QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (26) Citing Articles via Google Scholar Google Scholar Articles by Lee, M. Articles by Waschek, J. Search for Related Content PubMed PubMed Citation Articles by Lee, M. Articles by Waschek, J.

 Previous Article  |  Next Article 

The Journal of Neuroscience, June 1, 2001, 21(11):3849-3859

Pituitary Adenylyl Cyclase-Activating Polypeptide Stimulates DNA Synthesis But Delays Maturation of Oligodendrocyte Progenitors

Matthew Lee¹, Vincent Lelièvre¹, Paul Zhao¹, Mike Torres², Williams Rodriguez¹, Ji-Yun Byun¹, Sameer Doshi¹, Yevgenyia Ioffe¹, Gauree Gupta¹, Araceli Espinosa de los Monteros¹, Jean de Vellis¹, and James Waschek¹

¹ Departments of Neurobiology and Psychiatry and Mental Retardation Research Center and ² Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, California 90024-1759

The neuropeptide pituitary adenylyl cyclase-activating peptide (PACAP) and one of its receptors (PAC₁) are expressed in embryonic neural tube, where they appear to regulate neurogenesis and patterning. We now show that PAC₁ gene expression is also present in neonatal rats in the ventricular and subventricular zones and in the optic chiasm, areas that are rich in oligodendrocyte (OL) progenitors (OLP). Because actions of PACAP on OLP have not been reported, we examined the effects of PACAP on the proliferation of purified OLP in culture and on myelinogenesis in cerebellar slices. Northern analyses on total RNA from purified glial cell subtypes revealed an abundant 7 kb hybridizing transcript in OLP, which was confirmed to correspond to the PAC₁ receptor by reverse transcription-PCR. The presence of this receptor was also corroborated by radioligand binding and cAMP assay. In cultured OL, receptor density decreased during maturation but was partially counterbalanced by the appearance of sites that bound both PACAP and the related peptide vasoactive intestinal peptide. PACAP increased DNA synthesis in OLP cultures almost twofold and increased the bromodeoxyuridine-labeling index in O4-positive OLP. PACAP treatment also resulted in decreased sulfate incorporation into sulfatide in cultures of differentiating OL. The PACAP effect on sulfatide synthesis was fully reproduced in a cerebellar explant model. These findings indicate that PACAP may act at two stages during OL development to (1) stimulate proliferation and (2) delay maturation and/or myelinogenesis.

Key words: PACAP receptors (PAC₁); PACAP; oligodendrocytes; proliferation; cAMP; myelination

---

Copyright © 2001 Society for Neuroscience  0270-6474/01/21113849-11$05.00/0

This article has been cited by other articles:

				Y.-V. Tan, C. Abad, R. Lopez, H. Dong, S. Liu, A. Lee, R. P. Gomariz, J. Leceta, and J. A. Waschek Pituitary adenylyl cyclase-activating polypeptide is an intrinsic regulator of Treg abundance and protects against experimental autoimmune encephalomyelitis PNAS, February 10, 2009; 106(6): 2012 - 2017. [Abstract] [Full Text] [PDF]

				D. K. Meyer, C. Fischer, U. Becker, I. Gottsching, S. Boutillier, C. Baermann, G. Schmidt, N. Klugbauer, and J. Leemhuis Pituitary Adenylyl Cyclase-activating Polypeptide 38 Reduces Astroglial Proliferation by Inhibiting the GTPase RhoA J. Biol. Chem., July 1, 2005; 280(26): 25258 - 25266. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help