QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (122) Citing Articles via Google Scholar Google Scholar Articles by Drisdel, R. C. Articles by Green, W. N. Search for Related Content PubMed PubMed Citation Articles by Drisdel, R. C. Articles by Green, W. N.

 Previous Article  |  Next Article 

The Journal of Neuroscience, January 1, 2000, 20(1):133-139

Neuronal -Bungarotoxin Receptors Are 7 Subunit Homomers

Renaldo C. Drisdel and William N. Green

Department of Neurobiology, Pharmacology, and Physiology, University of Chicago, Chicago, Illinois 60637

Nicotinic acetylcholine receptors in the nervous system are heterogeneous with distinct pharmacological and functional properties resulting from differences in post-translational processing and subunit composition. Because of nicotinic receptor diversity, receptor purification and biochemical characterization have been difficult, and the precise subunit composition of each receptor subtype is poorly characterized. Evidence is presented that -bungarotoxin (Bgt)-binding nicotinic receptors found in pheochromocytoma 12 (PC12) cells are pentamers composed solely of 7 subunits. Metabolically labeled, affinity-purified Bgt receptors (BgtRs) consisted of a single 55 kDa band on SDS gels, which was recognized by anti-7 antibodies on immunoblots. Isoelectric focusing separated the 55 kDa band into multiple spots, all recognized by anti-7 antibodies and, therefore, each a differentially processed 7 subunit. Cell-surface BgtR subunits, cross-linked to each other and ¹²⁵I-Bgt, migrated on gels as a ladder of five bands with each band a multiple of an 7 subunit monomer. Similar characteristics of BgtRs from rat brain suggest that they, like PC12 BgtRs, are 7 pentamers containing differentially processed 7 subunits.

Key words: -bungarotoxin; nicotine; neuronal nicotinic receptors; PC12 cells; rat brain; protein structure; post-translational modification

---

Copyright © 2000 Society for Neuroscience  0270-6474/0/201133-07$05.00/0

This article has been cited by other articles:

				K. Maouche, M. Polette, T. Jolly, K. Medjber, I. Cloez-Tayarani, J.-P. Changeux, H. Burlet, C. Terryn, C. Coraux, J.-M. Zahm, et al. {alpha}7 Nicotinic Acetylcholine Receptor Regulates Airway Epithelium Differentiation by Controlling Basal Cell Proliferation Am. J. Pathol., November 1, 2009; 175(5): 1868 - 1882. [Abstract] [Full Text] [PDF]

				E. X. Albuquerque, E. F. R. Pereira, M. Alkondon, and S. W. Rogers Mammalian Nicotinic Acetylcholine Receptors: From Structure to Function Physiol Rev, January 1, 2009; 89(1): 73 - 120. [Abstract] [Full Text] [PDF]

				R. C. Drisdel, D. Sharp, T. Henderson, T. G. Hales, and W. N. Green High Affinity Binding of Epibatidine to Serotonin Type 3 Receptors J. Biol. Chem., April 11, 2008; 283(15): 9659 - 9665. [Abstract] [Full Text] [PDF]

				H. Walsh, A. P. Govind, R. Mastro, J. C. Hoda, D. Bertrand, Y. Vallejo, and W. N. Green Up-regulation of Nicotinic Receptors by Nicotine Varies with Receptor Subtype J. Biol. Chem., March 7, 2008; 283(10): 6022 - 6032. [Abstract] [Full Text] [PDF]

				J. Zhang and D. K. Berg Reversible inhibition of GABAA receptors by {alpha}7-containing nicotinic receptors on the vertebrate postsynaptic neurons J. Physiol., March 15, 2007; 579(3): 753 - 763. [Abstract] [Full Text] [PDF]

				C. Bray, J.-H. Son, and S. Meizel Acetylcholine causes an increase of intracellular calcium in human sperm Mol. Hum. Reprod., December 1, 2005; 11(12): 881 - 889. [Abstract] [Full Text] [PDF]

				S. J. Lansdell, V. J. Gee, P. C. Harkness, A. I. Doward, E. R. Baker, A. J. Gibb, and N. S. Millar RIC-3 Enhances Functional Expression of Multiple Nicotinic Acetylcholine Receptor Subtypes in Mammalian Cells Mol. Pharmacol., November 1, 2005; 68(5): 1431 - 1438. [Abstract] [Full Text] [PDF]

				E. Charpantier, A. Wiesner, K.-H. Huh, R. Ogier, J.-C. Hoda, G. Allaman, M. Raggenbass, D. Feuerbach, D. Bertrand, and C. Fuhrer {alpha}7 Neuronal Nicotinic Acetylcholine Receptors Are Negatively Regulated by Tyrosine Phosphorylation and Src-Family Kinases J. Neurosci., October 26, 2005; 25(43): 9836 - 9849. [Abstract] [Full Text] [PDF]

