 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
The Journal of Neuroscience, March 15, 2000, 20(6):2275-2286
Application of Neutralizing Antibodies against NI-35/250 Myelin-Associated Neurite Growth Inhibitory Proteins to the Adult Rat Cerebellum Induces Sprouting of Uninjured Purkinje Cell Axons
Annalisa Buffo1, Marta Zagrebelsky1, Andrea B. Huber2, Arne Skerra3, Martin E. Schwab2, Piergiorgio Strata1, and Ferdinando Rossi1 1 Department of Neuroscience and "Rita Levi Montalcini Center for Brain Repair," University of Turin, I-10125 Turin, Italy, 2 Brain Research Institute, University of Zurich, and Federal Institute of Technology, CH-8057 Zurich, Switzerland, and 3 Lehrstuhl für Biologische Chemie, Technische Universität München, D-85350 München, Germany
The myelin-associated proteins NI-35/250 exert a powerful inhibition on axon regeneration, but their function exerted on intact neurons is still unclear. In the adult CNS these proteins are thought to regulate axon growth processes to confine plasticity within restricted regions and to prevent the formation of aberrant connections. We have recently shown that application of neutralizing IN-1 antibody Fab fragment against NI-35/250 proteins to the adult cerebellum induces the expression of injury/growth-associated markers in intact Purkinje cells. Here, we asked whether these cellular modifications are accompanied by growth phenomena of Purkinje neurites. A single intraparenchymal application of IN-1 Fab fragment to the adult cerebellum induces a profuse sprouting of Purkinje axons along their intracortical course. The newly formed processes spread to cover most of the granular layer depth. A significant axon outgrowth is evident 2 d after injection; it tends to increase at 5 and 7 d, but it is almost completely reversed after 1 month. No axonal modifications occur in control Fab-treated cerebella. The IN-1 Fab fragment-induced cellular changes and axon remodeling are essentially reproduced by applying affinity-purified antibody 472 raised against a peptide sequence of the recombinant protein NI-220, thus confirming the specificity of the applied treatments on these myelin-associated molecules. Functional neutralization of NI-35/250 proteins induces outgrowth from uninjured Purkinje neurites in the adult cerebellum. Together with previous observations, this suggests that these molecules regulate axonal plasticity to maintain the proper targeting of terminal arbors within specific gray matter regions.
Key words: sprouting; myelin-associated neurite growth inhibitors; axon growth-associated proteins; axon regeneration; intrinsic determinants; cerebellum
Copyright © 2000 Society for Neuroscience 0270-6474/00/2062275-12$05.00/0
This article has been cited by other articles:
L. Montani, B. Gerrits, P. Gehrig, A. Kempf, L. Dimou, B. Wollscheid, and M. E. Schwab
Neuronal Nogo-A Modulates Growth Cone Motility via Rho-GTP/LIMK1/Cofilin in the Unlesioned Adult Nervous System
J. Biol. Chem., April 17, 2009; 284(16): 10793 - 10807.
[Abstract] [Full Text] [PDF]
D. Fioravante, R.-Y. Liu, and J. H. Byrne
The Ubiquitin-Proteasome System Is Necessary for Long-Term Synaptic Depression in Aplysia
J. Neurosci., October 8, 2008; 28(41): 10245 - 10256.
[Abstract] [Full Text] [PDF]
S. Rossignol, M. Schwab, M. Schwartz, and M. G. Fehlings
Spinal Cord Injury: Time to Move?
J. Neurosci., October 31, 2007; 27(44): 11782 - 11792.
[Abstract] [Full Text] [PDF]
A. Mingorance-Le Meur, B. Zheng, E. Soriano, and J. A. del Rio
Involvement of the Myelin-Associated Inhibitor Nogo-A in Early Cortical Development and Neuronal Maturation
Cereb Cortex, October 1, 2007; 17(10): 2375 - 2386.
[Abstract] [Full Text] [PDF]
A. Jurewicz, M. Matysiak, C. S. Raine, and K. Selmaj
Soluble Nogo-A, an inhibitor of axonal regeneration, as a biomarker for multiple sclerosis
Neurology, January 23, 2007; 68(4): 283 - 287.
