 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
The Journal of Neuroscience, January 1, 2001, 21(1):27-34
Involvement of the Secretory Pathway for AMPA Receptors in NMDA-Induced Potentiation in Hippocampus
Greg Broutman and Michel Baudry Neuroscience Program, University of Southern California, Los Angeles, California 90089-2520
A chemical form of synaptic potentiation was produced with a brief bath application of NMDA to rat hippocampal slices. Two methods were used to assess changes in membrane-bound AMPA receptors. Traditional subcellular fractionation was used to isolate synaptic membranes; alternatively, membrane receptors were cross-linked with the membrane-impermeable reagent bis(sulfosuccinimidyl) suberate, and levels of nonmembrane receptors were determined. In both cases, Western blots were used to determine the content of receptor subunits in various subcellular fractions. NMDA-induced potentiation was associated with increased levels of glutamate receptor 1 (GluR1) and GluR2/3 subunits of AMPA receptors in synaptic membrane preparations, whereas no change was observed in whole homogenates. Both KN-62, an inhibitor of calcium/calmodulin kinase, and calpain inhibitor III, a calpain inhibitor, inhibited NMDA-induced potentiation and changes in GluR1 and GluR2/3 subunits of AMPA receptors. Brefeldin A (BFA) inhibits protein trafficking between the Golgi apparatus and cell membranes. Pretreatment of hippocampal slices with BFA significantly decreased NMDA-induced potentiation and completely prevented an NMDA-induced increase in GluR1 levels in membrane fractions. Thus, the levels of GluR1 and GluR2/3 subunits of AMPA receptors are rapidly upregulated in synaptic membranes under conditions associated with potentiation of synaptic responses, and this upregulation requires a functional secretory pathway.
Key words: AMPA receptors; brefeldin A; exocytosis; long-term potentiation; Golgi; hippocampal slice
Copyright © 2001 Society for Neuroscience 0270-6474/01/21127-08$05.00/0
This article has been cited by other articles:
S. Qiu, A. K. Jebelli, J. H. Ashe, and M. C. Curras-Collazo
Domoic Acid Induces a Long-Lasting Enhancement of CA1 Field Responses and Impairs Tetanus-Induced Long-term Potentiation in Rat Hippocampal Slices
Toxicol. Sci., September 1, 2009; 111(1): 140 - 150.
[Abstract] [Full Text] [PDF]
H. Jourdi, Y.-T. Hsu, M. Zhou, Q. Qin, X. Bi, and M. Baudry
Positive AMPA Receptor Modulation Rapidly Stimulates BDNF Release and Increases Dendritic mRNA Translation
J. Neurosci., July 8, 2009; 29(27): 8688 - 8697.
[Abstract] [Full Text] [PDF]
S. Qiu and E. J. Weeber
Reelin Signaling Facilitates Maturation of CA1 Glutamatergic Synapses
J Neurophysiol, March 1, 2007; 97(3): 2312 - 2321.
[Abstract] [Full Text] [PDF]
S. Qiu, L. F. Zhao, K. M. Korwek, and E. J. Weeber
Differential Reelin-Induced Enhancement of NMDA and AMPA Receptor Activity in the Adult Hippocampus
J. Neurosci., December 13, 2006; 26(50): 12943 - 12955.
[Abstract] [Full Text] [PDF]
A. C. Boudreau and M. E. Wolf
Behavioral Sensitization to Cocaine Is Associated with Increased AMPA Receptor Surface Expression in the Nucleus Accumbens
J. Neurosci., October 5, 2005; 25(40): 9144 - 9151.
[Abstract] [Full Text] [PDF]
A. A. Bagal, J. P. Y. Kao, C.-M. Tang, and S. M. Thompson
Long-term potentiation of exogenous glutamate responses at single dendritic spines
PNAS, October 4, 2005; 102(40): 14434 - 14439.
[Abstract] [Full Text] [PDF]
H. Jourdi, X. Lu, T. Yanagihara, J. C. Lauterborn, X. Bi, C. M. Gall, and M. Baudry
Prolonged Positive Modulation of {alpha}-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptors Induces Calpain-Mediated PSD-95/Dlg/ZO-1 Protein Degradation and AMPA Receptor Down-Regulation in Cultured Hippocampal Slices
J. Pharmacol. Exp. Ther., July 1, 2005; 314(1): 16 - 26.
[Abstract] [Full Text] [PDF]
S. M. Rodrigues, C. R. Farb, E. P. Bauer, J. E. LeDoux, and G. E. Schafe
Pavlovian Fear Conditioning Regulates Thr286 Autophosphorylation of Ca2+/Calmodulin-Dependent Protein Kinase II at Lateral Amygdala Synapses
J. Neurosci., March 31, 2004; 24(13): 3281 - 3288.
[Abstract] [Full Text] [PDF]
B. BARTHOLOME, C. M. SPIES, T. GABER, S. SCHUCHMANN, T. BERKI, D. KUNKEL, M. BIENERT, A. RADBRUCH, G.-R. BURMESTER, R. LAUSTER, et al.
Membrane glucocorticoid receptors (mGCR) are expressed in normal human peripheral blood mononuclear cells and up-regulated after in vitro stimulation and in patients with rheumatoid arthritis
FASEB J, January 1, 2004; 18(1): 70 - 80.
[Abstract] [Full Text] [PDF]
P. V. Perestenko and J. M. Henley
Characterization of the Intracellular Transport of GluR1 and GluR2 {alpha}-Amino-3-hydroxy-5-methyl-4-isoxazole Propionic Acid Receptor Subunits in Hippocampal Neurons
J. Biol. Chem., October 31, 2003; 278(44): 43525 - 43532.
[Abstract] [Full Text] [PDF]
J. A. Esteban
AMPA Receptor Trafficking: A Road Map for Synaptic Plasticity
Mol. Interv., October 1, 2003; 3(7): 375 - 385.
[Abstract] [Full Text] [PDF]
M. Hirasawa and Q. J. Pittman
From the Cover: Nifedipine facilitates neurotransmitter release independently of calcium channels
PNAS, May 13, 2003; 100(10): 6139 - 6144.
[Abstract] [Full Text] [PDF]
B. Lin, A. C. Arai, G. Lynch, and C. M. Gall
Integrins Regulate NMDA Receptor-Mediated Synaptic Currents
J Neurophysiol, May 1, 2003; 89(5): 2874 - 2878.
[Abstract] [Full Text] [PDF]
E. A. Kramar, J. A. Bernard, C. M. Gall, and G. Lynch
Integrins Modulate Fast Excitatory Transmission at Hippocampal Synapses
J. Biol. Chem., March 14, 2003; 278(12): 10722 - 10730.
[Abstract] [Full Text] [PDF]
J. Ko, S. Kim, J. G. Valtschanoff, H. Shin, J.-R. Lee, M. Sheng, R. T. Premont, R. J. Weinberg, and E. Kim
Interaction between Liprin-alpha and GIT1 Is Required for AMPA Receptor Targeting
J. Neurosci., March 1, 2003; 23(5): 1667 - 1677.
[Abstract] [Full Text] [PDF]
L. Fang, J. Wu, Q. Lin, and W. D. Willis
Calcium-Calmodulin-Dependent Protein Kinase II Contributes to Spinal Cord Central Sensitization
J. Neurosci., May 15, 2002; 22(10): 4196 - 4204.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|