				C. Bray, J.-H. Son, P. Kumar, and S. Meizel Mice Deficient in CHRNA7, a Subunit of the Nicotinic Acetylcholine Receptor, Produce Sperm with Impaired Motility Biol Reprod, October 1, 2005; 73(4): 807 - 814. [Abstract] [Full Text] [PDF]

				A. L. Obaid, M. E. Nelson, J. Lindstrom, and B. M. Salzberg Optical studies of nicotinic acetylcholine receptor subtypes in the guinea-pig enteric nervous system J. Exp. Biol., August 1, 2005; 208(15): 2981 - 3001. [Abstract] [Full Text] [PDF]

				D. Fayuk and J. L Yakel Ca2+ permeability of nicotinic acetylcholine receptors in rat hippocampal CA1 interneurones J. Physiol., August 1, 2005; 566(3): 759 - 768. [Abstract] [Full Text] [PDF]

				D. Touroutine, R. M. Fox, S. E. Von Stetina, A. Burdina, D. M. Miller III, and J. E. Richmond acr-16 Encodes an Essential Subunit of the Levamisole-resistant Nicotinic Receptor at the Caenorhabditis elegans Neuromuscular Junction J. Biol. Chem., July 22, 2005; 280(29): 27013 - 27021. [Abstract] [Full Text] [PDF]

				P. Kumar and S. Meizel Nicotinic Acetylcholine Receptor Subunits and Associated Proteins in Human Sperm J. Biol. Chem., July 8, 2005; 280(27): 25928 - 25935. [Abstract] [Full Text] [PDF]

				J. Deck, S. Bibevski, T. Gnecchi-Ruscone, V. Bellina, N. Montano, and M. E. Dunlap {alpha}7-Nicotinic acetylcholine receptor subunit is not required for parasympathetic control of the heart in the mouse Physiol Genomics, June 16, 2005; 22(1): 86 - 92. [Abstract] [Full Text] [PDF]

				R. C. Drisdel, E. Manzana, and W. N. Green The Role of Palmitoylation in Functional Expression of Nicotinic {alpha}7 Receptors J. Neurosci., November 17, 2004; 24(46): 10502 - 10510. [Abstract] [Full Text] [PDF]

				V. Avramopoulou, A. Mamalaki, and S. J. Tzartos Soluble, Oligomeric, and Ligand-binding Extracellular Domain of the Human {alpha}7 Acetylcholine Receptor Expressed in Yeast: REPLACEMENT OF THE HYDROPHOBIC CYSTEINE LOOP BY THE HYDROPHILIC LOOP OF THE ACh-BINDING PROTEIN ENHANCES PROTEIN SOLUBILITY J. Biol. Chem., September 10, 2004; 279(37): 38287 - 38293. [Abstract] [Full Text] [PDF]

				E. G. Severance, H. Zhang, Y. Cruz, S. Pakhlevaniants, S. H. Hadley, J. Amin, L. Wecker, C. Reed, and J. Cuevas The {alpha}7 Nicotinic Acetylcholine Receptor Subunit Exists in Two Isoforms that Contribute to Functional Ligand-Gated Ion Channels Mol. Pharmacol., September 1, 2004; 66(3): 420 - 429. [Abstract] [Full Text] [PDF]

				E. R. Baker, R. Zwart, E. Sher, and N. S. Millar Pharmacological Properties of {alpha}9{alpha}10 Nicotinic Acetylcholine Receptors Revealed by Heterologous Expression of Subunit Chimeras Mol. Pharmacol., February 1, 2004; 65(2): 453 - 460. [Abstract] [Full Text] [PDF]

				J. Oshikawa, Y. Toya, T. Fujita, M. Egawa, J. Kawabe, S. Umemura, and Y. Ishikawa Nicotinic acetylcholine receptor {alpha}7 regulates cAMP signal within lipid rafts Am J Physiol Cell Physiol, September 1, 2003; 285(3): C567 - C574. [Abstract] [Full Text] [PDF]

				D. Everhart, E. Reiller, A. Mirzoian, J. M. McIntosh, A. Malhotra, and C. W. Luetje Identification of Residues That Confer {alpha}-Conotoxin-PnIA Sensitivity on the {alpha}3 Subunit of Neuronal Nicotinic Acetylcholine Receptors J. Pharmacol. Exp. Ther., August 1, 2003; 306(2): 664 - 670. [Abstract] [Full Text] [PDF]