[Abstract] [Full Text] [PDF]
B. P Liu, W. B.J Cafferty, S. O Budel, and S. M Strittmatter
Extracellular regulators of axonal growth in the adult central nervous system
Phil Trans R Soc B, September 29, 2006; 361(1473): 1593 - 1610.
[Abstract] [Full Text] [PDF]
I. C Maier and M. E Schwab
Sprouting, regeneration and circuit formation in the injured spinal cord: factors and activity
Phil Trans R Soc B, September 29, 2006; 361(1473): 1611 - 1634.
[Abstract] [Full Text] [PDF]
J. M. Massey, C. H. Hubscher, M. R. Wagoner, J. A. Decker, J. Amps, J. Silver, and S. M. Onifer
Chondroitinase ABC digestion of the perineuronal net promotes functional collateral sprouting in the cuneate nucleus after cervical spinal cord injury.
J. Neurosci., April 19, 2006; 26(16): 4406 - 4414.
[Abstract] [Full Text] [PDF]
C. M. Papadopoulos, S.-Y. Tsai, J. L. Cheatwood, M. R. Bollnow, B. E. Kolb, M. E. Schwab, and G. L. Kartje
Dendritic Plasticity in the Adult Rat Following Middle Cerebral Artery Occlusion and Nogo-A Neutralization
Cereb Cortex, April 1, 2006; 16(4): 529 - 536.
[Abstract] [Full Text] [PDF]
A. E. Lovett-Racke, P. D. Cravens, A. R. Gocke, M. K. Racke, and O. Stuve
Therapeutic Potential of Small Interfering RNA for Central Nervous System Diseases
Arch Neurol, December 1, 2005; 62(12): 1810 - 1813.
[Full Text] [PDF]
J. M. Schwab, P. P. Monnier, H. J. Schluesener, S. Conrad, R. Beschorner, L. Chen, R. Meyermann, and B. K. Mueller
Central Nervous System Injury-Induced Repulsive Guidance Molecule Expression in the Adult Human Brain
Arch Neurol, October 1, 2005; 62(10): 1561 - 1568.
[Abstract] [Full Text] [PDF]
L. Corvetti and F. Rossi
Degradation of Chondroitin Sulfate Proteoglycans Induces Sprouting of Intact Purkinje Axons in the Cerebellum of the Adult Rat
J. Neurosci., August 3, 2005; 25(31): 7150 - 7158.
[Abstract] [Full Text] [PDF]
L. L. Jones, D. Sajed, and M. H. Tuszynski
Axonal Regeneration through Regions of Chondroitin Sulfate Proteoglycan Deposition after Spinal Cord Injury: A Balance of Permissiveness and Inhibition
J. Neurosci., October 15, 2003; 23(28): 9276 - 9288.
[Abstract] [Full Text] [PDF]
L. Bouslama-Oueghlani, R. Wehrle, C. Sotelo, and I. Dusart
The Developmental Loss of the Ability of Purkinje Cells to Regenerate Their Axons Occurs in the Absence of Myelin: An In Vitro Model to Prevent Myelination
J. Neurosci., September 10, 2003; 23(23): 8318 - 8329.
[Abstract] [Full Text] [PDF]
T. Oertle, M. E. van der Haar, C. E. Bandtlow, A. Robeva, P. Burfeind, A. Buss, A. B. Huber, M. Simonen, L. Schnell, C. Brosamle, et al.
