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The Journal of Neuroscience, June 15, 2000, 20(12):4535-4544

Phosphorylated Syntaxin 1 Is Localized to Discrete Domains Along a Subset of Axons

Davide L. Foletti, Richard Lin, Michael A. F. Finley, and Richard H. Scheller

Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305-5428

Syntaxin 1 is a SNARE protein that plays a central role in synaptic vesicle (SV) exocytosis. We generated an antibody that specifically recognizes a casein kinase II-mediated phosphorylation on serine-14 of syntaxin 1. In this report we show that this phosphorylation occurs in vivo and is developmentally regulated in the rat brain, rising to a level of 40% of the total syntaxin in adult animals. Phosphorylated syntaxin is preferentially associated with SNAP-25 and localizes to discrete domains of the axonal plasma membrane that do not colocalize with pools of synaptic vesicles. These phosphosyntaxin domains may define fusion sites for a novel class of vesicles outside classical active zones.

Key words: syntaxin 1; phosphorylation; casein kinase II; SNAREs; exocytosis; immunohistochemistry

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Copyright © 2000 Society for Neuroscience  0270-6474/00/20124535-10$05.00/0

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