QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (74) Citing Articles via Google Scholar Google Scholar Articles by Dhingra, A. Articles by Vardi, N. Search for Related Content PubMed PubMed Citation Articles by Dhingra, A. Articles by Vardi, N.

 Previous Article  |  Next Article 

The Journal of Neuroscience, December 15, 2000, 20(24):9053-9058

The Light Response of ON Bipolar Neurons Requires G_o

Anuradha Dhingra¹, Arkady Lyubarsky², Meisheng Jiang³, Edward N. Pugh Jr², Lutz Birnbaumer³, Peter Sterling¹, and Noga Vardi¹

Departments of ¹ Neuroscience and ² Ophthalmology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, and ³ Department of Anesthesiology, University of California at Los Angeles, Los Angeles, California 90024

ON bipolar neurons in retina detect the glutamate released by rods and cones via metabotropic glutamate receptor 6 (mGluR6), whose cascade is unknown. The trimeric G-protein G_o might mediate this cascade because it colocalizes with mGluR6. To test this, we studied the retina in mice negative for the  subunit of G_o (G_o/). Retinal layering, key cell types, synaptic structure, and mGluR6 expression were all normal, as was the a-wave of the electroretinogram, which represents the rod and cone photocurrents. However, the b-wave of the electroretinogram, both rod- and cone-driven components, was entirely missing. Because the b-wave represents the massed response of ON bipolar cells, its loss in the G_o null mouse establishes that the light response of the ON bipolar cell requires G_o. This represents the first function to be defined in vivo for the  subunit of the most abundant G-protein of the brain .

Key words: G-protein; mGluR6; metabotropic glutamate receptor; retina; bipolar cells; electroretinogram; rod bipolar cells; second messenger cascade

---

Copyright © 2000 Society for Neuroscience  0270-6474/00/20249053-06$05.00/0

This article has been cited by other articles:

				D. Beqollari, M. J. Betzenhauser, and P. J. Kammermeier Altered G-Protein Coupling in an mGluR6 Point Mutant Associated with Congenital Stationary Night Blindness Mol. Pharmacol., November 1, 2009; 76(5): 992 - 997. [Abstract] [Full Text] [PDF]

				T. M. Schmidt Role of Melastatin-Related Transient Receptor Potential Channel TRPM1 in the Retina: Clues from Horses and Mice J. Neurosci., September 23, 2009; 29(38): 11720 - 11722. [Full Text] [PDF]

				Y. Cao, I. Masuho, H. Okawa, K. Xie, J. Asami, P. J. Kammermeier, D. M. Maddox, T. Furukawa, T. Inoue, A. P. Sampath, et al. Retina-Specific GTPase Accelerator RGS11/G{beta}5S/R9AP Is a Constitutive Heterotrimer Selectively Targeted to mGluR6 in ON-Bipolar Neurons J. Neurosci., July 22, 2009; 29(29): 9301 - 9313. [Abstract] [Full Text] [PDF]

				D. K. Mojumder, Y. Qian, and T. G. Wensel Two R7 Regulator of G-Protein Signaling Proteins Shape Retinal Bipolar Cell Signaling J. Neurosci., June 17, 2009; 29(24): 7753 - 7765. [Abstract] [Full Text] [PDF]

				Y. Nakajima, M. Moriyama, M. Hattori, N. Minato, and S. Nakanishi Isolation of ON Bipolar Cell Genes via hrGFP-coupled Cell Enrichment Using the mGluR6 Promoter J. Biochem., June 1, 2009; 145(6): 811 - 818. [Abstract] [Full Text] [PDF]

				Y. Shen, J. A. Heimel, M. Kamermans, N. S. Peachey, R. G. Gregg, and S. Nawy A Transient Receptor Potential-Like Channel Mediates Synaptic Transmission in Rod Bipolar Cells J. Neurosci., May 13, 2009; 29(19): 6088 - 6093. [Abstract] [Full Text] [PDF]

				D. Specht, S.-B. Wu, P. Turner, P. Dearden, F. Koentgen, U. Wolfrum, M. Maw, J. H. Brandstatter, and S. tom Dieck Effects of Presynaptic Mutations on a Postsynaptic Cacna1s Calcium Channel Colocalized with mGluR6 at Mouse Photoreceptor Ribbon Synapses Invest. Ophthalmol. Vis. Sci., February 1, 2009; 50(2): 505 - 515. [Abstract] [Full Text] [PDF]

