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Previous Article | Next Article
The Journal of Neuroscience, June 15, 2001, 21(12):4290-4298
Repellent Signaling by Slit Requires the Leucine-Rich Repeats
Robin Battye, Adrienne Stevens, Robert L. Perry, and J. Roger Jacobs Department of Biology, McMaster University, Hamilton Ontario, L8S 4K1 Canada
Slit is a repellent axon guidance cue produced by the midline glia in Drosophila that is required to regulate the formation of contralateral projections and the lateral position of longitudinal tracts. Four sequence motifs comprise the structure of Slit: a leucine-rich repeat (LRR), epidermal growth factor-like (EGF) repeats, a laminin-like globular (G)-domain, and a cysteine domain. Here we demonstrate that the LRR is required for repellent signaling and in vitro binding to Robo. Repellent signaling by slit is reduced by point mutations that encode single amino acid changes in the LRR domain. By contrast to the EGF or G-domains, the LRR domain is required in transgenes to affect axon guidance. Finally, we show that the midline repellent receptor, Robo, binds Slit proteins with internal deletions that also retain repellent activity. However, Robo does not bind Slit protein missing the LRR. Taken together, our data demonstrate that Robo binding and repellent signaling by Slit require the LRR region.
Key words: axon guidance; Drosophila; midline glia; Slit; repulsion; Roundabout
Copyright © 2001 Society for Neuroscience 0270-6474/01/21124290-09$05.00/0
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