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The Journal of Neuroscience, March 15, 2000, 20(6):2247-2254
Late Retinal Progenitor Cells Show Intrinsic Limitations in the
Production of Cell Types and the Kinetics of Opsin Synthesis
Michael J.
Belliveau,
Tracy L.
Young, and
Constance L.
Cepko
Department of Genetics, and Howard Hughes Medical Institute,
Harvard Medical School, Boston, Massachusetts 02115
The seven major cell classes of the vertebrate neural retina arise
from a pool of multipotent progenitor cells. Several studies suggest a
model of retinal development in which both the environment and the
progenitor cells themselves change over time (Cepko et al., 1996). To
test this model, we used a reaggregate culture system in which a
labeled population of progenitor cells from the postnatal rat retina
were cultured with an excess of embryonic retinal cells. The labeled
cells were then assayed for their cell fate choices and their kinetics
of rod differentiation, as measured by opsin synthesis. The kinetics of
opsin synthesis remained unchanged, but fewer postnatal cells adopted
the rod cell fate when cultured with embryonic cells. There was an
increase in the percentage of bipolar cells produced by postnatal
progenitor cells, indicating a possible respecification of fate. The
increase in bipolar cells could occur even after progenitor cells had
completed their terminal mitoses. These alterations in cell fates
appeared to be caused at least in part by a secreted factor released by
the embryonic cells that requires the LIFR /gp130 complex for
signaling. Finally, although surrounded by 20-fold more embryonic
cells, the postnatal cells did not choose to adopt any fates normally
produced only by embryonic cells.
Key words:
progenitor cell; opsin; retina; bipolar cell; rod
photoreceptor; embryonic retinal cell; rhodopsin kinetics
Copyright © 2000 Society for Neuroscience 0270-6474/00/2062247-08$05.00/0
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