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The Journal of Neuroscience, November 15, 2001, 21(22):9068-9076

Expression of cGMP-Specific Phosphodiesterase 9A mRNA in the Rat Brain

Svetlana G. Andreeva¹, Pieter Dikkes¹, Paul M. Epstein², and Paul A. Rosenberg¹

¹ Department of Neurology, Children's Hospital, Boston, Massachusetts 02115, and ² Department of Pharmacology, University of Connecticut Health Center, Farmington, Connecticut 06030

cGMP has been implicated in the regulation of many essential functions in the brain, such as synaptic plasticity, phototransduction, olfaction, and behavioral state. Cyclic nucleotide phosphodiesterase (PDE) hydrolysis of cGMP is the major mechanism underlying the clearance of cGMP and is likely to be important in any process that depends on intracellular cGMP. PDE9A has the highest affinity for cGMP of any PDE, and here we studied the localization of this enzyme in the rat brain using in situ hybridization. PDE9A mRNA is widely distributed throughout the brain with varying regional expression. The pattern of PDE9A mRNA expression closely resembles that of soluble guanylyl cyclase (sGC) in the rat brain, suggesting a possible functional association or coupling of these two enzymes in the regulation of cGMP levels. Most of the brain areas expressing PDE9A mRNA also contain neuronal nitric oxide synthase (NOS), the enzymatic source of NO and the principal activator of sGC. PDE9A is the only cGMP-specific PDE with significant expression in the forebrain, and as such is likely to play an important role in NO-cGMP signaling.

Key words: nitric oxide; guanylyl cyclase; in situ hybridization; olfaction; memory; learning; sleep; basal forebrain; magnocellular; preoptic

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Copyright © 2001 Society for Neuroscience  0270-6474/01/21229068-09$05.00/0

This article has been cited by other articles:

				A. T. Bender and J. A. Beavo Cyclic Nucleotide Phosphodiesterases: Molecular Regulation to Clinical Use Pharmacol. Rev., September 1, 2006; 58(3): 488 - 520. [Abstract] [Full Text] [PDF]

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