WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience MBF Bioscience Autoneuron
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yoshikawa, S.
Right arrow Articles by Thomas, J. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoshikawa, S.
Right arrow Articles by Thomas, J. B.

 Previous Article  |  Next Article 

The Journal of Neuroscience, 2001, 21:RC119:1-4

RAPID COMMUNICATION
The Derailed Guidance Receptor Does Not Require Kinase Activity In Vivo

Shingo Yoshikawa, Joshua L. Bonkowsky, Michelle Kokel, Stanley Shyn, and John B. Thomas

Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, San Diego, California 92186

The Drosophila Derailed (DRL) receptor tyrosine kinase (RTK) controls key guidance events in the developing nervous system and mesoderm. Like other members of the "related to tyrosine kinases" (RYK) subfamily of RTKs, DRL has several highly unusual amino acid substitutions within the catalytic domain, raising the possibility that members of this subfamily are catalytically inactive. To test the role of DRL kinase activity in vivo, we mutated the invariant lysine required for catalytic activity of known kinases and examined the ability of this mutant to function in two assays: a dominant gain-of-function axon switch assay in the nervous system and phenotypic rescue of muscle attachment in drl mutants. We show that this predicted kinase-deficient DRL mutant is capable of functioning in both assays. Our results indicate that DRL does not require kinase activity in vivo and suggest that members of the RYK subfamily of RTKs transduce signals unconventionally.

Key words: receptor tyrosine kinase; kinase; Drosophila; axon guidance; Derailed; midline

Copyright © 0000 Society for Neuroscience  0270-6474/00/$05.00/0


This article has been cited by other articles:


Home page
 DevelopmentHome page
R. R. Wouda, M. R. K. S. Bansraj, A. W. M. de Jong, J. N. Noordermeer, and L. G. Fradkin
Src family kinases are required for WNT5 signaling through the Derailed/RYK receptor in the Drosophila embryonic central nervous system
Development, July 1, 2008; 135(13): 2277 - 2287.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
K. E. Harris and S. K. Beckendorf
Different Wnt signals act through the Frizzled and RYK receptors during Drosophila salivary gland migration
Development, June 1, 2007; 134(11): 2017 - 2025.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
L.-W. Tam, N. F. Wilson, and P. A. Lefebvre
A CDK-related kinase regulates the length and assembly of flagella in Chlamydomonas
J. Cell Biol., March 12, 2007; 176(6): 819 - 829.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Matsuoka, H. Obama, M. L. Kelly, T. Matsui, and M. Nakamoto
Biphasic Functions of the Kinase-defective Ephb6 Receptor in Cell Adhesion and Migration
J. Biol. Chem., August 12, 2005; 280(32): 29355 - 29363.
[Abstract] [Full Text] [PDF]


-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-