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The Journal of Neuroscience, March 15, 2000, 20(6):2202-2208

Functional Correlation of GABA_A Receptor  Subunits Expression with the Properties of IPSCs in the Developing Thalamus

Masayoshi Okada¹, Kayoko Onodera¹, Catherine Van Renterghem¹, Werner Sieghart², and Tomoyuki Takahashi¹

¹ Department of Neurophysiology, University of Tokyo Faculty of Medicine, Tokyo 113-0033, Japan, and ² Section of Biochemical Psychiatry, University Clinic for Psychiatry, A1090 Vienna, Austria

GABA_A receptor 1 and 2 subunits are expressed differentially with ontogenic period in the brain, but their functional roles are not known. We have recorded GABA_A receptor-mediated IPSCs from laterodorsal (LD) thalamic relay neurons in slices of rat brain at various postnatal ages and found that decay times of evoked IPSCs and spontaneous miniature IPSCs undergo progressive shortening during the first postnatal month. With a similar time course, expression of transcripts and proteins of GABA_A receptor 2 subunit in LD thalamic region declined, being replaced by those of 1 subunit. To further address the causal relationship between  subunits and IPSC decay time kinetics, we have overexpressed GABA_A receptor 1 subunit together with green fluorescent protein in LD thalamic neurons in organotypic culture using recombinant Sindbis virus vectors. Miniature IPSCs recorded from the LD thalamic neurons overexpressed with 1 subunit had significantly faster decay time compared with control expressed with -galactosidase. We conclude that the 2-to-1 subunit switch underlies the developmental speeding in the decay time of GABAergic IPSCs.

Key words: GABA_A receptor; subunit; thalamus; IPSC; postnatal development; patch clamp; Sindbis virus vector

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Copyright © 2000 Society for Neuroscience  0270-6474/00/2062202-07$05.00/0

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