 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
The Journal of Neuroscience, March 1, 2000, 20(5):1800-1808
Imaging Extracellular Waves of Glutamate during Calcium Signaling in Cultured Astrocytes
Barbara Innocenti, Vladimir Parpura, and Philip G. Haydon Roy J. Carver Laboratory for Ultrahigh Resolution Biological Microscopy, Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011
A growing body of evidence proposes that glial cells have the potential to play a role as modulators of neuronal activity and synaptic transmission by releasing the neurotransmitter glutamate (Araque et al., 1999). We explore the spatial nature of glutamate release from astrocytes with an enzyme-linked assay system and CCD imaging technology. In the presence of glutamate, L-glutamic dehydrogenase (GDH) reduces NAD+ to NADH, a product that fluoresces when excited with UV light. Theoretically, provided that GDH and NAD+ are present in the bathing saline, the release of glutamate from stimulated astrocytes can be optically detected by monitoring the accumulation of NADH. Indeed, stimuli that induce a wave of elevated calcium among astrocytes produced a corresponding spread of extracellular NADH fluorescence. Treatment of cultures either with thapsigargin, to deplete internal calcium stores, or with the membrane-permeant calcium chelator BAPTA AM significantly decreased the accumulation of NADH, demonstrating that this fluorometric assay effectively monitors calcium-dependent glutamate release. With a temporal resolution of 500 msec and spatial resolution of ~20 µm, discrete regions of glutamate release were not reliably resolved. The wave of glutamate release that underlies the NADH fluorescence propagated at an average speed of ~26 µm/sec, correlating with the rate of calcium wave progression (10-30 µm/sec), and caused a localized accumulation of glutamate in the range of 1-100 µM. Further analysis of the fluorescence accumulation clearly demonstrated that glutamate is released in a regenerative manner, with subsequent cells that are involved in the calcium wave releasing additional glutamate.
Key words: calcium waves; L-glutamic dehydrogenase (GDH); glutamate release; regenerative glutamate waves; astrocyte signaling; glutamate physiology
Copyright © 2000 Society for Neuroscience 0270-6474/00/2051800-09$05.00/0
This article has been cited by other articles:
L. Pawson, L. T. Prestia, G. K. Mahoney, B. Guclu, P. J. Cox, and A. K. Pack
GABAergic/Glutamatergic-Glial/Neuronal Interaction Contributes to Rapid Adaptation in Pacinian Corpuscles
J. Neurosci., March 4, 2009; 29(9): 2695 - 2705.
[Abstract] [Full Text] [PDF]
P. Ramamoorthy and M. D. Whim
Trafficking and Fusion of Neuropeptide Y-Containing Dense-Core Granules in Astrocytes
J. Neurosci., December 17, 2008; 28(51): 13815 - 13827.
[Abstract] [Full Text] [PDF]
R. C. Reyes and V. Parpura
Mitochondria Modulate Ca2+-Dependent Glutamate Release from Rat Cortical Astrocytes
J. Neurosci., September 24, 2008; 28(39): 9682 - 9691.
[Abstract] [Full Text] [PDF]
S. A. Hires, Y. Zhu, and R. Y. Tsien
Optical measurement of synaptic glutamate spillover and reuptake by linker optimized glutamate-sensitive fluorescent reporters
PNAS, March 18, 2008; 105(11): 4411 - 4416.
[Abstract] [Full Text] [PDF]
G. Burnstock
Physiology and Pathophysiology of Purinergic Neurotransmission
Physiol Rev, April 1, 2007; 87(2): 659 - 797.
[Abstract] [Full Text] [PDF]
P. G. Haydon and G. Carmignoto
Astrocyte control of synaptic transmission and neurovascular coupling.
Physiol Rev, July 1, 2006; 86(3): 1009 - 1031.
[Abstract] [Full Text] [PDF]
R. Zur Nieden and J. W. Deitmer
The Role of Metabotropic Glutamate Receptors for the Generation of Calcium Oscillations in Rat Hippocampal Astrocytes In Situ
Cereb Cortex, May 1, 2006; 16(5): 676 - 687.
[Abstract] [Full Text] [PDF]
B. Haas, C. G. Schipke, O. Peters, G. Sohl, K. Willecke, and H. Kettenmann
Activity-dependent ATP-waves in the Mouse Neocortex are Independent from Astrocytic Calcium Waves
Cereb Cortex, February 1, 2006; 16(2): 237 - 246.
[Abstract] [Full Text] [PDF]
A. Lomniczi, A. Cornea, M. E. Costa, and S. R. Ojeda
Hypothalamic Tumor Necrosis Factor-{alpha} Converting Enzyme Mediates Excitatory Amino Acid-Dependent Neuron-to-Glia Signaling in the Neuroendocrine Brain
J. Neurosci., January 4, 2006; 26(1): 51 - 62.
[Abstract] [Full Text] [PDF]
C. Rose, W. Kresse, and H. Kettenmann
Acute Insult of Ammonia Leads to Calcium-dependent Glutamate Release from Cultured Astrocytes, an Effect of pH
J. Biol. Chem., June 3, 2005; 280(22): 20937 - 20944.
[Abstract] [Full Text] [PDF]
J.-P. Mothet, L. Pollegioni, G. Ouanounou, M. Martineau, P. Fossier, and G. Baux
Glutamate receptor activation triggers a calcium-dependent and SNARE protein-dependent release of the gliotransmitter D-serine
PNAS, April 12, 2005; 102(15): 5606 - 5611.
