, Y. Dai, T. Mizushima, H. Katsura, T. Fukuoka, A. Tokunaga, and K. Noguchi
Role of Mitogen-Activated Protein Kinase Activation in Injured and Intact Primary Afferent Neurons for Mechanical and Heat Hypersensitivity after Spinal Nerve Ligation
J. Neurosci., November 10, 2004; 24(45): 10211 - 10222.
[Abstract] [Full Text] [PDF]
T. Sugiuar, K. Bielefeldt, and G. F. Gebhart
TRPV1 Function in Mouse Colon Sensory Neurons Is Enhanced by Metabotropic 5-Hydroxytryptamine Receptor Activation
J. Neurosci., October 27, 2004; 24(43): 9521 - 9530.
[Abstract] [Full Text] [PDF]
V. Vellani, O. Zachrisson, and P. A McNaughton
Functional bradykinin B1 receptors are expressed in nociceptive neurones and are upregulated by the neurotrophin GDNF
J. Physiol., October 15, 2004; 560(2): 391 - 401.
[Abstract] [Full Text] [PDF]
J. Wang, Y. Ren, X. Zou, L. Fang, W. D. Willis, and Q. Lin
Sympathetic Influence on Capsaicin-Evoked Enhancement of Dorsal Root Reflexes in Rats
J Neurophysiol, October 1, 2004; 92(4): 2017 - 2026.
[Abstract] [Full Text] [PDF]
B Schmidt, J Hammer, P Holzer, and H F Hammer
Chemical nociception in the jejunum induced by capsaicin
Gut, August 1, 2004; 53(8): 1109 - 1116.
[Abstract] [Full Text] [PDF]
C. J. Woodbury, M. Zwick, S. Wang, J. J. Lawson, M. J. Caterina, M. Koltzenburg, K. M. Albers, H. R. Koerber, and B. M. Davis
Nociceptors Lacking TRPV1 and TRPV2 Have Normal Heat Responses
J. Neurosci., July 14, 2004; 24(28): 6410 - 6415.
[Abstract] [Full Text] [PDF]
L. Zhang, S. Jones, K. Brody, M. Costa, and S. J. H. Brookes
Thermosensitive transient receptor potential channels in vagal afferent neurons of the mouse
Am J Physiol Gastrointest Liver Physiol, June 1, 2004; 286(6): G983 - G991.
[Abstract] [Full Text] [PDF]
Y.-H. Jin, T. W. Bailey, B.-y. Li, J. H. Schild, and M. C. Andresen
Purinergic and Vanilloid Receptor Activation Releases Glutamate from Separate Cranial Afferent Terminals in Nucleus Tractus Solitarius
J. Neurosci., May 19, 2004; 24(20): 4709 - 4717.
[Abstract] [Full Text] [PDF]
Y.-M. Kang, K. Bielefeldt, and G. F. Gebhart
Sensitization of Mechanosensitive Gastric Vagal Afferent Fibers in the Rat by Thermal and Chemical Stimuli and Gastric Ulcers
J Neurophysiol, May 1, 2004; 91(5): 1981 - 1989.
[Abstract] [Full Text] [PDF]
G. P. Ahern
Activation of TRPV1 by the Satiety Factor Oleoylethanolamide
J. Biol. Chem., August 15, 2003; 278(33): 30429 - 30434.
[Abstract] [Full Text] [PDF]
J. R. Meyers, R. B. MacDonald, A. Duggan, D. Lenzi, D. G. Standaert, J. T. Corwin, and D. P. Corey
Lighting up the Senses: FM1-43 Loading of Sensory Cells through Nonselective Ion Channels
J. Neurosci., May 15, 2003; 23(10): 4054 - 4065.
[Abstract] [Full Text] [PDF]
K. Obata, H. Yamanaka, Y. Dai, T. Tachibana, T. Fukuoka, A. Tokunaga, H. Yoshikawa, and K. Noguchi
Differential Activation of Extracellular Signal-Regulated Protein Kinase in Primary Afferent Neurons Regulates Brain-Derived Neurotrophic Factor Expression after Peripheral Inflammation and Nerve Injury
J. Neurosci., May 15, 2003; 23(10): 4117 - 4126.
[Abstract] [Full Text] [PDF]
C. Labrakakis, C.-K. Tong, T. Weissman, C. Torsney, and A. B MacDermott
Localization and function of ATP and GABAA receptors expressed by nociceptors and other postnatal sensory neurons in rat
J. Physiol., May 15, 2003; 549(1): 131 - 142.
[Abstract] [Full Text] [PDF]
J. B. Morris, P. T. Symanowicz, J. E. Olsen, R. S. Thrall, M. M. Cloutier, and A. K. Hubbard
Immediate sensory nerve-mediated respiratory responses to irritants in healthy and allergic airway-diseased mice
J Appl Physiol, April 1, 2003; 94(4): 1563 - 1571.
