QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (62) Citing Articles via Google Scholar Google Scholar Articles by Teubner, B. Articles by Willecke, K. Search for Related Content PubMed PubMed Citation Articles by Teubner, B. Articles by Willecke, K.

 Previous Article  |  Next Article 

The Journal of Neuroscience, February 15, 2001, 21(4):1117-1126

Functional Expression of the New Gap Junction Gene Connexin47 Transcribed in Mouse Brain and Spinal Cord Neurons

Barbara Teubner¹, Benjamin Odermatt¹, Martin Güldenagel¹, Goran Söhl¹, Joachim Degen¹, Feliksas F. Bukauskas², Jack Kronengold², Vytas K. Verselis², Yong Tae Jung³, Christine A. Kozak³, Karl Schilling⁴, and Klaus Willecke¹

¹ Institut für Genetik, Universität Bonn, D-53117 Bonn, Germany, ² Department of Neuroscience, Albert Einstein College of Medicine, New York, New York 10461, ³ National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, and ⁴ Anatomisches Institut, Universität Bonn, D-53115 Bonn, Germany

A new mouse gap junction gene that codes for a protein of 46,551 Da has been identified and designated connexin47 (Cx47). It mapped as a single-copy gene to mouse chromosome 11. In human HeLa cells and Xenopus oocytes, expression of mouse Cx47 or a fusion protein of Cx47 and enhanced green fluorescent protein induced intercellular channels that displayed strong sensitivity to transjunctional voltage. Tracer injections in Cx47-transfected HeLa cells revealed intercellular diffusion of neurobiotin, Lucifer yellow, and 4',6-diamidino-2-phenylindole. Recordings of single channels yielded a unitary conductance of 55 pS main state and 8 pS substate. Cx47 mRNA expression was high in spinal cord and brain but was not found in retina, liver, heart, and lung. A low level of Cx47 expression was detected in ovaries. In situ hybridizations demonstrated high expression in  motor neurons of the spinal cord, pyramidal cells of the cortex and hippocampus, granular and molecular layers of the dentate gyrus, and Purkinje cells of the cerebellum as well as several nuclei of the brainstem. This expression pattern is distinct from, although partially overlapping with, that of the neuronally expressed connexin36 gene. Thus, electrical synapses in adult mammalian brain are likely to consist of different connexin proteins depending on the neuronal subtype.

Key words: Cx47; gap junctions; neuronal connexin; electrical synapses; voltage gating; permeability

---

Copyright © 2001 Society for Neuroscience  0270-6474/01/2141117-10$05.00/0

This article has been cited by other articles:

				J. L. Orthmann-Murphy, M. Freidin, E. Fischer, S. S. Scherer, and C. K. Abrams Two Distinct Heterotypic Channels Mediate Gap Junction Coupling between Astrocyte and Oligodendrocyte Connexins J. Neurosci., December 19, 2007; 27(51): 13949 - 13957. [Abstract] [Full Text] [PDF]

				M. Bugiani, S. Al Shahwan, E. Lamantea, A. Bizzi, E. Bakhsh, I. Moroni, M. R. Balestrini, G. Uziel, and M. Zeviani GJA12 mutations in children with recessive hypomyelinating leukoencephalopathy Neurology, July 25, 2006; 67(2): 273 - 279. [Abstract] [Full Text] [PDF]

				P. Bedner, H. Niessen, B. Odermatt, M. Kretz, K. Willecke, and H. Harz Selective Permeability of Different Connexin Channels to the Second Messenger Cyclic AMP J. Biol. Chem., March 10, 2006; 281(10): 6673 - 6681. [Abstract] [Full Text] [PDF]

				S. Hidaka, Y. Akahori, and Y. Kurosawa Dendrodendritic Electrical Synapses between Mammalian Retinal Ganglion Cells J. Neurosci., November 17, 2004; 24(46): 10553 - 10567. [Abstract] [Full Text] [PDF]

				J. von Maltzahn, C. Euwens, K. Willecke, and G. Sohl The novel mouse connexin39 gene is expressed in developing striated muscle fibers J. Cell Sci., October 15, 2004; 117(22): 5381 - 5392. [Abstract] [Full Text] [PDF]

				G. Cheron, D. Gall, L. Servais, B. Dan, R. Maex, and S. N. Schiffmann Inactivation of Calcium-Binding Protein Genes Induces 160 Hz Oscillations in the Cerebellar Cortex of Alert Mice J. Neurosci., January 14, 2004; 24(2): 434 - 441. [Abstract] [Full Text] [PDF]

				D. M. Menichella, D. A. Goodenough, E. Sirkowski, S. S. Scherer, and D. L. Paul Connexins Are Critical for Normal Myelination in the CNS J. Neurosci., July 2, 2003; 23(13): 5963 - 5973. [Abstract] [Full Text] [PDF]

				B. Odermatt, K. Wellershaus, A. Wallraff, G. Seifert, J. Degen, C. Euwens, B. Fuss, H. Bussow, K. Schilling, C. Steinhauser, et al. Connexin 47 (Cx47)-Deficient Mice with Enhanced Green Fluorescent Protein Reporter Gene Reveal Predominant Oligodendrocytic Expression of Cx47 and Display Vacuolized Myelin in the CNS J. Neurosci., June 1, 2003; 23(11): 4549 - 4559. [Abstract] [Full Text] [PDF]

				G. Radnikow, D. Feldmeyer, and J. Lubke Axonal Projection, Input and Output Synapses, and Synaptic Physiology of Cajal-Retzius Cells in the Developing Rat Neocortex J. Neurosci., August 15, 2002; 22(16): 6908 - 6919. [Abstract] [Full Text] [PDF]

				B. M. Altevogt, K. A. Kleopa, F. R. Postma, S. S. Scherer, and D. L. Paul Connexin29 Is Uniquely Distributed within Myelinating Glial Cells of the Central and Peripheral Nervous Systems J. Neurosci., August 1, 2002; 22(15): 6458 - 6470. [Abstract] [Full Text] [PDF]

				N. Maier, M. Guldenagel, G. Sohl, H. Siegmund, K. Willecke, and A. Draguhn Reduction of high-frequency network oscillations (ripples) and pathological network discharges in hippocampal slices from connexin 36-deficient mice J. Physiol., June 1, 2002; 541(2): 521 - 528. [Abstract] [Full Text] [PDF]

				M. V. Frantseva, L. Kokarovtseva, C. G. Naus, P. L. Carlen, D. MacFabe, and J. L. Perez Velazquez Specific Gap Junctions Enhance the Neuronal Vulnerability to Brain Traumatic Injury J. Neurosci., February 1, 2002; 22(3): 644 - 653. [Abstract] [Full Text] [PDF]

				M. Guldenagel, J. Ammermuller, A. Feigenspan, B. Teubner, J. Degen, G. Sohl, K. Willecke, and R. Weiler Visual Transmission Deficits in Mice with Targeted Disruption of the Gap Junction Gene Connexin36 J. Neurosci., August 15, 2001; 21(16): 6036 - 6044. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help