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The Journal of Neuroscience, March 15, 1999, 19(6):2195-2208

Transient Potassium Currents Regulate the Discharge Patterns of Dorsal Cochlear Nucleus Pyramidal Cells

Patrick O. Kanold^{1, 2} and Paul B. Manis^{1, 2, 3}

¹ The Center for Hearing Sciences and Departments of ² Biomedical Engineering and ³ Otolaryngology-Head and Neck Surgery, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

Pyramidal cells in the dorsal cochlear nucleus (DCN) show three distinct temporal discharge patterns in response to sound: "pauser," "buildup," and "chopper." Similar discharge patterns are seen in vitro and depend on the voltage from which the cell is depolarized. It has been proposed that an inactivating A-type K⁺ current (I_KI) might play a critical role in generating the three different patterns. In this study we examined the characteristics of transient currents in DCN pyramidal cells to evaluate this hypothesis. Morphologically identified pyramidal cells in rat brain slices (P11-P17) exhibited the three voltage-dependent discharge patterns. Two inactivating currents were present in outside-out patches from pyramidal cells: a rapidly inactivating (I_KIF,  ~11 msec) current insensitive to block by tetraethylammonium (TEA) and variably blocked by 4-aminopyridine (4-AP) with half-inactivation near 85 mV, and a slowly inactivating TEA- and 4-AP-sensitive current (I_KIS,  ~145 msec) with half-inactivation near 35 mV. Recovery from inactivation at 34°C was described by a single exponential with a time constant of 10-30 msec, similar to the rate at which first spike latency increases with the duration of a hyperpolarizing prepulse. Acutely isolated cells also possessed a rapidly activating (<1 msec at 22°C) transient current that activated near 45 mV and showed half-inactivation near 80 mV. A model demonstrated that the deinactivation of I_KIF was correlated with the discharge patterns. Overall, the properties of the fast inactivating K⁺ current were consistent with their proposed role in shaping the discharge pattern of DCN pyramidal cells.

Key words: hearing; auditory system; cochlear nucleus; transient potassium currents; potassium channels; voltage-clamp; intrinsic discharge patterns; inactivation; outside-out patches

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Copyright © 1999 Society for Neuroscience  0270-6474/99/1962195-14$05.00/0

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