[Abstract] [Full Text] [PDF]
S. Kerzel, G. Path, W. A. Nockher, D. Quarcoo, U. Raap, D. A. Groneberg, Q. T. Dinh, A. Fischer, A. Braun, and H. Renz
Pan-Neurotrophin Receptor p75 Contributes to Neuronal Hyperreactivity and Airway Inflammation in a Murine Model of Experimental Asthma
Am. J. Respir. Cell Mol. Biol., February 1, 2003; 28(2): 170 - 178.
[Abstract] [Full Text] [PDF]
S. Dirajlal, L. E. Pauers, and C. L. Stucky
Differential Response Properties of IB4-Positive and -Negative Unmyelinated Sensory Neurons to Protons and Capsaicin
J Neurophysiol, January 1, 2003; 89(1): 513 - 524.
[Abstract] [Full Text] [PDF]
L M Mendell
Does NGF binding to p75 and trkA receptors activate independent signalling pathways to sensitize nociceptors?
J. Physiol., October 15, 2002; 544(2): 333 - 333.
[Full Text] [PDF]
L. R. Watkins and S. F. Maier
Beyond Neurons: Evidence That Immune and Glial Cells Contribute to Pathological Pain States
Physiol Rev, October 1, 2002; 82(4): 981 - 1011.
[Abstract] [Full Text] [PDF]
M. Zwick, B. M. Davis, C. J. Woodbury, J. N. Burkett, H. R. Koerber, J. F. Simpson, and K. M. Albers
Glial Cell Line-Derived Neurotrophic Factor is a Survival Factor for Isolectin B4-Positive, but not Vanilloid Receptor 1-Positive, Neurons in the Mouse
J. Neurosci., May 15, 2002; 22(10): 4057 - 4065.
[Abstract] [Full Text] [PDF]
M. Pare, R. Elde, J. E. Mazurkiewicz, A. M. Smith, and F. L. Rice
The Meissner Corpuscle Revised: A Multiafferented Mechanoreceptor with Nociceptor Immunochemical Properties
J. Neurosci., September 15, 2001; 21(18): 7236 - 7246.
[Abstract] [Full Text] [PDF]
W. Magerl, P. N. Fuchs, R. A. Meyer, and R.-D. Treede
Roles of capsaicin-insensitive nociceptors in cutaneous pain and secondary hyperalgesia
Brain, September 1, 2001; 124(9): 1754 - 1764.
[Abstract] [Full Text] [PDF]
V Vlachova, A Lyfenko, R. Orkand, and L Vyklicky
The effects of capsaicin and acidity on currents generated by noxious heat in cultured neonatal rat dorsal root ganglion neurones
J. Physiol., June 15, 2001; 533(3): 717 - 728.
[Abstract] [Full Text] [PDF]
A. J. Kirkup, A. M. Brunsden, and D. Grundy
Receptors and Transmission in the Brain-Gut Axis: Potential for Novel Therapies: I. Receptors on visceral afferents
Am J Physiol Gastrointest Liver Physiol, May 1, 2001; 280(5): G787 - G794.
[Abstract] [Full Text] [PDF]
T. Kiso, Y. Nagakura, T. Toya, N. Matsumoto, S. Tamura, H. Ito, M. Okada, and T. Yamaguchi
Neurometer Measurement of Current Stimulus Threshold in Rats
J. Pharmacol. Exp. Ther., April 1, 2001; 297(1): 352 - 356.
[Abstract] [Full Text]
D. L. H. Bennett
Neurotrophic Factors: Important Regulators of Nociceptive Function
Neuroscientist, February 1, 2001; 7(1): 13 - 17.
[Abstract] [PDF]
M. J Gunthorpe, M. H Harries, R. K Prinjha, J. B Davis, and A. Randall
Voltage- and time-dependent properties of the recombinant rat vanilloid receptor (rVR1)
J. Physiol., June 15, 2000; 525(3): 747 - 759.
[Abstract] [Full Text] [PDF]
C. J. Woolf and M. W. Salter
Neuronal Plasticity: Increasing the Gain in Pain
Science, June 9, 2000; 288(5472): 1765 - 1768.
[Abstract] [Full Text] [PDF]
I. Nagy and H. P. Rang
Similarities and Differences between the Responses of Rat Sensory Neurons to Noxious Heat and Capsaicin
J. Neurosci., December 15, 1999; 19(24): 10647 - 10655.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|