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Previous Article | Next Article
The Journal of Neuroscience, July 15, 2000, 20(14):5276-5282
VMAT-Mediated Changes in Quantal Size and Vesicular Volume
T. L. Colliver1, 2, S. J. Pyott1, M. Achalabun1, and Andrew G. Ewing1, 2 1 Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, and 2 Department of Neuroscience and Anatomy, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033
It has been well established that the volume of secretory vesicles can be modulated. However, we present the first data demonstrating that the amount of transmitter in a vesicle can regulate its volume. Amperometry and transmission electron microscopy have been used to determine that L-3,4-dihydroxyphenylalanine and reserpine increase and decrease, respectively, the volume of single pheochromocytoma cell vesicles as well as their catecholamine content. Because changes in vesicular catecholamine content are tracked by changes in vesicle volume, our results indicate that when quantal size is altered via the vesicular monoamine transporter the concentration of catecholamines within the vesicles remains relatively constant. This previously unidentified cellular response provides new insight into how catecholamines can be packaged in and released from secretory vesicles.
Key words: vesicular monoamine transporter; amperometry; transmission electron microscopy; PC12 cells; quantal size; vesicle volume
Copyright © 2000 Society for Neuroscience 0270-6474/00/20145276-07$05.00/0
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