WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (75)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, Y.
Right arrow Articles by Lipton, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, Y.
Right arrow Articles by Lipton, P.

 Previous Article  |  Next Article 

The Journal of Neuroscience, May 1, 1999, 19(9):3307-3315

Cytosolic Ca2+ Changes during In Vitro Ischemia in Rat Hippocampal Slices: Major Roles for Glutamate and Na+-Dependent Ca2+ Release from Mitochondria

Yanlong Zhang and Peter Lipton

Department of Physiology, University of Wisconsin Medical School, Madison, Wisconsin 53706

This work determined Ca2+ transport processes that contribute to the rise in cytosolic Ca2+ during in vitro ischemia (deprivation of oxygen and glucose) in the hippocampus. The CA1 striatum radiatum of rat hippocampal slices was monitored by confocal microscopy of calcium green-1. There was a 50-60% increase in fluorescence during 10 min of ischemia after a 3 min lag period. During the first 5 min of ischemia the major contribution was from Ca2+ entering via NMDA receptors; most of the fluorescence increase was blocked by MK-801. Approximately one-half of the sustained increase in fluorescence during 10 min of ischemia was caused by activation of Ca2+ release from mitochondria via the mitochondrial 2Na+-Ca2+ exchanger. Inhibition of Na+ influx across the plasmalemma using lidocaine, low extracellular Na+, or the AMPA/kainate receptor blocker CNQX reduced the fluorescence increase by 50%. The 2Na+-Ca2+ exchange blocker CGP37157 also blocked the increase, and this effect was not additive with the effects of blocking Na+ influx. When added together, CNQX and lidocaine inhibited the fluorescence increase more than CGP37157 did. Thus, during ischemia, Ca2+ entry via NMDA receptors accounts for the earliest rise in cytosolic Ca2+. Approximately 50% of the sustained rise is attributable to Na+ entry and subsequent Ca2+ release from the mitochondria via the 2Na+-Ca2+ exchanger. Sodium entry is also hypothesized to compromise clearance of cytosolic Ca2+ by routes other than mitochondrial uptake, probably by enhancing ATP depletion, accounting for the large inhibition of the Ca2+ increase by the combination of CNQX and lidocaine.

Key words: NMDA; AMPA/kainate; mitochondria; CGP37157; sodium channels; CNQX

Copyright © 1999 Society for Neuroscience  0270-6474/99/1993307-09$05.00/0


This article has been cited by other articles:


Home page
 Cereb CortexHome page
D. Chao, A. Bazzy-Asaad, G. Balboni, S. Salvadori, and Y. Xia
Activation of DOR Attenuates Anoxic K+ Derangement via Inhibition of Na+ Entry in Mouse Cortex
Cereb Cortex, September 1, 2008; 18(9): 2217 - 2227.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
T. A. Vander Jagt, J. A. Connor, and C. W. Shuttleworth
Localized Loss of Ca2+ Homeostasis in Neuronal Dendrites Is a Downstream Consequence of Metabolic Compromise during Extended NMDA Exposures
J. Neurosci., May 7, 2008; 28(19): 5029 - 5039.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
I. Fishbein and M. Segal
Miniature Synaptic Currents Become Neurotoxic to Chronically Silenced Neurons
Cereb Cortex, June 1, 2007; 17(6): 1292 - 1306.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. G. Jackson and S. A. Thayer
Mitochondrial Modulation of Ca2+-Induced Ca2+-Release in Rat Sensory Neurons
J Neurophysiol, September 1, 2006; 96(3): 1093 - 1104.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. Misonou, D. P. Mohapatra, M. Menegola, and J. S. Trimmer
Calcium- and Metabolic State-Dependent Modulation of the Voltage-Dependent Kv2.1 Channel Regulates Neuronal Excitability in Response to Ischemia
J. Neurosci., November 30, 2005; 25(48): 11184 - 11193.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. A. Nikolaeva, B. Mukherjee, and P. K. Stys
Na+-Dependent Sources of Intra-Axonal Ca2+ Release in Rat Optic Nerve during In Vitro Chemical Ischemia
J. Neurosci., October 26, 2005; 25(43): 9960 - 9967.
[Abstract] [Full Text] [PDF]

