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The Journal of Neuroscience, July 15, 2000, 20(14):5329-5338
Small GTPases Rac and Rho in the Maintenance of Dendritic Spines and Branches in Hippocampal Pyramidal Neurons
Ann Y. Nakayama1, 2, Matthew B. Harms1, and Liqun Luo1, 2 1 Department of Biological Sciences and 2 Neurosciences Program, Stanford University, Stanford, California 94305-5020
The shape of dendritic trees and the density of dendritic spines can undergo significant changes during the life of a neuron. We report here the function of the small GTPases Rac and Rho in the maintenance of dendritic structures. Maturing pyramidal neurons in rat hippocampal slice culture were biolistically transfected with dominant GTPase mutants. We found that expression of dominant-negative Rac1 results in a progressive elimination of dendritic spines, whereas hyperactivation of RhoA causes a drastic simplification of dendritic branch patterns that is dependent on the activity of a downstream kinase ROCK. Our results suggest that Rac and Rho play distinct functions in regulating dendritic spines and branches and are vital for the maintenance and reorganization of dendritic structures in maturing neurons.
Key words: Rac; Rho; dendritic spines; biolistic transfection; pyramidal neurons; effector domain mutants; ROCK; Y-27632; PSD-95
Copyright © 2000 Society for Neuroscience 0270-6474/00/20145329-10$05.00/0
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