				Y. Cao, H. Song, H. Okawa, A. P. Sampath, M. Sokolov, and K. A. Martemyanov Targeting of RGS7/G{beta}5 to the Dendritic Tips of ON-Bipolar Cells Is Independent of Its Association with Membrane Anchor R7BP J. Neurosci., October 8, 2008; 28(41): 10443 - 10449. [Abstract] [Full Text] [PDF]

				M. A. McCall and R. G. Gregg Comparisons of structural and functional abnormalities in mouse b-wave mutants J. Physiol., September 15, 2008; 586(18): 4385 - 4392. [Abstract] [Full Text] [PDF]

				D. M. Maddox, K. A. Vessey, G. L. Yarbrough, B. M. Invergo, D. R. Cantrell, S. Inayat, V. Balannik, W. L. Hicks, N. L. Hawes, S. Byers, et al. Allelic variance between GRM6 mutants, Grm6nob3 and Grm6nob4 results in differences in retinal ganglion cell visual responses J. Physiol., September 15, 2008; 586(18): 4409 - 4424. [Abstract] [Full Text] [PDF]

				Y. Xu, P. Sulaiman, R. M. Feddersen, J. Liu, R. G. Smith, and N. Vardi Retinal ON Bipolar Cells Express a New PCP2 Splice Variant That Accelerates the Light Response J. Neurosci., September 3, 2008; 28(36): 8873 - 8884. [Abstract] [Full Text] [PDF]

				A. Rao, R. Dallman, S. Henderson, and C.-K. Chen G 5 Is Required for Normal Light Responses and Morphology of Retinal ON-Bipolar Cells J. Neurosci., December 19, 2007; 27(51): 14199 - 14204. [Abstract] [Full Text] [PDF]

				C. Koike, A. Nishida, S. Ueno, H. Saito, R. Sanuki, S. Sato, A. Furukawa, S. Aizawa, I. Matsuo, N. Suzuki, et al. Functional Roles of Otx2 Transcription Factor in Postnatal Mouse Retinal Development Mol. Cell. Biol., December 1, 2007; 27(23): 8318 - 8329. [Abstract] [Full Text] [PDF]

				R. G. Gregg, M. Kamermans, J. Klooster, P. D. Lukasiewicz, N. S. Peachey, K. A. Vessey, and M. A. McCall Nyctalopin Expression in Retinal Bipolar Cells Restores Visual Function in a Mouse Model of Complete X-Linked Congenital Stationary Night Blindness J Neurophysiol, November 1, 2007; 98(5): 3023 - 3033. [Abstract] [Full Text] [PDF]

				Y. Tsukamoto, K. Morigiwa, M. Ishii, M. Takao, K. Iwatsuki, S. Nakanishi, and Y. Fukuda A Novel Connection between Rods and ON Cone Bipolar Cells Revealed by Ectopic Metabotropic Glutamate Receptor 7 (mGluR7) in mGluR6-Deficient Mouse Retinas J. Neurosci., June 6, 2007; 27(23): 6261 - 6267. [Abstract] [Full Text] [PDF]

				J. Yang, B. Pawlyk, X.-H. Wen, M. Adamian, M. Soloviev, N. Michaud, Y. Zhao, M. A. Sandberg, C. L. Makino, and T. Li Mpp4 is required for proper localization of plasma membrane calcium ATPases and maintenance of calcium homeostasis at the rod photoreceptor synaptic terminals Hum. Mol. Genet., May 1, 2007; 16(9): 1017 - 1029. [Abstract] [Full Text] [PDF]

				S. Valleix, B. Nedelec, F. Rigaudiere, P. Dighiero, Y. Pouliquen, G. Renard, J.-F. Le Gargasson, and M. Delpech H244R VSX1 Is Associated with Selective Cone ON Bipolar Cell Dysfunction and Macular Degeneration in a PPCD Family Invest. Ophthalmol. Vis. Sci., January 1, 2006; 47(1): 48 - 54. [Abstract] [Full Text] [PDF]

				N. Wettschureck and S. Offermanns Mammalian G Proteins and Their Cell Type Specific Functions Physiol Rev, October 1, 2005; 85(4): 1159 - 1204. [Abstract] [Full Text] [PDF]

				S. Winter, I. Brunk, D. J. Walther, M. Holtje, M. Jiang, J.-U. Peter, S. Takamori, R. Jahn, L. Birnbaumer, and G. Ahnert-Hilger G{alpha}o2 Regulates Vesicular Glutamate Transporter Activity by Changing Its Chloride Dependence J. Neurosci., May 4, 2005; 25(18): 4672 - 4680. [Abstract] [Full Text] [PDF]

				N. Hosoi, I. Arai, and M. Tachibana Group III Metabotropic Glutamate Receptors and Exocytosed Protons Inhibit L-Type Calcium Currents in Cones But Not in Rods J. Neurosci., April 20, 2005; 25(16): 4062 - 4072. [Abstract] [Full Text] [PDF]