[Abstract] [Full Text] [PDF]
T. Fellin and G. Carmignoto
Neurone-to-astrocyte signalling in the brain represents a distinct multifunctional unit
J. Physiol., August 15, 2004; 559(1): 3 - 15.
[Abstract] [Full Text] [PDF]
J. Kleeberg, G. C. Petzold, S. Major, U. Dirnagl, and J. P. Dreier
ET-1 induces cortical spreading depression via activation of the ETA receptor/phospholipase C pathway in vivo
Am J Physiol Heart Circ Physiol, April 1, 2004; 286(4): H1339 - H1346.
[Abstract] [Full Text] [PDF]
Q. Zhang, T. Pangrsic, M. Kreft, M. Krzan, N. Li, J.-Y. Sul, M. Halassa, E. Van Bockstaele, R. Zorec, and P. G. Haydon
Fusion-related Release of Glutamate from Astrocytes
J. Biol. Chem., March 26, 2004; 279(13): 12724 - 12733.
[Abstract] [Full Text] [PDF]
V. Montana, Y. Ni, V. Sunjara, X. Hua, and V. Parpura
Vesicular Glutamate Transporter-Dependent Glutamate Release from Astrocytes
J. Neurosci., March 17, 2004; 24(11): 2633 - 2642.
[Abstract] [Full Text] [PDF]
N. Li, J.-Y. Sul, and P. G. Haydon
A Calcium-Induced Calcium Influx Factor, Nitric Oxide, Modulates the Refilling of Calcium Stores in Astrocytes
J. Neurosci., November 12, 2003; 23(32): 10302 - 10310.
[Abstract] [Full Text] [PDF]
E. R. Lazarowski, R. C. Boucher, and T. K. Harden
Mechanisms of Release of Nucleotides and Integration of Their Action as P2X- and P2Y-Receptor Activating Molecules
Mol. Pharmacol., October 1, 2003; 64(4): 785 - 795.
[Full Text] [PDF]
F. Aguado, J. F. Espinosa-Parrilla, M. A. Carmona, and E. Soriano
Neuronal Activity Regulates Correlated Network Properties of Spontaneous Calcium Transients in Astrocytes In Situ
J. Neurosci., November 1, 2002; 22(21): 9430 - 9444.
[Abstract] [Full Text] [PDF]
R. D. Fields and B. Stevens-Graham
NEUROSCIENCE: New Insights into Neuron-Glia Communication
Science, October 18, 2002; 298(5593): 556 - 562.
[Abstract] [Full Text] [PDF]
C. E. Stout, J. L. Costantin, C. C. G. Naus, and A. C. Charles
Intercellular Calcium Signaling in Astrocytes via ATP Release through Connexin Hemichannels
J. Biol. Chem., March 15, 2002; 277(12): 10482 - 10488.
[Abstract] [Full Text] [PDF]
H. Muyderman, M. Angehagen, M. Sandberg, U. Bjorklund, T. Olsson, E. Hansson, and M. Nilsson
alpha 1-Adrenergic Modulation of Metabotropic Glutamate Receptor-induced Calcium Oscillations and Glutamate Release in Astrocytes
J. Biol. Chem., November 30, 2001; 276(49): 46504 - 46514.
[Abstract] [Full Text] [PDF]
M. Mazzanti, J.-Y. Sul, and P. G. Haydon
Book Review: Glutamate on Demand: Astrocytes as a Ready Source
Neuroscientist, October 1, 2001; 7(5): 396 - 405.
[Abstract] [PDF]
N. N OSBORNE, J. MELENA, G. CHIDLOW, and J. P M WOOD
A hypothesis to explain ganglion cell death caused by vascular insults at the optic nerve head: possible implication for the treatment of glaucoma
Br J Ophthalmol, October 1, 2001; 85(10): 1252 - 1259.
[Full Text] [PDF]
S. Vesce, P. Bezzi, and A. Volterra
Synaptic Transmission with the Glia
Physiology, August 1, 2001; 16(4): 178 - 184.
[Abstract] [Full Text] [PDF]
C. Verderio and M. Matteoli
ATP Mediates Calcium Signaling Between Astrocytes and Microglial Cells: Modulation by IFN-{{gamma}}
J. Immunol., May 15, 2001; 166(10): 6383 - 6391.
[Abstract] [Full Text] [PDF]
E. A. Newman
Propagation of Intercellular Calcium Waves in Retinal Astrocytes and Muller Cells
J. Neurosci., April 1, 2001; 21(7): 2215 - 2223.
[Abstract] [Full Text] [PDF]
G. Su, D. B. Kintner, and D. Sun
Contribution of Na+-K+-Cl- cotransporter to high-[K+]o- induced swelling and EAA release in astrocytes
Am J Physiol Cell Physiol, May 1, 2002; 282(5): C1136 - C1146.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|