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The Journal of Neuroscience, October 15, 2001, 21(20):7909-7918

Gating Properties of Na_v1.7 and Na_v1.8 Peripheral Nerve Sodium Channels

Kausalia Vijayaragavan^{1, 2}, Michael E. O'Leary³, and Mohamed Chahine^{1, 2}

¹ Laval University, Faculty of Medicine, Sainte-Foy, Québec, Canada G1K 7P4, ² Québec Heart Institute, Laval Hospital, Research Center, Sainte-Foy, Québec, Canada G1V 4G5, and ³ Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson Medical College, Philadelphia, Pennsylvania 19107

Several distinct components of voltage-gated sodium current have been recorded from native dorsal root ganglion (DRG) neurons that display differences in gating and pharmacology. This study compares the electrophysiological properties of two peripheral nerve sodium channels that are expressed selectively in DRG neurons (Na_v1.7 and Na_v1.8). Recombinant Na_v1.7 and Na_v1.8 sodium channels were coexpressed with the auxiliary ₁ subunit in Xenopus oocytes. In this system coexpression of the ₁ subunit with Na_v1.7 and Na_v1.8 channels results in more rapid inactivation, a shift in midpoints of steady-state activation and inactivation to more hyperpolarizing potentials, and an acceleration of recovery from inactivation. The coinjection of ₁ subunit also significantly increases the expression of Na_v1.8 by sixfold but has no effect on the expression of Na_v1.7. In addition, a great percentage of Na_v1.8+₁ channels is observed to enter rapidly into the slow inactivated states, in contrast to Nav1.7+₁ channels. Consequently, the rapid entry into slow inactivation is believed to cause a frequency-dependent reduction of Na_v1.8+₁ channel amplitudes, seen during repetitive pulsing between 1 and 2 Hz. However, at higher frequencies (>20 Hz) Na_v1.8+₁ channels reach a steady state to ~42% of total current. The presence of this steady-state sodium channel activity, coupled with the high activation threshold (V_0.5 = 3.3 mV) of Na_v1.8+₁, could enable the nociceptive fibers to fire spontaneously after nerve injury.

Key words: Na_v1.7; Na_v1.8; peripheral nerve sodium channels; expression; dorsal root ganglion; nociception; Xenopus oocytes

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Copyright © 2001 Society for Neuroscience  0270-6474/01/21207909-10$05.00/0

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