				P. Koulen, J. Liu, E. Nixon, and C. Madry Interaction between mGluR8 and Calcium Channels in Photoreceptors Is Sensitive to Pertussis Toxin and Occurs Via G Protein {beta}{gamma} Subunit Signaling Invest. Ophthalmol. Vis. Sci., January 1, 2005; 46(1): 287 - 291. [Abstract] [Full Text] [PDF]

				K. Y. Wong, E. D. Cohen, and J. E. Dowling Retinal Bipolar Cell Input Mechanisms in Giant Danio. II. Patch-Clamp Analysis of ON Bipolar Cells J Neurophysiol, January 1, 2005; 93(1): 94 - 107. [Abstract] [Full Text] [PDF]

				A. Dhingra, E. Faurobert, N. Dascal, P. Sterling, and N. Vardi A Retinal-Specific Regulator of G-Protein Signaling Interacts with G{alpha}o and Accelerates an Expressed Metabotropic Glutamate Receptor 6 Cascade J. Neurosci., June 23, 2004; 24(25): 5684 - 5693. [Abstract] [Full Text] [PDF]

				R. L. Chow, B. Volgyi, R. K. Szilard, D. Ng, C. McKerlie, S. A. Bloomfield, D. G. Birch, and R. R. McInnes Control of late off-center cone bipolar cell differentiation and visual signaling by the homeobox gene Vsx1 PNAS, February 10, 2004; 101(6): 1754 - 1759. [Abstract] [Full Text] [PDF]

				M. E. Pennesi, J.-H. Cho, Z. Yang, S. H. Wu, J. Zhang, S. M. Wu, and M.-J. Tsai BETA2/NeuroD1 Null Mice: A New Model for Transcription Factor-Dependent Photoreceptor Degeneration J. Neurosci., January 15, 2003; 23(2): 453 - 461. [Abstract] [Full Text] [PDF]

				R. G. Gregg, S. Mukhopadhyay, S. I. Candille, S. L. Ball, M. T. Pardue, M. A. McCall, and N. S. Peachey Identification of the Gene and the Mutation Responsible for the Mouse nob Phenotype Invest. Ophthalmol. Vis. Sci., January 1, 2003; 44(1): 378 - 384. [Abstract] [Full Text] [PDF]

				J. Snellman and S. Nawy Regulation of the Retinal Bipolar Cell mGluR6 Pathway by Calcineurin J Neurophysiol, September 1, 2002; 88(3): 1088 - 1096. [Abstract] [Full Text] [PDF]

				R A Shiells and G Falk Potentiation of 'on' bipolar cell flash responses by dim background light and cGMP in dogfish retinal slices J. Physiol., July 1, 2002; 542(1): 211 - 220. [Abstract] [Full Text] [PDF]

				A. Dhingra, M. Jiang, T.-L. Wang, A. Lyubarsky, A. Savchenko, T. Bar-Yehuda, P. Sterling, L. Birnbaumer, and N. Vardi Light Response of Retinal ON Bipolar Cells Requires a Specific Splice Variant of Galpha o J. Neurosci., June 15, 2002; 22(12): 4878 - 4884. [Abstract] [Full Text] [PDF]

				S. L. Ball, P. A. Powers, H.-S. Shin, C. W. Morgans, N. S. Peachey, and R. G. Gregg Role of the {beta}2 Subunit of Voltage-Dependent Calcium Channels in the Retinal Outer Plexiform Layer Invest. Ophthalmol. Vis. Sci., May 1, 2002; 43(5): 1595 - 1603. [Abstract] [Full Text] [PDF]

				Genetically Modified Animals in Endocrinology Endocr. Rev., April 1, 2002; 23(2): 276 - 278. [Full Text] [PDF]

				V. R. Krishna, K. R. Alexander, and N. S. Peachey Temporal Properties of the Mouse Cone Electroretinogram J Neurophysiol, January 1, 2002; 87(1): 42 - 48. [Abstract] [Full Text] [PDF]

				M. Jiang, K. Spicher, G. Boulay, Y. Wang, and L. Birnbaumer Most central nervous system D2 dopamine receptors are coupled to their effectors by Go PNAS, March 1, 2001; (2001) 51632598. [Abstract] [Full Text]

				M. Jiang, K. Spicher, G. Boulay, Y. Wang, and L. Birnbaumer Most central nervous system D2 dopamine receptors are coupled to their effectors by Go PNAS, March 13, 2001; 98(6): 3577 - 3582. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help