QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (29) Citing Articles via Google Scholar Google Scholar Articles by Staunton, J. Articles by Nonet, M. L. Search for Related Content PubMed PubMed Citation Articles by Staunton, J. Articles by Nonet, M. L.

 Previous Article  |  Next Article 

The Journal of Neuroscience, December 1, 2001, 21(23):9255-9264

Rabphilin Potentiates Soluble N-Ethylmaleimide Sensitive Factor Attachment Protein Receptor Function Independently of rab3

Jane Staunton^{1, 2}, Barry Ganetzky², and Michael L. Nonet¹

¹ Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110, and ² Department of Genetics, University of Wisconsin, Madison, Wisconsin 53705

Rabphilin, a putative rab effector, interacts specifically with the GTP-bound form of the synaptic vesicle-associated protein rab3a. In this study, we define in vivo functions for rabphilin through the characterization of mutants that disrupt the Caenorhabditis elegans rabphilin homolog. The mutants do not display the general synaptic defects associated with rab3 lesions, as assayed at the pharmacological, physiological, and ultrastructural level. However, rabphilin mutants exhibit severe lethargy in the absence of mechanical stimulation. Furthermore, rabphilin mutations display strong synergistic interactions with hypomorphic lesions in the syntaxin, synaptosomal-associated protein of 25 kDa, and synaptobrevin soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE) genes; double mutants were nonresponsive to mechanical stimulation. These synergistic interactions were independent of rab3 function and were not observed in rab3-SNARE double mutants. Our data reveal rab3-independent functions for rabphilin in the potentiation of SNARE function.

Key words: synaptic vesicle; GTPase effector; vesicle fusion; neurotransmitter release; SNARE; synaptobrevin; SNAP-25; syntaxin

---

Copyright © 2001 Society for Neuroscience  0270-6474/01/21239255-10$05.00/0

This article has been cited by other articles:

				P. Montaville, C. Schlicker, A. Leonov, M. Zweckstetter, G. M. Sheldrick, and S. Becker The C2A-C2B Linker Defines the High Affinity Ca2+ Binding Mode of Rabphilin-3A J. Biol. Chem., February 16, 2007; 282(7): 5015 - 5025. [Abstract] [Full Text] [PDF]

				T. R. Mahoney, Q. Liu, T. Itoh, S. Luo, G. Hadwiger, R. Vincent, Z.-W. Wang, M. Fukuda, and M. L. Nonet Regulation of Synaptic Transmission by RAB-3 and RAB-27 in Caenorhabditis elegans Mol. Biol. Cell, June 1, 2006; 17(6): 2617 - 2625. [Abstract] [Full Text] [PDF]

				T. Tsuboi and M. Fukuda The C2B Domain of Rabphilin Directly Interacts with SNAP-25 and Regulates the Docking Step of Dense Core Vesicle Exocytosis in PC12 Cells J. Biol. Chem., November 25, 2005; 280(47): 39253 - 39259. [Abstract] [Full Text] [PDF]

				G. Baldini, A. M. Martelli, G. Tabellini, C. Horn, K. Machaca, P. Narducci, and G. Baldini Rabphilin Localizes with the Cell Actin Cytoskeleton and Stimulates Association of Granules with F-actin Cross-linked by {alpha}-Actinin J. Biol. Chem., October 14, 2005; 280(41): 34974 - 34984. [Abstract] [Full Text] [PDF]

				R. Steven, L. Zhang, J. Culotti, and T. Pawson The UNC-73/Trio RhoGEF-2 domain is required in separate isoforms for the regulation of pharynx pumping and normal neurotransmission in C. elegans Genes & Dev., September 1, 2005; 19(17): 2016 - 2029. [Abstract] [Full Text] [PDF]

				M. Fukuda Versatile Role of Rab27 in Membrane Trafficking: Focus on the Rab27 Effector Families J. Biochem., January 1, 2005; 137(1): 9 - 16. [Abstract] [Full Text] [PDF]

				S. P. Koushika, A. M. Schaefer, R. Vincent, J. H. Willis, B. Bowerman, and M. L. Nonet Mutations in Caenorhabditis elegans Cytoplasmic Dynein Components Reveal Specificity of Neuronal Retrograde Cargo J. Neurosci., April 21, 2004; 24(16): 3907 - 3916. [Abstract] [Full Text] [PDF]

				M. Fukuda, E. Kanno, and A. Yamamoto Rabphilin and Noc2 Are Recruited to Dense-core Vesicles through Specific Interaction with Rab27A in PC12 Cells J. Biol. Chem., March 26, 2004; 279(13): 13065 - 13075. [Abstract] [Full Text] [PDF]

				M. P. Rastaldi, S. Armelloni, S. Berra, M. Li, M. Pesaresi, H. Poczewski, B. Langer, D. Kerjaschki, A. Henger, S. M. Blattner, et al. Glomerular Podocytes Possess the Synaptic Vesicle Molecule Rab3A and Its Specific Effector Rabphilin-3a Am. J. Pathol., September 1, 2003; 163(3): 889 - 899. [Abstract] [Full Text] [PDF]

				M. Fukuda Distinct Rab Binding Specificity of Rim1, Rim2, Rabphilin, and Noc2. IDENTIFICATION OF A CRITICAL DETERMINANT OF Rab3A/Rab27A RECOGNITION BY Rim2 J. Biol. Chem., April 18, 2003; 278(17): 15373 - 15380. [Abstract] [Full Text] [PDF]

				R. D. Burgoyne and A. Morgan Secretory Granule Exocytosis Physiol Rev, April 1, 2003; 83(2): 581 - 632. [Abstract] [Full Text] [PDF]

				S. Torii, S. Zhao, Z. Yi, T. Takeuchi, and T. Izumi Granuphilin Modulates the Exocytosis of Secretory Granules through Interaction with Syntaxin 1a Mol. Cell. Biol., August 1, 2002; 22(15): 5518 - 5526. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help