QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Belyantseva, I. A. Articles by Kachar, B. Search for Related Content PubMed PubMed Citation Articles by Belyantseva, I. A. Articles by Kachar, B.

 Previous Article  |  Next Article 

The Journal of Neuroscience, 0000, 20:RC116:1-5

RAPID COMMUNICATION
Expression and Localization of Prestin and the Sugar Transporter GLUT-5 during Development of Electromotility in Cochlear Outer Hair Cells

Inna A. Belyantseva¹, Henry J. Adler¹, Rui Curi², Gregory I. Frolenkov¹, and Bechara Kachar¹

¹ Section on Structural Cell Biology, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, Maryland 20892, and ² Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Brazil

Electromotility, i.e., the ability of cochlear outer hair cells (OHCs) to contract and elongate at acoustic frequencies, is presumed to depend on the voltage-driven conformational changes of "motor" proteins present in the OHC lateral plasma membrane. Recently, two membrane proteins have been proposed as candidates for the OHC motor. A sugar transporter, GLUT-5, was proposed based on its localization in the OHCs and on the observation that sugar transport alters the voltage sensitivity of the OHC motor mechanism. Another candidate, "prestin," was identified from a subtracted OHC cDNA library and shown to impart voltage-driven shape changes to transfected cultured cells. We used antibodies specific for these two proteins to show that they are highly expressed in the lateral membrane of OHCs. We also compared the postnatal expression patterns of these proteins with the development of electromotility in OHCs of the apical turn of the rat organ of Corti. The patch-clamp recording of transient charge movement associated with electromotility indicates that half of the maximal expression of the motor protein occurs at postnatal day 9. Prestin incorporation in the plasma membrane begins from postnatal day 0 and increases progressively in a time course coinciding with that of electromotility. GLUT-5 is not incorporated into the lateral plasma membrane of apical OHCs until postnatal day 15. Our results suggest that, although GLUT-5 may be involved in the control of electromotility, prestin is likely to be a fundamental component of the OHC membrane motor mechanism.

Key words: mechanosensory transduction; unconventional cell motility; motor protein; organ of Corti; postnatal development; voltage-dependent capacitance

---

Copyright © 0000 Society for Neuroscience  0270-6474/00/$05.00/0

This article has been cited by other articles:

				A. Lelli, Y. Asai, A. Forge, J. R. Holt, and G. S. G. Geleoc Tonotopic Gradient in the Developmental Acquisition of Sensory Transduction in Outer Hair Cells of the Mouse Cochlea J Neurophysiol, June 1, 2009; 101(6): 2961 - 2973. [Abstract] [Full Text] [PDF]

				K. Legendre, S. Safieddine, P. Kussel-Andermann, C. Petit, and A. El-Amraoui {alpha}II-{beta}V spectrin bridges the plasma membrane and cortical lattice in the lateral wall of the auditory outer hair cells J. Cell Sci., October 15, 2008; 121(20): 3347 - 3356. [Abstract] [Full Text] [PDF]

				G. Li, J. Wang, S. J. Rossiter, G. Jones, J. A. Cotton, and S. Zhang The hearing gene Prestin reunites echolocating bats PNAS, September 16, 2008; 105(37): 13959 - 13964. [Abstract] [Full Text] [PDF]

				J. Ashmore Cochlear Outer Hair Cell Motility Physiol Rev, January 1, 2008; 88(1): 173 - 210. [Abstract] [Full Text] [PDF]

				L. Rajagopalan, J. N. Greeson, A. Xia, H. Liu, A. Sturm, R. M. Raphael, A. L. Davidson, J. S. Oghalai, F. A. Pereira, and W. E. Brownell Tuning of the Outer Hair Cell Motor by Membrane Cholesterol J. Biol. Chem., December 14, 2007; 282(50): 36659 - 36670. [Abstract] [Full Text] [PDF]

				H. Yagi, H. Tokano, M. Maeda, T. Takabayashi, T. Nagano, H. Kiyama, S. Fujieda, K. Kitamura, and M. Sato Vlgr1 is required for proper stereocilia maturation of cochlear hair cells. Genes Cells, February 1, 2007; 12(2): 235 - 250. [Abstract] [Full Text] [PDF]

				G. I. Frolenkov Regulation of electromotility in the cochlear outer hair cell J. Physiol., October 1, 2006; 576(1): 43 - 48. [Abstract] [Full Text] [PDF]

				Z. M. Ahmed, S. Riazuddin, J. Ahmad, S. L. Bernstein, Y. Guo, M. F. Sabar, P. Sieving, S. Riazuddin, A. J. Griffith, T. B. Friedman, et al. PCDH15 is expressed in the neurosensory epithelium of the eye and ear and mutant alleles are responsible for both USH1F and DFNB23 Hum. Mol. Genet., December 15, 2003; 12(24): 3215 - 3223. [Abstract] [Full Text] [PDF]

				T. Ben-Yosef, I. A. Belyantseva, T. L. Saunders, E. D. Hughes, K. Kawamoto, C. M. Van Itallie, L. A. Beyer, K. Halsey, D. J. Gardner, E. R. Wilcox, et al. Claudin 14 knockout mice, a model for autosomal recessive deafness DFNB29, are deaf due to cochlear hair cell degeneration Hum. Mol. Genet., August 15, 2003; 12(16): 2049 - 2061. [Abstract] [Full Text] [PDF]

				F. Di Palma, I. A. Belyantseva, H. J. Kim, T. F. Vogt, B. Kachar, and K. Noben-Trauth From the Cover: Mutations in Mcoln3 associated with deafness and pigmentation defects in varitint-waddler (Va) mice PNAS, November 12, 2002; 99(23): 14994 - 14999. [Abstract] [Full Text] [PDF]

				J. Santos-Sacchi, W. Shen, J. Zheng, and P. Dallos Effects of membrane potential and tension on prestin, the outer hair cell lateral membrane motor protin J. Physiol., March 15, 2001; 531(3): 661 - 666. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help