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Previous Article | Next Article
The Journal of Neuroscience, October 15, 2000, 20(20):7602-7613
Dual Roles for c-Jun N-Terminal Kinase in Developmental and Stress Responses in Cerebellar Granule Neurons
Eleanor T. Coffey1, 2, Vesa Hongisto1, 2, Martin Dickens3, Roger J. Davis4, and Michael J. Courtney1, 2, 5 1 Turku Centre for Biotechnology, Åbo Akademi University and University of Turku, BioCity, FIN-20521 Turku, Finland, 2 Department of Biochemistry and Pharmacy, Åbo Akademi University, BioCity, FIN-20521 Turku, Finland, 3 Department of Biochemistry, University of Leicester, Leicester LE17RH, United Kingdom, 4 Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, University of Massachusetts, Worcester, Massachusetts 01655, and 5 A. I. Virtanen Institute, University of Kuopio, FIN-70211 Kuopio, Finland
c-Jun N-terminal kinases (JNKs) typically respond strongly to stress, are implicated in brain development, and are believed to mediate neuronal apoptosis. Surprisingly, however, JNK does not respond characteristically to stress in cultured cerebellar granule (CBG) neurons, a widely exploited CNS model for studies of death and development, despite the regulation of its substrate c-Jun. To understand this anomaly, we characterized JNK regulation in CBG neurons. We find that the specific activity of CBG JNK is elevated considerably above that from neuron-like cell lines (SH-SY5Y, PC12); however, similar elevated activities are found in brain extracts. This activity does not result from cellular stress because the stress-activated protein kinase p38 is not activated. We identify a minor stress-sensitive pool of JNK that translocates with mitogen-activated protein kinase kinase-4 (MKK4) into the nucleus. However, the major pool of total activity is cytoplasmic, residing largely in the neurites, suggesting a non-nuclear role for JNK in neurons. A third JNK pool is colocalized with MKK7 in the nucleus, and specific activities of both increase during neuritogenesis, nuclear JNK activity increasing 10-fold, whereas c-Jun expression and activity decrease. A role for JNK during differentiation is supported by modulation of neuritic architecture after expression of dominant inhibitory regulators of the JNK pathway. Channeling of JNK signaling away from c-Jun during differentiation is consistent with the presence in the nucleus of the JNK/MKK7 scaffold protein JNK-interacting protein, which inhibits JNK-c-Jun interaction. We propose a model in which distinct pools of JNK serve different functions, providing a basis for understanding multifunctional JNK signaling in differentiating neurons.
Key words: cerebellar granule neuron; stress-activated protein kinase; JNK; JIP; MKK4; MKK7; p38; c-Jun; nuclear translocation; neuronal differentiation; dominant negative; neuronal morphology
Copyright © 2000 Society for Neuroscience 0270-6474/00/20207602-12$05.00/0
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