Nogo-A Inhibits Neurite Outgrowth and Cell Spreading with Three Discrete Regions
J. Neurosci., July 2, 2003; 23(13): 5393 - 5406.
[Abstract] [Full Text] [PDF]
A. J. Emerick, E. J. Neafsey, M. E. Schwab, and G. L. Kartje
Functional Reorganization of the Motor Cortex in Adult Rats after Cortical Lesion and Treatment with Monoclonal Antibody IN-1
J. Neurosci., June 15, 2003; 23(12): 4826 - 4830.
[Abstract] [Full Text] [PDF]
S. Gianola, T. Savio, M. E. Schwab, and F. Rossi
Cell-Autonomous Mechanisms and Myelin-Associated Factors Contribute to the Development of Purkinje Axon Intracortical Plexus in the Rat Cerebellum
J. Neurosci., June 1, 2003; 23(11): 4613 - 4624.
[Abstract] [Full Text] [PDF]
M. Fiedler, C. Horn, C. Bandtlow, M.E. Schwab, and A. Skerra
An engineered IN-1 Fab fragment with improved affinity for the Nogo-A axonal growth inhibitor permits immunochemical detection and shows enhanced neutralizing activity
Protein Eng. Des. Sel., November 1, 2002; 15(11): 931 - 941.
[Abstract] [Full Text] [PDF]
F. M. Bareyre, B. Haudenschild, and M. E. Schwab
Long-Lasting Sprouting and Gene Expression Changes Induced by the Monoclonal Antibody IN-1 in the Adult Spinal Cord
J. Neurosci., August 15, 2002; 22(16): 7097 - 7110.
[Abstract] [Full Text] [PDF]
A. B. Huber, O. Weinmann, C. Brosamle, T. Oertle, and M. E. Schwab
Patterns of Nogo mRNA and Protein Expression in the Developing and Adult Rat and After CNS Lesions
J. Neurosci., May 1, 2002; 22(9): 3553 - 3567.
[Abstract] [Full Text] [PDF]
T. Grandpre and S. M. Strittmatter
Nogo: A Molecular Determinant of Axonal Growth and Regeneration
Neuroscientist, October 1, 2001; 7(5): 377 - 386.
[Abstract] [PDF]
D. Ricard, V. Rogemond, E. Charrier, M. Aguera, D. Bagnard, M.-F. Belin, N. Thomasset, and J. Honnorat
Isolation and Expression Pattern of Human Unc-33-Like Phosphoprotein 6/Collapsin Response Mediator Protein 5 (Ulip6/CRMP5): Coexistence with Ulip2/CRMP2 in Sema3A- Sensitive Oligodendrocytes
J. Neurosci., September 15, 2001; 21(18): 7203 - 7214.
[Abstract] [Full Text] [PDF]
M. L. Condic
Adult Neuronal Regeneration Induced by Transgenic Integrin Expression
J. Neurosci., July 1, 2001; 21(13): 4782 - 4788.
[Abstract] [Full Text] [PDF]
O. Raineteau, K. Fouad, P. Noth, M. Thallmair, and M. E. Schwab
Functional switch between motor tracts in the presence of the mAb IN-1 in the adult rat
PNAS, May 24, 2001; (2001) 111165498.
[Abstract] [Full Text] [PDF]
D. Merkler, G. A. S. Metz, O. Raineteau, V. Dietz, M. E. Schwab, and K. Fouad
Locomotor Recovery in Spinal Cord-Injured Rats Treated with an Antibody Neutralizing the Myelin-Associated Neurite Growth Inhibitor Nogo-A
J. Neurosci., May 15, 2001; 21(10): 3665 - 3673.
[Abstract] [Full Text] [PDF]
M. S. Ramer, I. Duraisingam, J. V. Priestley, and S. B. McMahon
Two-Tiered Inhibition of Axon Regeneration at the Dorsal Root Entry Zone
J. Neurosci., April 15, 2001; 21(8): 2651 - 2660.
[Abstract] [Full Text] [PDF]
M. Vinson, P. J. L. M. Strijbos, A. Rowles, L. Facci, S. E. Moore, D. L. Simmons, and F. S. Walsh
Myelin-associated Glycoprotein Interacts with Ganglioside GT1b. A MECHANISM FOR NEURITE OUTGROWTH INHIBITION
J. Biol. Chem., June 1, 2001; 276(23): 20280 - 20285.
[Abstract] [Full Text] [PDF]
O. Raineteau, K. Fouad, P. Noth, M. Thallmair, and M. E. Schwab
Functional switch between motor tracts in the presence of the mAb IN-1 in the adult rat
PNAS, June 5, 2001; 98(12): 6929 - 6934.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|