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The Journal of Neuroscience, September 1, 2001, 21(17):6522-6531
ATP P2X Receptor-Mediated Enhancement of Glutamate Release and
Evoked EPSCs in Dorsal Horn Neurons of the Rat Spinal Cord
Terumasa
Nakatsuka and
Jianguo G.
Gu
McKnight Brain Institute of the University of Florida and Division
of Neuroscience, Department of Oral Surgery, College of Dentistry,
University of Florida, Gainesville, Florida 32610
Presynaptic ATP P2X receptors have been proposed to play a role in
modulating glutamate release from the first sensory synapse in the
spinal cord. Using spinal cord slice preparations and patch-clamp recordings from dorsal horn neurons in lamina V of the rat spinal cord,
we showed that the activation of P2X receptors by  , -methylene-ATP (m-ATP) resulted in a large increase in the frequency of
spontaneous EPSCs (sEPSCs) and miniature EPSCs (mEPSCs). The increases
in mEPSC frequency by m-ATP were not blocked by the
Ca2+ channel blocker, 30 µM
La3+, but were abolished in a bath solution when
Ca2+ was omitted. The increases in mEPSC frequency
by m-ATP were blocked completely by the P2 receptor
antagonist pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (PPADS)
at 10 µM. Furthermore, the EPSCs evoked by dorsal root
stimulation were potentiated by m-ATP as well as by the ecto-ATPase inhibitor ARL67156 and were depressed in the presence of P2
receptor antagonists PPADS (10 µM) and suramin (5 µM). The effects of these compounds on the evoked EPSCs
were associated with the changes in glutamate release probability of
primary afferent central terminals. Our results indicate that
m-ATP-sensitive P2X receptors play a significant role in
modulating excitatory sensory synaptic transmission in the spinal cord,
and the potential role of endogenous ATP is suggested.
Key words:
ATP; purinergic receptors; EPSCs; glutamate release; primary afferent fibers; patch-clamp technique; spinal cord slice
preparation
Copyright © 2001 Society for Neuroscience 0270-6474/01/21176522-10$05.00/0
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