QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (16) Citing Articles via Google Scholar Google Scholar Articles by Becherer, U. Articles by Betz, W. J. Search for Related Content PubMed PubMed Citation Articles by Becherer, U. Articles by Betz, W. J.

 Previous Article  |  Next Article 

The Journal of Neuroscience, February 1, 2001, 21(3):782-787

Effects of Staurosporine on Exocytosis and Endocytosis at Frog Motor Nerve Terminals

Ute Becherer, Cristina Guatimosim, and William J. Betz

Department of Physiology and Biophysics, University of Colorado Medical School, Denver, Colorado 80262

Observations of the dynamic staining and destaining of FM1-43 in frog motor nerve terminals (Henkel and Betz, 1995) suggested that staurosporine might shorten the interval between exocytosis and endocytosis, inducing a "kiss and run" mode of exocytosis and endocytosis. We tested this hypothesis by using FM1-43 imaging (to measure the time course of FM1-43 endocytosis), intracellular recording of evoked synaptic potentials (to measure acetylcholine release), and electron microscopy (to examine synaptic vesicle distribution). Staurosporine reduced FM1-43 uptake during but not after a tetanus, increased the speed of end plate potential (EPP) amplitude rundown, and greatly slowed the recovery from synaptic depression. Ultrastructural observations showed pronounced vesicle depletion near active zones after tetanic stimulation in staurosporine-treated preparations. These results suggest that staurosporine acted primarily to impair mobilization of synaptic vesicles during tetanic stimulation.

Key words: staurosporine; exocytosis; endocytosis; synaptic vesicles; frog neuromuscular junction; FM1-43

---

Copyright © 2001 Society for Neuroscience  0270-6474/01/213782-06$05.00/0

This article has been cited by other articles:

				X. Wang, R. Thiagarajan, Q. Wang, T. Tewolde, M. M. Rich, and K. L. Engisch Regulation of quantal shape by Rab3A: evidence for a fusion pore-dependent mechanism J. Physiol., August 15, 2008; 586(16): 3949 - 3962. [Abstract] [Full Text] [PDF]

				E. J. Schwartz, T. Blackmer, T. Gerachshenko, and S. Alford Presynaptic G-Protein-Coupled Receptors Regulate Synaptic Cleft Glutamate via Transient Vesicle Fusion J. Neurosci., May 30, 2007; 27(22): 5857 - 5868. [Abstract] [Full Text] [PDF]

				R. Jordan, E. A. Lemke, and J. Klingauf Visualization of Synaptic Vesicle Movement in Intact Synaptic Boutons Using Fluorescence Fluctuation Spectroscopy Biophys. J., September 1, 2005; 89(3): 2091 - 2102. [Abstract] [Full Text] [PDF]

				L. Polo-Parada, C. M. Bose, F. Plattner, and L. T. Landmesser Distinct Roles of Different Neural Cell Adhesion Molecule (NCAM) Isoforms in Synaptic Maturation Revealed by Analysis of NCAM 180 kDa Isoform-Deficient Mice J. Neurosci., February 25, 2004; 24(8): 1852 - 1864. [Abstract] [Full Text] [PDF]

				T. Sakaba and E. Neher Involvement of Actin Polymerization in Vesicle Recruitment at the Calyx of Held Synapse J. Neurosci., February 1, 2003; 23(3): 837 - 846. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help