				H. Tsuneki, R. Salas, and J. A Dani Mouse muscle denervation increases expression of an {alpha}7 nicotinic receptor with unusual pharmacology J. Physiol., February 15, 2003; 547(1): 169 - 179. [Abstract] [Full Text] [PDF]

				C. L. Brumwell, J. L. Johnson, and M. H. Jacob Extrasynaptic alpha 7-Nicotinic Acetylcholine Receptor Expression in Developing Neurons Is Regulated by Inputs, Targets, and Activity J. Neurosci., September 15, 2002; 22(18): 8101 - 8109. [Abstract] [Full Text] [PDF]

				A. Mirzoian and C. W. Luetje Modulation of Neuronal Nicotinic Acetylcholine Receptors by Mercury J. Pharmacol. Exp. Ther., August 1, 2002; 302(2): 560 - 567. [Abstract] [Full Text] [PDF]

				P. Flood and K. M. Coates Sensitivity of the {alpha}7 Nicotinic Acetylcholine Receptor to Isoflurane May Depend on Receptor Inactivation Anesth. Analg., July 1, 2002; 95(1): 83 - 87. [Abstract] [Full Text] [PDF]

				S. S Khiroug, P. C Harkness, P. W Lamb, S. N Sudweeks, L. Khiroug, N. S Millar, and J. L Yakel Rat nicotinic ACh receptor {alpha}7 and {beta}2 subunits co-assemble to form functional heteromeric nicotinic receptor channels J. Physiol., April 15, 2002; 540(2): 425 - 434. [Abstract] [Full Text] [PDF]

				M. Grauso, R. A. Reenan, E. Culetto, and D. B. Sattelle Novel Putative Nicotinic Acetylcholine Receptor Subunit Genes, D{alpha}5, D{alpha}6 and D{alpha}7, in Drosophila melanogaster Identify a New and Highly Conserved Target of Adenosine Deaminase Acting on RNA-Mediated A-to-I Pre-mRNA Editing Genetics, April 1, 2002; 160(4): 1519 - 1533. [Abstract] [Full Text] [PDF]

				M. J. Parker, S. C. Harvey, and C. W. Luetje Determinants of Agonist Binding Affinity on Neuronal Nicotinic Receptor beta Subunits J. Pharmacol. Exp. Ther., October 1, 2001; 299(1): 385 - 391. [Abstract] [Full Text] [PDF]

				M. M. Francis, E. Y. Cheng, G. A. Weiland, and R. E. Oswald Specific Activation of the alpha 7 Nicotinic Acetylcholine Receptor by a Quaternary Analog of Cocaine Mol. Pharmacol., July 1, 2001; 60(1): 71 - 79. [Abstract] [Full Text]

				B. Hsiao, D. Dweck, and C. W. Luetje Subunit-Dependent Modulation of Neuronal Nicotinic Receptors by Zinc J. Neurosci., March 15, 2001; 21(6): 1848 - 1856. [Abstract] [Full Text] [PDF]

				M. E. McNerney, D. Pardi, P. C. Pugh, Q. Nai, and J. F. Margiotta Expression and Channel Properties of alpha -Bungarotoxin-Sensitive Acetylcholine Receptors on Chick Ciliary and Choroid Neurons J Neurophysiol, September 1, 2000; 84(3): 1314 - 1329. [Abstract] [Full Text] [PDF]

				V. B. Aramakis, C. Y. Hsieh, F. M. Leslie, and R. Metherate A Critical Period for Nicotine-Induced Disruption of Synaptic Development in Rat Auditory Cortex J. Neurosci., August 15, 2000; 20(16): 6106 - 6116. [Abstract] [Full Text] [PDF]

				M. K. Temburni, R. C Blitzblau, and M. H Jacob Receptor targeting and heterogeneity at interneuronal nicotinic cholinergic synapses in vivo J. Physiol., May 15, 2000; 525(1): 21 - 29. [Abstract] [Full Text] [PDF]

				K. T. Dineley and J. W. Patrick Amino Acid Determinants of alpha 7 Nicotinic Acetylcholine Receptor Surface Expression J. Biol. Chem., April 28, 2000; 275(18): 13974 - 13985. [Abstract] [Full Text] [PDF]

				S. S Khiroug, P. C Harkness, P. W Lamb, S. N Sudweeks, L. Khiroug, N. S Millar, and J. L Yakel Rat nicotinic ACh receptor {alpha}7 and {beta}2 subunits co-assemble to form functional heteromeric nicotinic receptor channels J. Physiol., April 15, 2002; 540(2): 425 - 434. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help