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The Journal of Neuroscience, April 1, 1999, 19(7):2658-2664
Stimulus-Dependent Translocation of
Opioid Receptors to the Plasma Membrane
Samuel J. Shuster1, 2, Maureen Riedl2, Xinren Li2, Lucy Vulchanova2, and Robert Elde1, 2 1 Graduate Program in Neuroscience and 2 Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis, Minnesota 55455
We examined the cellular and subcellular distribution of the cloned
opioid receptor (KOR1) and its trafficking to the presynaptic plasma membrane in vasopressin magnocellular neurosecretory neurons. We used immunohistochemistry to show that KOR1 immunoreactivity (IR) colocalized with vasopressin-containing cell bodies, axons, and axon terminals within the posterior pituitary. Ultrastructural analysis revealed that a major fraction of KOR1-IR was associated with the membrane of peptide-containing large secretory vesicles. KOR1-IR was rarely associated with the plasma membrane in unstimulated nerve terminals within the posterior pituitary. A physiological stimulus (salt-loading) that elicits vasopressin release also caused KOR1-IR to translocate from these vesicles to the plasma membrane. After stimulation, there was a significant decrease in KOR1-IR associated with peptide-containing vesicles and a significant increase in KOR1-IR associated with the plasma membrane. This stimulus-dependent translocation of receptors to the presynaptic plasma membrane provides a novel mechanism for regulation of transmitter release.
Key words: translocation;
Copyright © 1999 Society for Neuroscience 0270-6474/99/1972658-07$05.00/0
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