QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (28) Citing Articles via Google Scholar Google Scholar Articles by Heidelberger, R. Search for Related Content PubMed PubMed Citation Articles by Heidelberger, R.

 Previous Article  |  Next Article 

The Journal of Neuroscience, September 1, 2001, 21(17):6467-6474

ATP Is Required at an Early Step in Compensatory Endocytosis in Synaptic Terminals

Ruth Heidelberger

Department of Neurobiology and Anatomy, W. M. Keck Center for the Neurobiology of Learning and Memory, University of Texas Houston Health Science Center, Houston, Texas 77030

Whole-terminal capacitance measurements were used to examine membrane retrieval that follows Ca²⁺-triggered exocytosis in single synaptic terminals. Exocytosis was followed by endocytosis only when the internal solution contained a hydrolyzable analog of ATP. ATP--S, a poorly hydrolyzable ATP analog, did not support endocytosis but instead produced a rapid and profound inhibition of membrane retrieval. Under similar conditions, the GTP analogs GTP--S and GDP--S failed to block endocytosis, suggesting that ATP is the preferred substrate. Furthermore, the requirement for ATP was independent of the role of ATP in regulating intraterminal Ca²⁺, and the role of Ca²⁺ in endocytosis was different from that of ATP. The results suggest a direct, acute requirement for ATP hydrolysis in compensatory fast endocytosis in synaptic terminals. Given that the capacitance technique detects changes in membrane surface area, ATP must be required for the membrane fission step or at a step that is a prerequisite for membrane fission.

Key words: endocytosis; synaptic vesicle; ATP; synapse; bipolar cell; retina; calcium

---

Copyright © 2001 Society for Neuroscience  0270-6474/01/21176467-08$05.00/0

This article has been cited by other articles:

				G. Matthews and P. Sterling Evidence That Vesicles Undergo Compound Fusion on the Synaptic Ribbon J. Neurosci., May 21, 2008; 28(21): 5403 - 5411. [Abstract] [Full Text] [PDF]

				J. A. Hickman, J. M. Hardwick, L. K. Kaczmarek, and E. A. Jonas Bcl-xL Inhibitor ABT-737 Reveals a Dual Role for Bcl-xL in Synaptic Transmission J Neurophysiol, March 1, 2008; 99(3): 1515 - 1522. [Abstract] [Full Text] [PDF]

				H. Li, Y. Chen, A. F. Jones, R. H. Sanger, L. P. Collis, R. Flannery, E. C. McNay, T. Yu, R. Schwarzenbacher, B. Bossy, et al. Bcl-xL induces Drp1-dependent synapse formation in cultured hippocampal neurons PNAS, February 12, 2008; 105(6): 2169 - 2174. [Abstract] [Full Text] [PDF]

				B. Innocenti and R. Heidelberger Mechanisms Contributing to Tonic Release at the Cone Photoreceptor Ribbon Synapse J Neurophysiol, January 1, 2008; 99(1): 25 - 36. [Abstract] [Full Text] [PDF]

				E. Jonas BCL-xL Regulates Synaptic Plasticity Mol. Interv., August 1, 2006; 6(4): 208 - 222. [Abstract] [Full Text] [PDF]

				T. Yamashita, T. Hige, and T. Takahashi Vesicle Endocytosis Requires Dynamin-Dependent GTP Hydrolysis at a Fast CNS Synapse Science, January 7, 2005; 307(5706): 124 - 127. [Abstract] [Full Text] [PDF]

				R. Rikhy, M. Ramaswami, and K. S. Krishnan A Temperature-Sensitive Allele of Drosophila sesB Reveals Acute Functions for the Mitochondrial Adenine Nucleotide Translocase in Synaptic Transmission and Dynamin Regulation Genetics, November 1, 2003; 165(3): 1243 - 1253. [Abstract] [Full Text] [PDF]

				E. A. Jonas, D. Hoit, J. A. Hickman, T. A. Brandt, B. M. Polster, Y. Fannjiang, E. McCarthy, M. K. Montanez, J. M. Hardwick, and L. K. Kaczmarek Modulation of Synaptic Transmission by the BCL-2 Family Protein BCL-xL J. Neurosci., September 10, 2003; 23(23): 8423 - 8431. [Abstract] [Full Text] [PDF]

				C. Paillart, J. Li, G. Matthews, and P. Sterling Endocytosis and Vesicle Recycling at a Ribbon Synapse J. Neurosci., May 15, 2003; 23(10): 4092 - 4099. [Abstract] [Full Text] [PDF]

				R. Heidelberger, Z.-Y. Zhou, and G. Matthews Multiple Components of Membrane Retrieval in Synaptic Terminals Revealed by Changes in Hydrostatic Pressure J Neurophysiol, November 1, 2002; 88(5): 2509 - 2517. [Abstract] [Full Text] [PDF]

				R. Heidelberger, P. Sterling, and G. Matthews Roles of ATP in Depletion and Replenishment of the Releasable Pool of Synaptic Vesicles J Neurophysiol, July 1, 2002; 88(1): 98 - 106. [Abstract] [Full Text] [PDF]

				M. E. Graham, D. W. O'Callaghan, H. T. McMahon, and R. D. Burgoyne Dynamin-dependent and dynamin-independent processes contribute to the regulation of single vesicle release kinetics and quantal size PNAS, May 14, 2002; 99(10): 7124 - 7129. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help