 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
The Journal of Neuroscience, May 15, 2001, 21(10):3483-3491
Immature Neurons From CNS Stem Cells Proliferate in Response to Platelet-Derived Growth Factor
Anna Erlandsson, Maria Enarsson, and Karin Forsberg-Nilsson Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, SE-751 85 Uppsala, Sweden
Identifying external signals involved in the regulation of neural stem cell proliferation and differentiation is fundamental to the understanding of CNS development. In this study we show that platelet-derived growth factor (PDGF) can act as a mitogen for neural precursor cells. Multipotent stem cells from developing CNS can be maintained in a proliferative state under serum-free conditions in the presence of fibroblast growth factor-2 (FGF2) and induced to differentiate into neurons, astrocytes, and oligodendrocytes on withdrawal of the mitogen. PDGF has been suggested to play a role during the differentiation into neurons. We have investigated the effect of PDGF on cultured stem cells from embryonic rat cortex. The PDGF
-receptor is constantly expressed during differentiation of neural stem cells but is phosphorylated only after PDGF-AA treatment. In contrast, the PDGF
-receptor is hardly detectable in uncommitted cells, but its expression increases during differentiation. We show that PDGF stimulation leads to c-fos induction, 5'-bromo-2'deoxyuridine incorporation, and an increase in the number of immature cells stained with antibodies to neuronal markers. Our findings suggest that PDGF acts as a mitogen in the early phase of stem cell differentiation to expand the pool of immature neurons.
Key words: PDGF; neural stem cells; differentiation; neurogenesis; rat; cortex; development
Copyright © 2001 Society for Neuroscience 0270-6474/01/21103483-09$05.00/0
This article has been cited by other articles:
A. Erlandsson, K. Brannvall, S. Gustafsdottir, B. Westermark, and K. Forsberg-Nilsson
Autocrine/Paracrine Platelet-Derived Growth Factor Regulates Proliferation of Neural Progenitor Cells
Cancer Res., August 15, 2006; 66(16): 8042 - 8048.
[Abstract] [Full Text] [PDF]
R.-S. Ge, Q. Dong, C. M. Sottas, V. Papadopoulos, B. R. Zirkin, and M. P. Hardy
In search of rat stem Leydig cells: Identification, isolation, and lineage-specific development
PNAS, February 21, 2006; 103(8): 2719 - 2724.
[Abstract] [Full Text] [PDF]
C De Bustos, A Smits, B Stromberg, V P Collins, M Nister, and G Afink
A PDGFRA promoter polymorphism, which disrupts the binding of ZNF148, is associated with primitive neuroectodermal tumours and ependymomas
J. Med. Genet., January 1, 2005; 42(1): 31 - 37.
[Abstract] [Full Text] [PDF]
Y. Hirabayashi, Y. Itoh, H. Tabata, K. Nakajima, T. Akiyama, N. Masuyama, and Y. Gotoh
The Wnt/{beta}-catenin pathway directs neuronal differentiation of cortical neural precursor cells
Development, June 15, 2004; 131(12): 2791 - 2801.
[Abstract] [Full Text] [PDF]
M. Enarsson, A. Erlandsson, H. Larsson, and K. Forsberg-Nilsson
Extracellular Signal-Regulated Protein Kinase Signaling Is Uncoupled From Initial Differentiation of Central Nervous System Stem Cells to Neurons
Mol. Cancer Res., December 1, 2002; 1(2): 147 - 154.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|