 |
 Previous Article | Next Article 
The Journal of Neuroscience, November 1, 2000, 20(21):8153-8159
Interleukin-1 -Induced Changes in Blood-Brain Barrier
Permeability, Apparent Diffusion Coefficient, and Cerebral Blood Volume
in the Rat Brain: A Magnetic Resonance Study
A. M.
Blamire1,
D. C.
Anthony2,
B.
Rajagopalan1,
N. R.
Sibson1,
V. H.
Perry2, and
P.
Styles1
1 Medical Research Council Biochemical and Clinical
Magnetic Resonance Unit, Department of Biochemistry, University of
Oxford, Oxford OX1 3QU, United Kingdom, and 2 CNS
Inflammation Group, School of Biological Sciences, University of
Southampton, Southampton SO16 7PX, United Kingdom
The cytokine interleukin-1 (IL-1) is implicated in a broad
spectrum of CNS pathologies, in which it is thought to exacerbate neuronal loss. Here, the effects of injecting recombinant rat IL-1
into the striatum of 3-week-old rats were followed noninvasively from 2 to 123 hr using magnetic resonance imaging and spectroscopy. Four hours
after injection of IL-1 (1 ng in 1 µl), cerebral blood volume was
significantly increased, the blood-brain barrier (BBB) became
permeable to intravenously administered contrast agent between 4.5 and
5 hr, and the apparent diffusion coefficient (ADC) of brain water fell
by 6 hr (5.42 ± 0.35 × 10 4 mm2/sec
treated, 7.35 ± 0.77 × 104 mm2/sec
control; p < 0.001). At 24 hr the BBB was again
intact, but the ADC, although partially recovered, remained depressed
at both 24 and 123 hr (p < 0.03). Depleting
the animals of neutrophils before IL-1 injection prevented the BBB
permeability at all time points, but the ADC was still depressed at 6 hr (6.64 ± 0.34 × 104 mm2/sec
treated, 7.49 ± 0.38 × 104 mm2/sec
control; p < 0.005). No changes were seen in brain
metabolites using proton spectroscopy at 6 hr after IL-1.
Intraparenchymal injection of IL-1 caused a neutrophil-dependent
transient increase in BBB permeability. The presence of neutrophils
within the brain parenchyma significantly contributed to the
IL-1-induced changes in cerebral blood volume and the ADC of brain
water. However, IL-1 apparently had a direct effect on the resident
cell populations, which persisted well after all recruited leukocytes
had disappeared. Thus the action of IL-1 alone can give rise to
magnetic resonance imaging-visible changes that are normally
attributed to alterations to cellular homeostasis.
Key words:
interleukin-1; magnetic resonance; diffusion; cerebral
blood volume; neutrophil; depletion
Copyright © 2000 Society for Neuroscience 0270-6474/00/20218153-07$05.00/0
This article has been cited by other articles:
|
|
|
|
 
S. Serres, D. C. Anthony, Y. Jiang, K. A. Broom, S. J. Campbell, D. J. Tyler, S. I. van Kasteren, B. G. Davis, and N. R. Sibson
Systemic Inflammatory Response Reactivates Immune-Mediated Lesions in Rat Brain
J. Neurosci.,
April 15, 2009;
29(15):
4820 - 4828.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. I. van Kasteren, S. J. Campbell, S. Serres, D. C. Anthony, N. R. Sibson, and B. G. Davis
Glyconanoparticles allow pre-symptomatic in vivo imaging of brain disease
PNAS,
January 6, 2009;
106(1):
18 - 23.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. A. Brooks, N. Nametz, R. Charles, and T. P. Davis
Diclofenac Attenuates the Regional Effect of {lambda}-Carrageenan on Blood-Brain Barrier Function and Cytoarchitecture
J. Pharmacol. Exp. Ther.,
May 1, 2008;
325(2):
665 - 673.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Calderon-Garciduenas, A. C. Solt, C. Henriquez-Roldan, R. Torres-Jardon, B. Nuse, L. Herritt, R. Villarreal-Calderon, N. Osnaya, I. Stone, R. Garcia, et al.
Long-term Air Pollution Exposure Is Associated with Neuroinflammation, an Altered Innate Immune Response, Disruption of the Blood-Brain Barrier, Ultrafine Particulate Deposition, and Accumulation of Amyloid {beta}-42 and {alpha}-Synuclein in Children and Young Adults
Toxicol Pathol,
February 1, 2008;
36(2):
289 - 310.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Behling-Kelly, D. McClenahan, K. S. Kim, and C. J. Czuprynski
Viable "Haemophilus somnus" Induces Myosin Light-Chain Kinase-Dependent Decrease in Brain Endothelial Cell Monolayer Resistance
Infect. Immun.,
September 1, 2007;
75(9):
4572 - 4581.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. B. Dimitrijevic, S. M. Stamatovic, R. F. Keep, and A. V. Andjelkovic
Absence of the Chemokine Receptor CCR2 Protects Against Cerebral Ischemia/Reperfusion Injury in Mice
Stroke,
April 1, 2007;
38(4):
1345 - 1353.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Calderon-Garciduenas, M. Franco-Lira, R. Torres-Jardon, C. Henriquez-Roldan, G. Barragan-Mejia, G. Valencia-Salazar, A. Gonzalez-Maciel, R. Reynoso-Robles, R. Villarreal-Calderon, and W. Reed
Pediatric Respiratory and Systemic Effects of Chronic Air Pollution Exposure: Nose, Lung, Heart, and Brain Pathology
Toxicol Pathol,
January 1, 2007;
35(1):
154 - 162.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wuerfel, J. Bellmann-Strobl, P. Brunecker, O. Aktas, H. McFarland, A. Villringer, and F. Zipp
Changes in cerebral perfusion precede plaque formation in multiple sclerosis: a longitudinal perfusion MRI study
Brain,
January 1, 2004;
127(1):
111 - 119.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Stamatovic, R. F. Keep, S. L. Kunkel, and A. V. Andjelkovic
Potential role of MCP-1 in endothelial cell tight junction `opening': signaling via Rho and Rho kinase
J. Cell Sci.,
November 15, 2003;
116(22):
4615 - 4628.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Calderon-Garciduenas, R. R. Maronpot, R. Torres-Jardon, C. Henriquez-Roldan, R. Schoonhoven, H. Acuna-Ayala, A. Villarreal-Calderon, J. Nakamura, R. Fernando, W. Reed, et al.
DNA Damage in Nasal and Brain Tissues of Canines Exposed to Air Pollutants Is Associated with Evidence of Chronic Brain Inflammation and Neurodegeneration
Toxicol Pathol,
August 1, 2003;
31(5):
524 - 538.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. F Lythgoe, N. R Sibson, and N. G Harris
Neuroimaging of animal models of brain disease
Br. Med. Bull.,
March 1, 2003;
65(1):
235 - 257.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. R. Sibson, A. M. Blamire, V. H. Perry, J. Gauldie, P. Styles, and D. C. Anthony
TNF-{alpha} reduces cerebral blood volume and disrupts tissue homeostasis via an endothelin- and TNFR2-dependent pathway
Brain,
November 1, 2002;
125(11):
2446 - 2459.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. A. Newman, S. T. Woolley, P. M. Hughes, N. R. Sibson, D. C. Anthony, and V. H. Perry
T-cell- and macrophage-mediated axon damage in the absence of a CNS-specific immune response: involvement of metalloproteinases
Brain,
November 1, 2001;
124(11):
2203 - 2214.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