Home page
 BrainHome page
M. Hamann, D. J. Rossi, C. Mohr, A. L. Andrade, and D. Attwell
The electrical response of cerebellar Purkinje neurons to simulated ischaemia
Brain, October 1, 2005; 128(10): 2408 - 2420.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
H. Jourdi, X. Lu, T. Yanagihara, J. C. Lauterborn, X. Bi, C. M. Gall, and M. Baudry
Prolonged Positive Modulation of {alpha}-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptors Induces Calpain-Mediated PSD-95/Dlg/ZO-1 Protein Degradation and AMPA Receptor Down-Regulation in Cultured Hippocampal Slices
J. Pharmacol. Exp. Ther., July 1, 2005; 314(1): 16 - 26.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
G. E. Hermann, J. S. Nasse, and R. C. Rogers
{alpha}-1 adrenergic input to solitary nucleus neurones: calcium oscillations, excitation and gastric reflex control
J. Physiol., January 15, 2005; 562(2): 553 - 568.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. Sheldon, A. Diarra, Y. M. Cheng, and J. Church
Sodium Influx Pathways during and after Anoxia in Rat Hippocampal Neurons
J. Neurosci., December 8, 2004; 24(49): 11057 - 11069.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
F. Galeffi, R. Sah, B. B. Pond, A. George, and R. D. Schwartz-Bloom
Changes in Intracellular Chloride after Oxygen-Glucose Deprivation of the Adult Hippocampal Slice: Effect of Diazepam
J. Neurosci., May 5, 2004; 24(18): 4478 - 4488.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Schild, J. Huppelsberg, S. Kahlert, G. Keilhoff, and G. Reiser
Brain Mitochondria Are Primed by Moderate Ca2+ Rise upon Hypoxia/Reoxygenation for Functional Breakdown and Morphological Disintegration
J. Biol. Chem., July 3, 2003; 278(28): 25454 - 25460.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
G.-F. Tian and A. J. Baker
Protective Effect of High Glucose Against Ischemia-Induced Synaptic Transmission Damage in Rat Hippocampal Slices
J Neurophysiol, July 1, 2002; 88(1): 236 - 248.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
C. Sheldon and J. Church
Intracellular pH Response to Anoxia in Acutely Dissociated Adult Rat Hippocampal CA1 Neurons
J Neurophysiol, May 1, 2002; 87(5): 2209 - 2224.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
W. Shen and M. M. Slaughter
A Non-Excitatory Paradigm of Glutamate Toxicity
J Neurophysiol, March 1, 2002; 87(3): 1629 - 1634.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
S. Qi, R.-Z. Zhan, C. Wu, H. Fujihara, K. Taga, and K. Shimoji
The Effects of Thiopental and Propofol on Cell Swelling Induced by Oxygen/Glucose Deprivation in the CA1 Pyramidal Cell Layer of Rat Hippocampal Slices
Anesth. Analg., March 1, 2002; 94(3): 655 - 660.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
G. J. Wang and S. A. Thayer
NMDA-Induced Calcium Loads Recycle Across the Mitochondrial Inner Membrane of Hippocampal Neurons in Culture
J Neurophysiol, February 1, 2002; 87(2): 740 - 749.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
K. M. Raley-Susman, I. S. Kass, J. E. Cottrell, R. B. Newman, G. Chambers, and J. Wang
Sodium Influx Blockade and Hypoxic Damage to CA1 Pyramidal Neurons in Rat Hippocampal Slices
J Neurophysiol, December 1, 2001; 86(6): 2715 - 2726.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. G. Somjen
Mechanisms of Spreading Depression and Hypoxic Spreading Depression-Like Depolarization
Physiol Rev, July 1, 2001; 81(3): 1065 - 1096.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
L. Tretter and V. Adam-Vizi
Inhibition of Krebs Cycle Enzymes by Hydrogen Peroxide: A Key Role of {alpha}-Ketoglutarate Dehydrogenase in Limiting NADH Production under Oxidative Stress
J. Neurosci., December 15, 2000; 20(24): 8972 - 8979.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
D. Tian, V. Litvak, and S. Lev
Cerebral Ischemia and Seizures Induce Tyrosine Phosphorylation of PYK2 in Neurons and Microglial Cells
J. Neurosci., September 1, 2000; 20(17): 6478 - 6487.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
S. Bahar, D. Fayuk, G. G. Somjen, P. G. Aitken, and D. A. Turner
Mitochondrial and Intrinsic Optical Signals Imaged During Hypoxia and Spreading Depression in Rat Hippocampal Slices
J Neurophysiol, July 1, 2000; 84(1): 311 - 324.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. Wang, G. Chambers, J. E. Cottrell, and I. S. Kass
Differential Fall in ATP Accounts for Effects of Temperature on Hypoxic Damage in Rat Hippocampal Slices
J Neurophysiol, June 1, 2000; 83(6): 3462 - 3472.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. Chinopoulos, L. Tretter, A. Rozsa, and V. Adam-Vizi
Exacerbated Responses to Oxidative Stress by an Na+ Load in Isolated Nerve Terminals: the Role of ATP Depletion and Rise of [Ca2+]i
J. Neurosci., March 15, 2000; 20(6): 2094 - 2103.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
P. Lipton
Ischemic Cell Death in Brain Neurons
Physiol Rev, October 1, 1999; 79(4): 1431 - 1568.
[Abstract] [Full Text] [PDF]


-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-